Detailed information NTG0893882

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AAG092_RS11000
• Genome accession: NZ_CP154874
• Coordinates: 2263206..2264324 (+)
• Length: 372 a.a.
• NCBI ID: WP_373386692.1
• Uniprot ID: -
• Organism: Pseudomonas alcaligenes strain Med1
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2258206..2269324

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAG092_RS10995 (AAG092_10935)	recC	2259851..2263108 (-)	3258	WP_373386691.1	exodeoxyribonuclease V subunit gamma	-
AAG092_RS11000 (AAG092_10940)	pilU	2263206..2264324 (+)	1119	WP_373386692.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AAG092_RS11005 (AAG092_10945)	-	2264408..2264701 (+)	294	WP_373386694.1	hypothetical protein	-
AAG092_RS11010 (AAG092_10950)	-	2264868..2267123 (+)	2256	WP_373386695.1	putative bifunctional diguanylate cyclase/phosphodiesterase	-
AAG092_RS11015	-	2267120..2267449 (+)	330	Protein_2167	PAS domain-containing protein	-
AAG092_RS11020 (AAG092_10955)	-	2267579..2268709 (+)	1131	WP_373389587.1	methyl-accepting chemotaxis protein	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41491.64 Da
• Isoelectric Point: 6.3444

>NTDB_id=893882 AAG092_RS11000 WP_373386692.1 2263206..2264324(+) (pilU) [Pseudomonas alcaligenes strain Med1]
MPEPSLDVFDYLQRMRELQASDLFLSVGAPAHAKVEGLSQALDARVLGPGEVKQMAYQLLSQKQIAEFERDLELDLAVSM
NNQGRFRANLYYQRGEVAMVVRLIKSEIPDFEHLGLPKMLEKLAMQDRGLILVVGAAGSGKSTTLAAMIDFRNRHKGGHI
LCIEDPIEFLHSHQLSIVDQREVGLDTHSFEEALRHVLREAPDVIMLGEIRDAATMQHALHYAETGHLCLATLHATSSSH
AIERIVRFFPDGARKQVLADLAHNLLAVVGQRLVPGLAQKRVAAVELMLGTPYIRDLIQRDELEQLREATARAAEQGLQT
FDQHLFALVEGKRIGLAEALKFADSRTDLSLRFKLERGFGADDSERKVLRDG

Nucleotide

• Length: 1119 bp

>NTDB_id=893882 AAG092_RS11000 WP_373386692.1 2263206..2264324(+) (pilU) [Pseudomonas alcaligenes strain Med1]
ATGCCCGAGCCCAGCCTGGATGTCTTCGACTACCTGCAGCGCATGCGTGAGCTGCAGGCCTCCGACCTGTTCCTCAGCGT
CGGCGCGCCGGCCCACGCCAAGGTCGAAGGGCTCAGCCAGGCCCTCGACGCGCGCGTGCTGGGTCCCGGCGAGGTCAAGC
AGATGGCCTACCAGCTGCTCAGCCAGAAGCAGATCGCCGAGTTCGAGCGCGACCTGGAGCTGGACCTGGCGGTGAGCATG
AACAATCAGGGGCGCTTTCGCGCCAACCTGTACTACCAGCGCGGCGAAGTGGCCATGGTGGTACGCCTGATCAAGAGCGA
GATTCCCGACTTCGAGCACCTCGGCCTGCCGAAGATGCTGGAGAAACTGGCCATGCAGGACCGCGGCCTGATCCTGGTGG
TCGGCGCCGCCGGCTCCGGCAAGTCGACCACGCTGGCGGCGATGATCGACTTCCGCAACCGCCACAAGGGCGGCCATATC
CTGTGCATCGAGGACCCCATCGAGTTCCTCCACAGCCACCAGCTGTCCATCGTCGACCAGCGCGAGGTCGGCCTCGACAC
CCATAGTTTCGAGGAGGCCCTGCGCCACGTGCTGCGCGAGGCGCCGGACGTGATCATGCTCGGCGAGATCCGCGACGCCG
CGACCATGCAGCACGCCCTGCACTACGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACGCCACCAGCAGCAGCCAC
GCTATCGAGCGCATCGTGCGCTTCTTCCCCGACGGCGCGCGCAAGCAGGTGCTGGCCGACCTCGCGCACAACCTCCTGGC
GGTGGTCGGCCAGCGCCTGGTGCCGGGCCTGGCGCAGAAGCGCGTGGCGGCGGTGGAGCTGATGCTCGGCACGCCCTATA
TCCGCGACCTGATCCAGCGCGACGAACTGGAGCAGCTGCGCGAGGCCACCGCGCGCGCGGCCGAGCAGGGCCTGCAGACC
TTCGACCAGCACCTGTTCGCCCTGGTCGAAGGCAAACGCATCGGCCTGGCCGAGGCGCTCAAGTTCGCCGACTCGCGCAC
CGACCTGAGCCTGCGCTTCAAGCTGGAACGCGGCTTCGGCGCCGACGACAGCGAGCGCAAGGTGCTGCGCGACGGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (62-274)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Vibrio cholerae strain A1552	46.61	95.161	0.444
pilU	Pseudomonas stutzeri DSM 10701	46.957	92.742	0.435
pilU	Acinetobacter baylyi ADP1	44.11	98.118	0.433
pilT	Acinetobacter baylyi ADP1	40.588	91.398	0.371
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.284	87.097	0.368
pilT	Legionella pneumophila strain Lp02	41.212	88.71	0.366
pilT	Legionella pneumophila strain ERS1305867	41.212	88.71	0.366
pilT	Pseudomonas aeruginosa PAK	41.667	87.097	0.363
pilT	Vibrio cholerae strain A1552	41.358	87.097	0.36
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.358	87.097	0.36
pilT	Pseudomonas stutzeri DSM 10701	41.358	87.097	0.36