Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   RX143_RS08520 Genome accession   NZ_CP136360
Coordinates   1733451..1733726 (-) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain ES-3222     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1693046..1751831 1733451..1733726 within 0


Gene organization within MGE regions


Location: 1693046..1751831
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RX143_RS08225 (RX143_08225) - 1693046..1694053 (-) 1008 WP_010819472.1 class I SAM-dependent methyltransferase -
  RX143_RS08230 (RX143_08230) comGG 1694182..1694535 (-) 354 WP_002362054.1 competence type IV pilus minor pilin ComGG -
  RX143_RS08235 (RX143_08235) comGF 1694535..1694969 (-) 435 WP_002357060.1 competence type IV pilus minor pilin ComGF -
  RX143_RS08240 (RX143_08240) - 1694959..1695330 (-) 372 WP_171025395.1 type II secretion system protein -
  RX143_RS08245 (RX143_08245) - 1695663..1695788 (+) 126 WP_002364184.1 hypothetical protein -
  RX143_RS08250 (RX143_08250) hemH 1696085..1697026 (-) 942 WP_002364185.1 ferrochelatase -
  RX143_RS08260 (RX143_08260) - 1697766..1698014 (-) 249 WP_002383136.1 hypothetical protein -
  RX143_RS08265 (RX143_08265) - 1698086..1698286 (-) 201 WP_002357053.1 cold-shock protein -
  RX143_RS08270 (RX143_08270) - 1699123..1700364 (-) 1242 WP_002370962.1 LysM peptidoglycan-binding domain-containing protein -
  RX143_RS08275 (RX143_08275) - 1700365..1700598 (-) 234 WP_002384371.1 phage holin -
  RX143_RS08280 (RX143_08280) - 1700591..1700812 (-) 222 WP_316497558.1 hypothetical protein -
  RX143_RS08285 (RX143_08285) - 1700847..1701002 (-) 156 WP_002364192.1 XkdX family protein -
  RX143_RS08290 (RX143_08290) - 1701004..1701324 (-) 321 WP_002389262.1 hypothetical protein -
  RX143_RS08295 (RX143_08295) - 1701338..1701829 (-) 492 WP_010819473.1 hypothetical protein -
  RX143_RS08300 (RX143_08300) - 1701829..1702116 (-) 288 WP_002357046.1 collagen-like protein -
  RX143_RS08305 (RX143_08305) - 1702113..1702709 (-) 597 WP_002357045.1 hypothetical protein -
  RX143_RS08310 (RX143_08310) - 1702702..1703583 (-) 882 WP_010819474.1 phage baseplate upper protein -
  RX143_RS08315 (RX143_08315) - 1703602..1706409 (-) 2808 WP_002384367.1 phage tail spike protein -
  RX143_RS08320 (RX143_08320) - 1706391..1707125 (-) 735 WP_002403868.1 tail protein -
  RX143_RS08325 (RX143_08325) - 1707115..1710012 (-) 2898 WP_025189864.1 tape measure protein -
  RX143_RS08330 (RX143_08330) gpG 1710260..1710610 (-) 351 WP_010819476.1 phage tail assembly chaperone G -
  RX143_RS08335 (RX143_08335) - 1710663..1711511 (-) 849 WP_010819477.1 major tail protein -
  RX143_RS08340 (RX143_08340) - 1711512..1711886 (-) 375 WP_010716865.1 DUF6838 family protein -
  RX143_RS08345 (RX143_08345) - 1711889..1712287 (-) 399 WP_316497559.1 HK97 gp10 family phage protein -
  RX143_RS08350 (RX143_08350) - 1712280..1712648 (-) 369 WP_010707371.1 hypothetical protein -
  RX143_RS08355 (RX143_08355) - 1712645..1712989 (-) 345 WP_010819479.1 hypothetical protein -
  RX143_RS08360 (RX143_08360) - 1713003..1713185 (-) 183 WP_002357032.1 hypothetical protein -
  RX143_RS08365 (RX143_08365) - 1713214..1714101 (-) 888 WP_010819480.1 DUF5309 domain-containing protein -
  RX143_RS08370 (RX143_08370) - 1714115..1714738 (-) 624 WP_010707369.1 DUF4355 domain-containing protein -
  RX143_RS08375 (RX143_08375) - 1714879..1715142 (-) 264 WP_025189865.1 DUF6275 family protein -
  RX143_RS08380 (RX143_08380) - 1715210..1715530 (-) 321 WP_002414520.1 hypothetical protein -
  RX143_RS08385 (RX143_08385) - 1715587..1715817 (-) 231 WP_010819482.1 hypothetical protein -
  RX143_RS08390 (RX143_08390) - 1715818..1717719 (-) 1902 WP_010819483.1 phage minor head protein -
  RX143_RS08395 (RX143_08395) - 1717700..1719166 (-) 1467 WP_010819484.1 phage portal protein -
  RX143_RS08400 (RX143_08400) terL 1719179..1720567 (-) 1389 WP_201763236.1 phage terminase large subunit -
  RX143_RS08405 (RX143_08405) - 1720560..1721021 (-) 462 WP_010819486.1 helix-turn-helix domain-containing protein -
  RX143_RS08410 (RX143_08410) - 1721052..1721282 (-) 231 WP_010819487.1 hypothetical protein -
  RX143_RS08420 (RX143_08420) - 1722272..1722688 (-) 417 WP_002403876.1 ArpU family phage packaging/lysis transcriptional regulator -
  RX143_RS08425 (RX143_08425) - 1723595..1724029 (-) 435 WP_002402491.1 RusA family crossover junction endodeoxyribonuclease -
  RX143_RS08430 (RX143_08430) - 1724038..1724337 (-) 300 WP_002372047.1 MazG-like family protein -
  RX143_RS08435 (RX143_08435) - 1724338..1724640 (-) 303 WP_002368214.1 hypothetical protein -
  RX143_RS08440 (RX143_08440) - 1724637..1725509 (-) 873 WP_010715319.1 helix-turn-helix domain-containing protein -
  RX143_RS08445 (RX143_08445) - 1725509..1725709 (-) 201 WP_010715320.1 hypothetical protein -
  RX143_RS08450 (RX143_08450) - 1725714..1726355 (-) 642 WP_002402487.1 putative HNHc nuclease -
  RX143_RS08455 (RX143_08455) - 1726360..1727094 (-) 735 WP_002402486.1 ERF family protein -
  RX143_RS08460 (RX143_08460) - 1727087..1727404 (-) 318 WP_002402485.1 hypothetical protein -
  RX143_RS08465 (RX143_08465) - 1727600..1727938 (-) 339 WP_010819488.1 hypothetical protein -
  RX143_RS08470 (RX143_08470) - 1727975..1728184 (-) 210 WP_002378465.1 hypothetical protein -
  RX143_RS08475 (RX143_08475) - 1728239..1728427 (+) 189 WP_002357001.1 YegP family protein -
  RX143_RS08480 (RX143_08480) - 1728453..1729175 (-) 723 WP_002410531.1 Rha family transcriptional regulator -
  RX143_RS08485 (RX143_08485) - 1729198..1729509 (-) 312 WP_002403885.1 hypothetical protein -
  RX143_RS08490 (RX143_08490) - 1729521..1729712 (-) 192 WP_002383936.1 hypothetical protein -
  RX143_RS08495 (RX143_08495) - 1730008..1730349 (+) 342 WP_002387267.1 helix-turn-helix transcriptional regulator -
  RX143_RS08500 (RX143_08500) - 1730354..1731004 (+) 651 WP_025189866.1 ImmA/IrrE family metallo-endopeptidase -
  RX143_RS08505 (RX143_08505) - 1731100..1731729 (+) 630 WP_002387265.1 SHOCT domain-containing protein -
  RX143_RS08510 (RX143_08510) - 1731835..1732983 (+) 1149 WP_010819490.1 site-specific integrase -
  RX143_RS08515 (RX143_08515) comGD 1733020..1733454 (-) 435 Protein_1646 competence type IV pilus minor pilin ComGD -
  RX143_RS08520 (RX143_08520) comGC/cglC 1733451..1733726 (-) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene
  RX143_RS08525 (RX143_08525) comGB 1733726..1734772 (-) 1047 WP_010819492.1 competence type IV pilus assembly protein ComGB -
  RX143_RS08530 (RX143_08530) comGA 1734729..1735697 (-) 969 WP_010819493.1 competence type IV pilus ATPase ComGA -
  RX143_RS08535 (RX143_08535) - 1735937..1737265 (-) 1329 WP_002362058.1 APC family permease -
  RX143_RS08540 (RX143_08540) rlmN 1737555..1738628 (-) 1074 WP_002381713.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  RX143_RS08545 (RX143_08545) - 1738753..1740582 (-) 1830 WP_010819494.1 ABC transporter permease -
  RX143_RS08550 (RX143_08550) - 1740572..1741321 (-) 750 WP_002381711.1 ABC transporter ATP-binding protein -
  RX143_RS08555 (RX143_08555) - 1741439..1742140 (-) 702 WP_002356983.1 GntR family transcriptional regulator -
  RX143_RS08560 (RX143_08560) - 1742271..1744694 (-) 2424 WP_010819495.1 DNA translocase FtsK -
  RX143_RS08565 (RX143_08565) - 1745008..1746219 (-) 1212 WP_010819496.1 NAD(P)/FAD-dependent oxidoreductase -
  RX143_RS08570 (RX143_08570) - 1746248..1747153 (-) 906 WP_002360016.1 prenyltransferase -
  RX143_RS08575 (RX143_08575) - 1747275..1748255 (+) 981 WP_002378476.1 polyprenyl synthetase family protein -
  RX143_RS08580 (RX143_08580) cydC 1748335..1750101 (-) 1767 WP_025189868.1 thiol reductant ABC exporter subunit CydC -
  RX143_RS08585 (RX143_08585) cydD 1750098..1751831 (-) 1734 WP_010819498.1 thiol reductant ABC exporter subunit CydD -

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=890324 RX143_RS08520 WP_002356991.1 1733451..1733726(-) (comGC/cglC) [Enterococcus faecalis strain ES-3222]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=890324 RX143_RS08520 WP_002356991.1 1733451..1733726(-) (comGC/cglC) [Enterococcus faecalis strain ES-3222]
ATGAAAAAGAAACAAAAATACGCAGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTCCCTAACTTAGCGAAACATAAAGAAACAGTTGATAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCCTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396