Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RS899_RS01160 Genome accession   NZ_CP135919
Coordinates   249046..249456 (+) Length   136 a.a.
NCBI ID   WP_315808889.1    Uniprot ID   -
Organism   Pseudomonas sp. C9-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 249734..257890 249046..249456 flank 278


Gene organization within MGE regions


Location: 249046..257890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS899_RS01160 pilA 249046..249456 (+) 411 WP_315808889.1 pilin Machinery gene
  RS899_RS01165 - 249524..251413 (+) 1890 WP_315808890.1 hypothetical protein -
  RS899_RS01170 - 251410..252291 (+) 882 WP_315808891.1 glycosyltransferase family 2 protein -
  RS899_RS01175 - 252309..253412 (-) 1104 WP_315808892.1 glycosyltransferase family 4 protein -
  RS899_RS01180 - 253412..254530 (-) 1119 WP_315808893.1 glycosyltransferase -
  RS899_RS01185 - 254527..255294 (-) 768 WP_315808894.1 FkbM family methyltransferase -
  RS899_RS01190 - 255411..256688 (-) 1278 WP_315808895.1 oligosaccharide flippase family protein -
  RS899_RS01195 - 256685..257863 (-) 1179 WP_315808896.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 13713.77 Da        Isoelectric Point: 8.4971

>NTDB_id=887664 RS899_RS01160 WP_315808889.1 249046..249456(+) (pilA) [Pseudomonas sp. C9-3]
MKAQKGFTLIELMIVVAIIGILAAIAIPAYSNYQAKAKLAAGLAEASAGKTAFESKVNDGEDFTTPAAIGLTASTGNCTL
AATATAGAGSITCNIKNAPSQVKTAVITWARTASGDWSCKTTGVTDTTLLPKSCAN

Nucleotide


Download         Length: 411 bp        

>NTDB_id=887664 RS899_RS01160 WP_315808889.1 249046..249456(+) (pilA) [Pseudomonas sp. C9-3]
ATGAAGGCTCAAAAAGGCTTCACCCTGATCGAACTCATGATCGTGGTTGCAATCATCGGCATCCTGGCCGCCATCGCCAT
CCCGGCTTACAGCAACTATCAGGCTAAAGCCAAGCTGGCTGCTGGTCTGGCTGAGGCTTCCGCTGGTAAAACCGCTTTCG
AAAGCAAGGTGAACGACGGTGAGGACTTCACCACTCCGGCTGCGATCGGCCTGACCGCTTCTACTGGCAACTGCACTCTT
GCCGCGACTGCCACCGCTGGTGCCGGTTCCATCACTTGCAATATCAAGAACGCCCCATCCCAAGTTAAAACTGCTGTCAT
CACTTGGGCGCGCACTGCATCGGGTGATTGGTCCTGCAAGACCACTGGTGTAACCGATACCACACTGCTTCCGAAGTCTT
GCGCGAACTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-134)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

49.645

100

0.515
  comP Acinetobacter baylyi ADP1

45.333

100

0.5
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.183

100

0.493
  pilA Pseudomonas aeruginosa PAK

42.105

100

0.471
  pilA/pilAII Pseudomonas stutzeri DSM 10701

47.407

99.265

0.471
  pilA/pilA1 Eikenella corrodens VA1

36.42

100

0.434
  pilA Vibrio cholerae C6706

41.259

100

0.434
  pilA Vibrio cholerae strain A1552

41.259

100

0.434
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.259

100

0.434
  pilA2 Legionella pneumophila str. Paris

43.284

98.529

0.426
  pilA2 Legionella pneumophila strain ERS1305867

41.791

98.529

0.412