Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   RHM64_RS20410 Genome accession   NZ_CP133776
Coordinates   4530930..4532147 (+) Length   405 a.a.
NCBI ID   WP_322166094.1    Uniprot ID   -
Organism   Pseudomonas sp. AH2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4525930..4537147
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM64_RS20390 (RHM64_20390) nadC 4527420..4528268 (+) 849 WP_322166091.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RHM64_RS20400 (RHM64_20400) pilA2 4528581..4529003 (-) 423 WP_322166092.1 pilin Machinery gene
  RHM64_RS20405 (RHM64_20405) pilB 4529227..4530927 (+) 1701 WP_322166093.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RHM64_RS20410 (RHM64_20410) pilC 4530930..4532147 (+) 1218 WP_322166094.1 type II secretion system F family protein Machinery gene
  RHM64_RS20415 (RHM64_20415) pilD 4532149..4533021 (+) 873 WP_322166095.1 A24 family peptidase Machinery gene
  RHM64_RS20420 (RHM64_20420) coaE 4533018..4533641 (+) 624 WP_322166096.1 dephospho-CoA kinase -
  RHM64_RS20425 (RHM64_20425) yacG 4533638..4533841 (+) 204 WP_322166097.1 DNA gyrase inhibitor YacG -
  RHM64_RS20430 (RHM64_20430) - 4533869..4534567 (-) 699 WP_322166098.1 energy-coupling factor ABC transporter permease -
  RHM64_RS20435 (RHM64_20435) - 4534767..4535396 (+) 630 WP_322166099.1 DUF1780 domain-containing protein -
  RHM64_RS20440 (RHM64_20440) - 4535393..4535902 (+) 510 WP_322166100.1 MOSC domain-containing protein -
  RHM64_RS20445 (RHM64_20445) - 4535939..4536112 (+) 174 WP_322166101.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44242.27 Da        Isoelectric Point: 10.0118

>NTDB_id=876626 RHM64_RS20410 WP_322166094.1 4530930..4532147(+) (pilC) [Pseudomonas sp. AH2]
MATKAIKVSVYAWEGKDKKGVKLTGELNGHNPALVKALLRKQGITPVKVRKKTASFFAEGKKIKPIDIAFFTRQMSTMMK
AGVPLLQSFDIIGESADNPRMRTLVNEIKQEVAAGNSFASSLRKKPEYFDDLYCSLVDAGEQAGALEALLERVATYKEKT
EMLKAKIKKAMTYPIAVVIVALIVSAILLIKVVPQFQSVFSSFGAKLPTFTLMVIGLSELLQNWWYIVLAGVVLSIYCFK
RAYKTSQKFRDSLDRGLLKVPVVGPLIYKSSVARYARTLSTTFAAGVPLVEALESVASATGNVVFRNAVNRIKLDVSTGM
QLNFSMRSTGVFPTLAIQMAAIGEESGALDSMLDKVATFYEDEVDAMVDNMTALMEPFIMAVLGVVVGGLVIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=876626 RHM64_RS20410 WP_322166094.1 4530930..4532147(+) (pilC) [Pseudomonas sp. AH2]
ATGGCGACCAAGGCAATTAAAGTCAGTGTTTACGCCTGGGAAGGCAAGGATAAAAAGGGTGTAAAACTGACCGGCGAATT
AAATGGGCACAATCCCGCCTTGGTGAAAGCACTGTTGCGTAAGCAAGGCATCACACCCGTCAAAGTACGTAAAAAAACCG
CCTCGTTTTTTGCTGAAGGCAAAAAAATAAAGCCGATTGATATTGCGTTTTTCACGCGGCAGATGTCAACCATGATGAAA
GCAGGAGTTCCACTGCTGCAATCTTTCGACATCATCGGCGAAAGCGCTGATAACCCTCGAATGAGAACCTTAGTCAACGA
AATCAAACAAGAAGTCGCTGCAGGTAACAGCTTCGCGTCGTCCTTACGGAAAAAACCAGAATACTTCGATGATCTTTATT
GCAGCTTGGTTGACGCAGGCGAGCAAGCGGGTGCATTGGAAGCCTTGCTGGAACGGGTGGCGACCTACAAAGAAAAGACA
GAAATGCTCAAGGCCAAAATAAAGAAAGCCATGACTTATCCCATAGCGGTGGTAATAGTCGCCTTGATTGTTTCGGCCAT
TCTCCTAATAAAAGTTGTCCCTCAATTTCAGTCAGTTTTTTCAAGCTTTGGTGCAAAATTACCTACGTTTACGTTGATGG
TCATCGGTCTTTCAGAGCTGTTGCAGAATTGGTGGTATATCGTTTTAGCGGGAGTTGTTCTTTCAATCTATTGTTTTAAA
CGAGCCTACAAAACTTCCCAAAAATTCCGAGACTCGTTAGATCGCGGACTGCTTAAAGTCCCGGTTGTGGGCCCATTGAT
TTACAAATCATCGGTCGCCCGCTACGCCCGCACCCTATCCACCACTTTTGCGGCTGGTGTACCGCTTGTCGAAGCACTGG
AATCTGTCGCCAGCGCCACGGGGAATGTGGTGTTCAGAAACGCAGTGAACCGCATCAAACTCGACGTATCCACTGGCATG
CAGCTGAATTTTTCAATGCGCTCCACCGGAGTATTTCCAACGCTAGCGATTCAAATGGCTGCCATTGGTGAAGAGTCAGG
CGCCCTGGACTCAATGCTCGATAAAGTCGCAACCTTTTACGAAGACGAAGTTGACGCTATGGTCGATAACATGACCGCGC
TGATGGAGCCCTTCATCATGGCAGTCCTCGGCGTAGTAGTCGGTGGCCTCGTCATCGCGATGTACCTCCCCATCTTCCAG
CTCGGAAACGTCGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

76.79

100

0.768
  pilC Acinetobacter baylyi ADP1

58.68

100

0.593
  pilC Acinetobacter baumannii D1279779

58.519

100

0.585
  pilC Legionella pneumophila strain ERS1305867

51.515

97.778

0.504
  pilG Neisseria gonorrhoeae MS11

42.432

99.506

0.422
  pilG Neisseria meningitidis 44/76-A

42.184

99.506

0.42
  pilC Vibrio cholerae strain A1552

40.988

100

0.41
  pilC Vibrio campbellii strain DS40M4

40.64

100

0.407
  pilC Thermus thermophilus HB27

36.409

99.012

0.36