Detailed information NTG0876627

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: RHM64_RS20415
• Genome accession: NZ_CP133776
• Coordinates: 4532149..4533021 (+)
• Length: 290 a.a.
• NCBI ID: WP_322166095.1
• Uniprot ID: -
• Organism: Pseudomonas sp. AH2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4527149..4538021

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RHM64_RS20390 (RHM64_20390)	nadC	4527420..4528268 (+)	849	WP_322166091.1	carboxylating nicotinate-nucleotide diphosphorylase	-
RHM64_RS20400 (RHM64_20400)	pilA2	4528581..4529003 (-)	423	WP_322166092.1	pilin	Machinery gene
RHM64_RS20405 (RHM64_20405)	pilB	4529227..4530927 (+)	1701	WP_322166093.1	type IV-A pilus assembly ATPase PilB	Machinery gene
RHM64_RS20410 (RHM64_20410)	pilC	4530930..4532147 (+)	1218	WP_322166094.1	type II secretion system F family protein	Machinery gene
RHM64_RS20415 (RHM64_20415)	pilD	4532149..4533021 (+)	873	WP_322166095.1	A24 family peptidase	Machinery gene
RHM64_RS20420 (RHM64_20420)	coaE	4533018..4533641 (+)	624	WP_322166096.1	dephospho-CoA kinase	-
RHM64_RS20425 (RHM64_20425)	yacG	4533638..4533841 (+)	204	WP_322166097.1	DNA gyrase inhibitor YacG	-
RHM64_RS20430 (RHM64_20430)	-	4533869..4534567 (-)	699	WP_322166098.1	energy-coupling factor ABC transporter permease	-
RHM64_RS20435 (RHM64_20435)	-	4534767..4535396 (+)	630	WP_322166099.1	DUF1780 domain-containing protein	-
RHM64_RS20440 (RHM64_20440)	-	4535393..4535902 (+)	510	WP_322166100.1	MOSC domain-containing protein	-
RHM64_RS20445 (RHM64_20445)	-	4535939..4536112 (+)	174	WP_322166101.1	DUF3094 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31920.26 Da
• Isoelectric Point: 8.4831

>NTDB_id=876627 RHM64_RS20415 WP_322166095.1 4532149..4533021(+) (pilD) [Pseudomonas sp. AH2]
MPLINLLASSPLAFVICTLVLGLLVGSFLNVVIYRLPIMLDRDWKSQSREMLSLPAEPEPDTFNLMLPHSRCPRCAHKIR
AWENLPVISYLFLGGKCSGCKAPISKRYPIIELACGLLSAFIAWHFGFGWQAAAMLVLVWGLLAMSLIDADHQLLPDALV
LPLLWLGLIVNTFGLFTNLHDALWGAVAGYMSLWLVFWTFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLASLVG
AVLGLIMLRLRNASTSTPIPFGPYLAIAGWIALLWGGQITASYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=876627 RHM64_RS20415 WP_322166095.1 4532149..4533021(+) (pilD) [Pseudomonas sp. AH2]
ATGCCGCTCATTAATTTGCTGGCCAGCTCTCCCCTGGCCTTTGTCATCTGCACTCTGGTATTGGGCCTGCTGGTCGGCAG
CTTTCTAAACGTCGTCATCTACCGCCTGCCCATCATGCTCGACCGCGATTGGAAGTCTCAATCTCGCGAAATGCTTTCGC
TCCCGGCTGAGCCGGAACCTGACACCTTTAACCTGATGCTCCCCCACTCCCGCTGCCCGCGCTGTGCGCACAAGATCCGA
GCATGGGAGAACTTGCCGGTGATCAGTTATCTGTTCCTCGGCGGCAAGTGCTCCGGGTGTAAAGCGCCGATCAGTAAGCG
TTACCCTATCATCGAACTCGCCTGCGGGCTGCTCTCGGCGTTTATTGCCTGGCATTTCGGGTTCGGCTGGCAGGCGGCGG
CGATGTTAGTGCTGGTGTGGGGCCTGCTGGCGATGAGTTTGATTGATGCGGACCATCAACTGTTGCCCGACGCGTTGGTG
CTGCCACTGCTATGGCTGGGTTTGATCGTTAACACCTTTGGGCTGTTCACTAACTTGCACGATGCCTTGTGGGGCGCGGT
GGCCGGTTACATGAGCCTGTGGTTGGTGTTTTGGACGTTTAAGTTGGTGACCGGCAAGGAAGGCATGGGTTATGGCGACT
TTAAGTTGCTGGCGATGCTCGGTGCGTGGGGTGGCTGGCAGATACTTCCGCTGACGATTTTGCTGGCGTCACTGGTGGGT
GCGGTGTTGGGCCTGATTATGTTGCGGTTGCGCAACGCGAGCACCAGCACGCCGATCCCCTTCGGTCCTTATTTGGCGAT
TGCAGGCTGGATCGCGCTGCTCTGGGGTGGTCAAATAACCGCCTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	51.228	98.276	0.503
pilD	Vibrio campbellii strain DS40M4	51.636	94.828	0.49
pilD	Acinetobacter baumannii D1279779	51.095	94.483	0.483
pilD	Acinetobacter nosocomialis M2	50.73	94.483	0.479
pilD	Neisseria gonorrhoeae MS11	51.779	87.241	0.452