Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   RHM64_RS20400 Genome accession   NZ_CP133776
Coordinates   4528581..4529003 (-) Length   140 a.a.
NCBI ID   WP_322166092.1    Uniprot ID   -
Organism   Pseudomonas sp. AH2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4523581..4534003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM64_RS20380 (RHM64_20380) ampD 4524167..4524715 (-) 549 WP_322166089.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RHM64_RS20385 (RHM64_20385) - 4524846..4527089 (-) 2244 WP_322166090.1 DUF1631 domain-containing protein -
  RHM64_RS20390 (RHM64_20390) nadC 4527420..4528268 (+) 849 WP_322166091.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RHM64_RS20400 (RHM64_20400) pilA2 4528581..4529003 (-) 423 WP_322166092.1 pilin Machinery gene
  RHM64_RS20405 (RHM64_20405) pilB 4529227..4530927 (+) 1701 WP_322166093.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RHM64_RS20410 (RHM64_20410) pilC 4530930..4532147 (+) 1218 WP_322166094.1 type II secretion system F family protein Machinery gene
  RHM64_RS20415 (RHM64_20415) pilD 4532149..4533021 (+) 873 WP_322166095.1 A24 family peptidase Machinery gene
  RHM64_RS20420 (RHM64_20420) coaE 4533018..4533641 (+) 624 WP_322166096.1 dephospho-CoA kinase -
  RHM64_RS20425 (RHM64_20425) yacG 4533638..4533841 (+) 204 WP_322166097.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14110.46 Da        Isoelectric Point: 8.0978

>NTDB_id=876624 RHM64_RS20400 WP_322166092.1 4528581..4529003(-) (pilA2) [Pseudomonas sp. AH2]
MKLQKGFTLIELMIVVAIIGILAAVALPAYQNYTMRARYTEITSVADGYKTAVAMCISSLGSAANCASGTNDIPATTATT
YVASVAVAAGVILVTPTGTGSIPVAATYQLTPVVNTGSTTWTVAGGCLASTPILCKVSGT

Nucleotide


Download         Length: 423 bp        

>NTDB_id=876624 RHM64_RS20400 WP_322166092.1 4528581..4529003(-) (pilA2) [Pseudomonas sp. AH2]
ATGAAATTGCAAAAAGGCTTTACGCTGATCGAACTCATGATCGTAGTAGCTATTATTGGCATCTTGGCAGCGGTTGCACT
TCCTGCTTACCAGAATTACACTATGCGTGCTCGCTACACTGAAATAACATCGGTTGCAGACGGTTATAAAACTGCCGTGG
CAATGTGTATATCTTCGTTGGGTAGCGCTGCTAACTGTGCAAGTGGAACAAATGATATTCCCGCTACCACCGCCACTACC
TATGTCGCGAGTGTAGCGGTTGCTGCAGGCGTGATTCTGGTGACGCCTACGGGTACTGGATCTATTCCTGTCGCTGCAAC
TTATCAGTTGACTCCAGTGGTAAACACCGGGTCGACCACTTGGACTGTAGCTGGTGGATGTTTAGCTTCGACTCCGATTC
TTTGTAAGGTTTCGGGAACATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-124)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

46.622

100

0.493
  pilA2 Legionella pneumophila strain ERS1305867

45.946

100

0.486
  comP Acinetobacter baylyi ADP1

45.455

94.286

0.429
  pilA/pilA1 Eikenella corrodens VA1

42.647

97.143

0.414
  pilA Ralstonia pseudosolanacearum GMI1000

44.628

86.429

0.386
  pilA Glaesserella parasuis strain SC1401

41.6

89.286

0.371