Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RHM64_RS20405 Genome accession   NZ_CP133776
Coordinates   4529227..4530927 (+) Length   566 a.a.
NCBI ID   WP_322166093.1    Uniprot ID   -
Organism   Pseudomonas sp. AH2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4524227..4535927
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM64_RS20385 (RHM64_20385) - 4524846..4527089 (-) 2244 WP_322166090.1 DUF1631 domain-containing protein -
  RHM64_RS20390 (RHM64_20390) nadC 4527420..4528268 (+) 849 WP_322166091.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RHM64_RS20400 (RHM64_20400) pilA2 4528581..4529003 (-) 423 WP_322166092.1 pilin Machinery gene
  RHM64_RS20405 (RHM64_20405) pilB 4529227..4530927 (+) 1701 WP_322166093.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RHM64_RS20410 (RHM64_20410) pilC 4530930..4532147 (+) 1218 WP_322166094.1 type II secretion system F family protein Machinery gene
  RHM64_RS20415 (RHM64_20415) pilD 4532149..4533021 (+) 873 WP_322166095.1 A24 family peptidase Machinery gene
  RHM64_RS20420 (RHM64_20420) coaE 4533018..4533641 (+) 624 WP_322166096.1 dephospho-CoA kinase -
  RHM64_RS20425 (RHM64_20425) yacG 4533638..4533841 (+) 204 WP_322166097.1 DNA gyrase inhibitor YacG -
  RHM64_RS20430 (RHM64_20430) - 4533869..4534567 (-) 699 WP_322166098.1 energy-coupling factor ABC transporter permease -
  RHM64_RS20435 (RHM64_20435) - 4534767..4535396 (+) 630 WP_322166099.1 DUF1780 domain-containing protein -
  RHM64_RS20440 (RHM64_20440) - 4535393..4535902 (+) 510 WP_322166100.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62540.78 Da        Isoelectric Point: 5.7455

>NTDB_id=876625 RHM64_RS20405 WP_322166093.1 4529227..4530927(+) (pilB) [Pseudomonas sp. AH2]
MTDVALTGLAKQLAVAGLLNEKVAQDAYQKAQRSKISLVSYLVQNKLLKSIVLAEIASEQFGVPFLDLSTLDKEGQPKDL
VSEKLIRQHHVLPLWRRGNKLFVGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTDAIEKYFEGANAGLGEMADVDLEN
LDSDTLDDKKQDTIAGQDADDAPVVRFVNKMLLDAIKAGSSDLHFEPYEKTYRVRLRTDGILHEVAKPPVQLASRIAARL
KVMASLDISERRRPQDGRIKLRVSKTKAIDFRVNTLPTLWGEKIVMRILDPSTAQMGIDALGYEPDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKASQTGHMVLSTLHTNSAAETLTRLQHMGVAAFNIATAINLIIAQRLARKLCPLCKKEADIPRETLIREGFPE
DKIGTFKIYSPAGCDHCNNGYKGRVGIYEVVKNTPELQRLIMEEGNSMDIAVQMRKDGFNDLRTSGLMKAMQGLTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=876625 RHM64_RS20405 WP_322166093.1 4529227..4530927(+) (pilB) [Pseudomonas sp. AH2]
ATGACTGATGTCGCTTTAACAGGTTTGGCCAAGCAGCTGGCGGTAGCGGGGCTGCTAAACGAAAAAGTCGCTCAAGACGC
TTACCAAAAAGCTCAGCGCAGCAAAATATCGCTGGTCAGCTACCTGGTGCAGAATAAACTGCTTAAAAGCATCGTTTTGG
CGGAAATTGCTTCAGAGCAATTTGGTGTGCCCTTTCTAGACTTAAGCACGCTTGATAAAGAGGGACAGCCCAAAGACTTG
GTAAGCGAAAAATTGATTCGCCAACACCATGTCTTGCCGTTATGGCGACGGGGGAATAAATTATTTGTCGGCATTTCAGA
CCCTACCAACCACCAAGCAGTTACCGATATTCAGTTCAGCACGGGGCTTAACACTGAAGCGATCCTGGTAGAAGATGACA
AGTTAACTGACGCTATCGAAAAGTATTTTGAAGGCGCCAACGCCGGCTTGGGCGAAATGGCAGATGTTGATTTAGAGAAT
CTCGACTCTGACACCTTAGATGATAAGAAACAGGACACCATCGCAGGCCAAGACGCAGACGATGCGCCCGTGGTCCGTTT
CGTTAATAAGATGCTATTGGATGCCATTAAAGCAGGCTCTTCCGACCTCCACTTTGAACCTTATGAAAAAACCTATCGAG
TGCGCCTACGAACCGACGGTATTCTTCACGAGGTAGCAAAGCCGCCCGTTCAGTTGGCCAGCAGGATCGCTGCGCGCTTA
AAGGTCATGGCCAGCCTGGATATCTCAGAGCGCCGCCGTCCACAAGACGGCCGGATCAAACTGCGGGTTTCCAAAACCAA
GGCGATCGACTTTCGAGTCAATACCTTGCCCACCTTGTGGGGCGAAAAGATCGTGATGCGGATTCTTGATCCGTCCACCG
CACAGATGGGGATTGATGCGCTCGGCTATGAGCCCGATCAAAAAGAGCTGTATCTGGCTGCGCTTAAACAGCCCCAGGGT
ATGATTTTGGTTACCGGGCCCACCGGTTCAGGCAAAACCGTTTCGCTCTACACCGGCCTGAATATCCTTAACACCGTGGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGTATCAATCAGGTTAACGTCAACCCACGCCAAG
GCATGGACTTTTCGCAGGCACTGCGCGCCTTCTTGCGTCAGGACCCGGATGTAATCATGGTCGGGGAAATTCGCGACTTG
GAAACGGCGGAAATTGCCATCAAGGCCTCGCAAACAGGCCACATGGTGTTGTCTACGTTGCACACTAACAGCGCCGCGGA
AACCCTGACTCGCCTCCAGCACATGGGCGTGGCTGCATTCAACATTGCTACAGCTATTAACCTGATCATCGCTCAACGTT
TGGCACGCAAATTGTGTCCCTTGTGCAAGAAAGAAGCGGATATCCCCCGTGAGACGCTGATCCGCGAAGGTTTTCCCGAG
GACAAAATTGGCACGTTCAAAATTTACTCACCGGCCGGCTGTGACCATTGCAATAACGGCTATAAAGGCCGAGTCGGGAT
TTATGAGGTGGTGAAAAACACCCCCGAGTTGCAACGGCTCATCATGGAGGAAGGTAACTCCATGGATATCGCTGTTCAAA
TGCGTAAAGACGGCTTTAACGACCTGCGCACGTCGGGATTGATGAAGGCGATGCAAGGCCTTACCAGCCTCGAAGAAATC
AACCGTGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-144)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.821

100

0.581
  pilB Acinetobacter baylyi ADP1

57.368

100

0.578
  pilB Legionella pneumophila strain ERS1305867

55.477

100

0.555
  pilB Vibrio cholerae strain A1552

50.89

99.293

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

49.823

100

0.498
  pilB Vibrio campbellii strain DS40M4

49.468

99.647

0.493
  pilF Neisseria gonorrhoeae MS11

47.695

99.647

0.475
  pilF Thermus thermophilus HB27

37.655

99.47

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.49

95.936

0.369
  pilB/pilB1 Synechocystis sp. PCC 6803

39.306

91.696

0.36