Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MIN45_RS00340 Genome accession   NZ_AP024718
Coordinates   70158..71282 (+) Length   374 a.a.
NCBI ID   WP_286292635.1    Uniprot ID   A0AAU9BVT9
Organism   Methylomarinovum tepidoasis strain IN45     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 65158..76282
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIN45_RS00320 (MIN45_P0058) - 66146..68032 (+) 1887 WP_286292631.1 nucleoside-diphosphate sugar epimerase/dehydratase -
  MIN45_RS00325 (MIN45_P0059) - 68029..68376 (+) 348 WP_286292632.1 hypothetical protein -
  MIN45_RS00330 (MIN45_P0060) - 68381..69082 (-) 702 WP_286292633.1 YggS family pyridoxal phosphate-dependent enzyme -
  MIN45_RS00335 (MIN45_P0061) pilT 69111..70145 (+) 1035 WP_286292634.1 type IV pilus twitching motility protein PilT Machinery gene
  MIN45_RS00340 (MIN45_P0062) pilU 70158..71282 (+) 1125 WP_286292635.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MIN45_RS00345 (MIN45_P0063) - 71285..72559 (-) 1275 WP_286292636.1 dihydroorotase -
  MIN45_RS00350 (MIN45_P0064) - 72556..73515 (-) 960 WP_286292637.1 aspartate carbamoyltransferase catalytic subunit -
  MIN45_RS00355 (MIN45_P0065) pyrR 73512..74024 (-) 513 WP_286292638.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  MIN45_RS00360 (MIN45_P0066) ruvX 74021..74455 (-) 435 WP_286292639.1 Holliday junction resolvase RuvX -
  MIN45_RS00365 (MIN45_P0067) - 74448..75014 (-) 567 WP_286292640.1 YqgE/AlgH family protein -
  MIN45_RS00370 (MIN45_P0068) - 75026..75850 (-) 825 WP_286292641.1 energy transducer TonB -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 42205.47 Da        Isoelectric Point: 6.1384

>NTDB_id=87658 MIN45_RS00340 WP_286292635.1 70158..71282(+) (pilU) [Methylomarinovum tepidoasis strain IN45]
MNVESMLTLMVHKKASDLFVIAGRPPCIKVNGRLEPISPKSLSEPMVRELVLSTMDARQRDEFENARECNFAIAMENLAR
FRVSAYYQRNSPAMVIRRIQDYIPDVEELNLPPILKDLAMTKRGLIIFVGATGTGKSTSLAAMLKYRNHNSSGHIITIED
PIEYTHQHAGCIVTQREVGTDTESYEVALKNTLRQAPDVILIGEIRTRETMQHAITFAETGHLCLSTLHANNANQALDRI
LHFFPEDMHSQLFMDLSLNLKAIVAQQLIRCADGEGRYPAVEILINTPLASDLIRKGEVHKLKDLMKRSREQGMQTFDQC
LYDLYKAGKISYEDALAAADSRNEVRLMIKLGETGDLDRYAPGEDKMRLVEEDD

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=87658 MIN45_RS00340 WP_286292635.1 70158..71282(+) (pilU) [Methylomarinovum tepidoasis strain IN45]
ATGAACGTGGAATCCATGCTCACCCTGATGGTGCACAAGAAGGCCTCGGATCTGTTCGTGATCGCTGGCCGGCCGCCGTG
CATCAAGGTCAACGGCCGCCTGGAGCCCATCAGCCCCAAATCCCTCAGCGAACCCATGGTGCGCGAGCTGGTGCTCAGCA
CCATGGACGCGCGCCAGCGGGACGAGTTCGAAAACGCCCGCGAGTGCAACTTCGCCATCGCCATGGAGAACCTGGCCCGC
TTCCGCGTCAGCGCCTATTACCAGCGCAACAGCCCGGCGATGGTGATCCGGCGCATCCAGGATTACATCCCCGACGTGGA
GGAGCTGAACCTGCCACCGATCCTCAAGGATCTGGCGATGACCAAACGCGGTCTGATCATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCCCTGGCGGCGATGCTCAAATACCGCAACCACAACAGCTCCGGGCACATCATCACCATCGAGGAT
CCGATCGAGTACACCCACCAGCACGCCGGCTGCATCGTCACCCAGCGCGAGGTGGGCACCGACACCGAATCCTACGAGGT
GGCCCTGAAGAACACCCTGCGCCAGGCCCCGGACGTGATCCTCATCGGCGAGATCCGCACCCGGGAGACCATGCAGCACG
CCATCACCTTCGCCGAAACCGGGCATCTGTGCCTGTCCACCCTGCACGCCAACAACGCCAACCAGGCTCTGGACCGCATC
CTGCACTTCTTCCCCGAGGACATGCACAGCCAGCTGTTCATGGACCTGTCCCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGATCCGCTGCGCCGACGGCGAGGGCCGCTATCCGGCGGTGGAGATCCTCATCAACACCCCGCTGGCCTCGGACCTGA
TCCGCAAGGGCGAGGTCCACAAGCTCAAGGACTTGATGAAGCGCTCGCGCGAACAGGGGATGCAGACCTTCGACCAGTGC
CTCTACGATCTCTACAAGGCCGGCAAGATCAGCTACGAGGACGCCCTGGCCGCCGCCGACTCGCGCAACGAGGTGCGGCT
GATGATCAAACTGGGGGAAACGGGCGACCTCGACCGGTACGCGCCCGGCGAAGACAAGATGCGGCTGGTGGAGGAGGACG
ACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.241

100

0.652
  pilU Acinetobacter baylyi ADP1

60.753

99.465

0.604
  pilU Vibrio cholerae strain A1552

56.198

97.059

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.954

93.048

0.39
  pilT Legionella pneumophila strain Lp02

42.478

90.642

0.385
  pilT Legionella pneumophila strain ERS1305867

42.478

90.642

0.385
  pilT Acinetobacter nosocomialis M2

40.118

90.642

0.364
  pilT Acinetobacter baumannii D1279779

40.118

90.642

0.364
  pilT Acinetobacter baumannii strain A118

40.118

90.642

0.364
  pilT Acinetobacter baylyi ADP1

39.589

91.176

0.361

Multiple sequence alignment