Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MIN45_RS00335 Genome accession   NZ_AP024718
Coordinates   69111..70145 (+) Length   344 a.a.
NCBI ID   WP_286292634.1    Uniprot ID   A0AAU9C3Q5
Organism   Methylomarinovum tepidoasis strain IN45     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 64111..75145
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIN45_RS00310 (MIN45_P0056) flgA 64169..64864 (-) 696 WP_286292629.1 flagellar basal body P-ring formation chaperone FlgA -
  MIN45_RS00315 (MIN45_P0057) - 65072..65932 (-) 861 WP_286292630.1 porin family protein -
  MIN45_RS00320 (MIN45_P0058) - 66146..68032 (+) 1887 WP_286292631.1 nucleoside-diphosphate sugar epimerase/dehydratase -
  MIN45_RS00325 (MIN45_P0059) - 68029..68376 (+) 348 WP_286292632.1 hypothetical protein -
  MIN45_RS00330 (MIN45_P0060) - 68381..69082 (-) 702 WP_286292633.1 YggS family pyridoxal phosphate-dependent enzyme -
  MIN45_RS00335 (MIN45_P0061) pilT 69111..70145 (+) 1035 WP_286292634.1 type IV pilus twitching motility protein PilT Machinery gene
  MIN45_RS00340 (MIN45_P0062) pilU 70158..71282 (+) 1125 WP_286292635.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MIN45_RS00345 (MIN45_P0063) - 71285..72559 (-) 1275 WP_286292636.1 dihydroorotase -
  MIN45_RS00350 (MIN45_P0064) - 72556..73515 (-) 960 WP_286292637.1 aspartate carbamoyltransferase catalytic subunit -
  MIN45_RS00355 (MIN45_P0065) pyrR 73512..74024 (-) 513 WP_286292638.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  MIN45_RS00360 (MIN45_P0066) ruvX 74021..74455 (-) 435 WP_286292639.1 Holliday junction resolvase RuvX -
  MIN45_RS00365 (MIN45_P0067) - 74448..75014 (-) 567 WP_286292640.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38241.87 Da        Isoelectric Point: 7.0357

>NTDB_id=87657 MIN45_RS00335 WP_286292634.1 69111..70145(+) (pilT) [Methylomarinovum tepidoasis strain IN45]
MDIAELLAFSVKNNASDLHLSAGLPPMIRVDGDIRRINVPPLEHKEVHALVYDIMNDKQRRDYEEYLETDFSFELPGVAR
FRVNAFHQDRGAAAVFRTIPSKVLTLEDLGCPPFFRDVTQKPRGLILVTGPTGSGKSTTLAAMIDYINSNNYSHILTVED
PIEFVHQSKKSLINQREVHRDTLGFNEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKAMIRSMLSESLQAVIAQTLLKKIGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGRKDGMQTLDQHL
QELVDKGVISRQTARTKAVNKAAF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=87657 MIN45_RS00335 WP_286292634.1 69111..70145(+) (pilT) [Methylomarinovum tepidoasis strain IN45]
ATGGACATCGCCGAACTGCTCGCCTTTTCCGTCAAGAACAACGCCTCCGACCTCCACCTCTCCGCCGGCCTGCCACCGAT
GATTCGCGTTGACGGCGACATCCGCCGCATCAACGTGCCGCCGCTGGAGCACAAGGAGGTCCACGCGCTAGTTTACGACA
TCATGAACGACAAGCAGCGGCGCGATTACGAGGAATATCTGGAAACCGACTTTTCCTTCGAGCTGCCCGGAGTGGCCCGT
TTCCGCGTCAACGCTTTCCATCAGGACCGGGGGGCGGCGGCGGTGTTCCGCACCATTCCCTCCAAGGTACTGACCCTGGA
GGATCTGGGCTGCCCACCCTTCTTCCGTGACGTGACCCAGAAACCCCGCGGCCTGATTCTGGTCACCGGCCCCACCGGCT
CCGGTAAGTCCACCACCCTGGCGGCGATGATCGACTACATCAATTCCAACAATTACTCCCATATTCTCACCGTCGAGGAC
CCGATCGAATTCGTCCATCAGAGCAAGAAATCCCTCATCAACCAGCGCGAGGTCCACCGCGACACCCTCGGCTTCAACGA
AGCCTTGAGATCGGCGCTGCGCGAGGACCCGGACGTGATCCTGGTGGGGGAAATGCGCGATCTTGAAACCATCCGCCTGG
CCCTGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACTCTGCACACCTCCTCGGCGGCCAAAACCATCGACCGTATC
ATCGACGTGTTCCCGGCGGCGGAAAAGGCCATGATCCGCTCGATGCTGTCGGAATCCCTGCAGGCGGTCATCGCCCAGAC
CCTGCTGAAGAAGATCGGCGGCGGGCGGACGGCCGCCTGGGAGATCATGGTCGGGACCCCGGCGATCCGCAACCTGATCC
GTGAGGACAAAGTGGCGCAGATGTACTCGGCGATCCAGACCGGACGCAAGGACGGCATGCAGACCCTCGACCAGCACCTG
CAGGAACTGGTGGACAAAGGCGTCATCTCGCGCCAGACCGCCCGCACCAAGGCCGTCAACAAGGCGGCGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

81.686

100

0.817
  pilT Acinetobacter baumannii D1279779

81.686

100

0.817
  pilT Acinetobacter baumannii strain A118

81.686

100

0.817
  pilT Pseudomonas aeruginosa PAK

81.657

98.256

0.802
  pilT Pseudomonas stutzeri DSM 10701

79.07

100

0.791
  pilT Acinetobacter baylyi ADP1

78.488

100

0.785
  pilT Legionella pneumophila strain Lp02

76.453

100

0.765
  pilT Legionella pneumophila strain ERS1305867

76.453

100

0.765
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.03

97.965

0.686
  pilT Vibrio cholerae strain A1552

70.03

97.965

0.686
  pilT Neisseria meningitidis 8013

66.957

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

40.299

97.384

0.392
  pilU Acinetobacter baylyi ADP1

40.06

96.512

0.387

Multiple sequence alignment