Detailed information NTG0871823

Bioinformatically predicted

Overview

• Name: pilQ
• Type: Machinery gene
• Locus tag: RBU63_RS24270
• Genome accession: NZ_CP133092
• Coordinates: 5264942..5266147 (-)
• Length: 401 a.a.
• NCBI ID: WP_088852014.1
• Uniprot ID: -
• Organism: Pseudomonas mosselii strain JP2-207
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	5267114..5278028	5264942..5266147	flank	967

Gene organization within MGE regions

Location: 5264942..5278028

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RBU63_RS24270	pilQ	5264942..5266147 (-)	1206	WP_088852014.1	type IV pilus secretin PilQ	Machinery gene
RBU63_RS24275	-	5266104..5267117 (-)	1014	WP_051555621.1	pilus assembly protein PilP	-
RBU63_RS24280	-	5267114..5267653 (-)	540	WP_307943179.1	PilN domain-containing protein	-
RBU63_RS24285	pilM	5267650..5268546 (-)	897	WP_028689112.1	type IV pilus biogenesis protein PilM	-
RBU63_RS24290	-	5268716..5271166 (+)	2451	WP_371349763.1	penicillin-binding protein 1A	-
RBU63_RS24295	-	5271261..5272529 (-)	1269	WP_028689114.1	malic enzyme-like NAD(P)-binding protein	-
RBU63_RS24300	-	5272709..5273530 (-)	822	WP_028689115.1	thermonuclease family protein	-
RBU63_RS24305	rpmE	5273533..5273748 (-)	216	WP_011531746.1	50S ribosomal protein L31	-
RBU63_RS24310	-	5273918..5276137 (+)	2220	WP_062362830.1	primosomal protein N'	-
RBU63_RS24315	argS	5276292..5278028 (+)	1737	WP_307943182.1	arginine--tRNA ligase	-

Sequence

Protein

• Length: 401 a.a.
• Molecular weight: 43437.87 Da
• Isoelectric Point: 4.9947

>NTDB_id=871823 RBU63_RS24270 WP_088852014.1 5264942..5266147(-) (pilQ) [Pseudomonas mosselii strain JP2-207]
MLGLQATQAETFQGEPMSLNLQDVELRSVLQVMADHAGVNLVVGDSVQGRITLRLEDVPWEQALDLVLRSKGLGRRREGN
VLLVAPVAELAGQVQGIEPLQRELIPVFHAKAGELAELLLASLADDGALAGRGSLSVDARTNTLVATQPAARLAELRRLV
TQLDVPVRQVMIEARIVEANVDYEKALGVRWGGPLYGSDRRLGSDLFVDLGVERGGAALGVGLLRSDILLDLELSAMEKS
GNGEIISQPKVVTADKETARILKGTEVPYQETTRSGATSVSFREASLSLEVTPQITPDGKVIMAVRVTKDEPDFINALND
VPPIRKNEVNAKVRVTDGQTIVIGGVYSTSQNKVVDKVPFFGDLPYVGRLFRRDALQEKKSELLVFLTPRIMSDQAIAVS
R

Nucleotide

• Length: 1206 bp

>NTDB_id=871823 RBU63_RS24270 WP_088852014.1 5264942..5266147(-) (pilQ) [Pseudomonas mosselii strain JP2-207]
ATGTTGGGGCTTCAGGCGACGCAGGCCGAGACGTTTCAGGGTGAGCCGATGTCGCTCAACCTCCAGGACGTCGAGCTGCG
TTCGGTGCTTCAGGTCATGGCCGATCACGCGGGCGTCAACCTGGTGGTCGGCGACTCGGTGCAGGGACGGATTACCCTGC
GCCTGGAAGACGTGCCCTGGGAGCAGGCGCTTGATCTGGTGCTGCGCAGCAAGGGCTTGGGCAGGCGCCGGGAGGGGAAT
GTGCTGCTGGTTGCTCCTGTGGCAGAGCTCGCTGGGCAGGTCCAGGGCATCGAGCCACTGCAGCGTGAATTGATCCCTGT
CTTCCATGCCAAGGCCGGCGAGCTGGCGGAGCTGCTGCTGGCGAGCCTGGCGGACGACGGTGCCCTGGCCGGGCGTGGCA
GCCTCAGTGTGGACGCTCGCACCAATACCCTGGTAGCCACCCAGCCTGCCGCGCGGCTCGCCGAACTGCGCAGGCTGGTC
ACTCAGCTGGATGTGCCGGTGCGCCAGGTCATGATCGAGGCACGGATCGTCGAGGCCAATGTCGATTATGAAAAGGCCCT
GGGCGTGCGCTGGGGTGGACCGCTGTATGGCTCGGACCGCCGGCTGGGCAGCGACCTTTTCGTCGATCTTGGCGTCGAGC
GGGGAGGTGCCGCGTTGGGAGTGGGGCTGCTGCGCAGCGATATCCTGCTCGATCTGGAGCTCAGCGCCATGGAAAAAAGC
GGCAATGGCGAGATCATCTCCCAACCCAAGGTGGTCACGGCCGACAAGGAAACCGCGCGCATACTCAAGGGCACCGAAGT
GCCCTACCAGGAAACCACCCGCAGTGGCGCCACTTCGGTCTCCTTCCGAGAGGCGTCCCTGTCGCTGGAGGTGACGCCGC
AGATCACGCCGGACGGCAAGGTGATCATGGCGGTGCGGGTGACCAAGGACGAGCCGGACTTCATCAATGCCCTTAACGAT
GTGCCGCCGATCCGCAAGAACGAGGTCAACGCCAAGGTGCGGGTCACCGATGGGCAGACCATTGTGATTGGTGGCGTGTA
CTCGACGTCGCAGAACAAAGTGGTCGACAAGGTGCCATTTTTTGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCG
ATGCATTACAAGAGAAAAAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGT
CGTTGA

Domains

Predicted by InterproScan.

• Secretin and TonB N terminus short domain (STN) (39-86)

• Bacterial type II/III secretion system short domain (Secretin_N) (104-169)

• Bacterial type II and III secretion system protein (Secretin) (236-392)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilQ	Pseudomonas aeruginosa PAK	57.11	100	0.621
comQ	Acinetobacter baylyi ADP1	42.163	100	0.476
pilQ	Acinetobacter baumannii D1279779	41.087	100	0.471
pilQ	Acinetobacter baumannii strain A118	41.087	100	0.471
pilQ	Legionella pneumophila strain ERS1305867	43.365	100	0.456
pilQ	Legionella pneumophila strain Lp02	43.365	100	0.456
pilQ	Vibrio campbellii strain DS40M4	40.094	100	0.424
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	40.144	100	0.416
pilQ	Vibrio cholerae strain A1552	40.144	100	0.416
pilQ	Neisseria meningitidis M1080	35.461	100	0.374
pilQ	Neisseria gonorrhoeae strain FA1090	35.071	100	0.369
pilQ	Neisseria meningitidis MC58	34.834	100	0.367