Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Q9G97_RS01740 Genome accession   NZ_CP132194
Coordinates   397074..398201 (-) Length   375 a.a.
NCBI ID   WP_305899485.1    Uniprot ID   -
Organism   Psychrobacter sp. M13     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 392074..403201
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q9G97_RS01710 (Q9G97_01710) - 392419..392754 (+) 336 WP_201571353.1 c-type cytochrome -
  Q9G97_RS01715 (Q9G97_01715) - 393050..393718 (+) 669 WP_201571354.1 cytochrome c -
  Q9G97_RS01720 (Q9G97_01720) - 393918..395048 (+) 1131 WP_305899482.1 hypothetical protein -
  Q9G97_RS01725 (Q9G97_01725) - 395048..395395 (+) 348 WP_201571356.1 RnfH family protein -
  Q9G97_RS01730 (Q9G97_01730) - 395435..395848 (-) 414 WP_305899483.1 outer membrane protein assembly factor BamE -
  Q9G97_RS01735 (Q9G97_01735) fur 396211..396642 (+) 432 WP_305899484.1 ferric iron uptake transcriptional regulator -
  Q9G97_RS01740 (Q9G97_01740) pilU 397074..398201 (-) 1128 WP_305899485.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q9G97_RS01745 (Q9G97_01745) pilT 398292..399329 (-) 1038 WP_305900247.1 type IV pilus twitching motility protein PilT Machinery gene
  Q9G97_RS01750 (Q9G97_01750) - 399571..400341 (+) 771 WP_371747919.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q9G97_RS01755 (Q9G97_01755) - 400378..401652 (-) 1275 WP_305899487.1 hemolysin family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 42161.27 Da        Isoelectric Point: 6.2334

>NTDB_id=867540 Q9G97_RS01740 WP_305899485.1 397074..398201(-) (pilU) [Psychrobacter sp. M13]
MDFDKLLQLMIKKNGSDLFITADVAPSMKMNGVIHPVGKTPLTIKQVDTLVKGVMTPAQRKEFSETNECQFAITDSAELA
RFRVSAFVQRDMPGMVLRKIENKIPTVDELQLPPLLKELAMKNRGIILLVGATGTGKSTTLAAMVGHRNQNSHGHIITIE
DPIEFIHQHQGCIITQREVGIDTLSFESGLKNTLRQAPNVILIGEIRNREVMNYAIQYAETGHLVFATLHANNANQAIDR
IIHFFEADRHSQLFMDLSLNLQAIIAQQLIPTPDGKGRRAAIEILLNSQLISDYIRKGEVHEIKEVMKRSRNNGMQTFDQ
ALFDLYENNEVTYEDAMIYADSPNDLRLQIKLNSKHSSSAQDQDQDLDNLSLDTF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=867540 Q9G97_RS01740 WP_305899485.1 397074..398201(-) (pilU) [Psychrobacter sp. M13]
ATGGACTTCGATAAGTTATTACAGTTAATGATCAAAAAAAATGGCTCTGACTTATTTATCACAGCTGACGTCGCGCCTTC
AATGAAAATGAACGGGGTTATCCATCCCGTGGGTAAAACGCCATTGACTATCAAGCAAGTCGATACTTTGGTAAAAGGGG
TGATGACGCCTGCGCAACGAAAAGAATTCTCTGAGACTAATGAATGTCAGTTCGCCATCACTGATAGTGCTGAATTAGCC
CGTTTTCGCGTTAGCGCCTTTGTGCAACGCGATATGCCAGGAATGGTCCTACGTAAAATTGAGAATAAAATTCCGACTGT
TGATGAGCTGCAATTACCACCTCTATTAAAAGAGCTGGCTATGAAAAACCGTGGCATCATCCTATTAGTTGGCGCGACTG
GTACAGGTAAGTCGACTACCCTTGCTGCTATGGTTGGGCATCGCAATCAAAACTCGCATGGTCATATTATTACTATTGAA
GATCCAATAGAGTTTATCCATCAGCATCAAGGCTGCATTATCACCCAGCGCGAAGTGGGTATCGATACCTTATCGTTTGA
GTCTGGCCTCAAAAATACCCTTCGCCAAGCACCAAATGTTATTTTGATTGGTGAAATTCGTAATCGTGAAGTGATGAACT
ACGCTATTCAATATGCTGAGACCGGCCATTTAGTTTTTGCAACTTTACACGCCAATAATGCCAACCAAGCTATTGACCGT
ATCATTCACTTTTTTGAAGCCGATCGTCATAGCCAGCTATTCATGGATTTATCGCTAAACCTGCAAGCTATCATTGCTCA
GCAGCTTATCCCTACCCCTGATGGTAAAGGGCGTCGCGCCGCTATTGAGATTTTATTAAACTCACAACTGATCAGTGACT
ATATCCGTAAAGGTGAGGTGCATGAGATCAAAGAGGTAATGAAGCGCTCACGGAACAACGGCATGCAGACCTTTGATCAG
GCACTGTTTGATTTATATGAAAATAATGAAGTCACTTATGAAGATGCCATGATATATGCAGACTCCCCTAATGATTTGCG
CCTGCAAATCAAGCTAAACAGTAAGCATAGCAGTAGTGCTCAGGATCAAGATCAAGACTTGGATAACCTATCCTTAGACA
CTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

68.451

94.667

0.648
  pilU Pseudomonas stutzeri DSM 10701

67.324

94.667

0.637
  pilU Vibrio cholerae strain A1552

52.422

93.6

0.491
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.04

92.267

0.379
  pilT Legionella pneumophila strain ERS1305867

41.667

89.6

0.373
  pilT Legionella pneumophila strain Lp02

41.667

89.6

0.373
  pilT Acinetobacter nosocomialis M2

39.65

91.467

0.363
  pilT Acinetobacter baumannii D1279779

39.65

91.467

0.363
  pilT Acinetobacter baumannii strain A118

39.65

91.467

0.363
  pilT Pseudomonas aeruginosa PAK

39.306

92.267

0.363