Detailed information NTG0862265

Bioinformatically predicted

Overview

• Name: pilE
• Type: Machinery gene
• Locus tag: WCU05_RS26360
• Genome accession: NZ_CP147622
• Coordinates: 5597235..5597708 (-)
• Length: 157 a.a.
• NCBI ID: WP_015503553.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain GN06251
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5592235..5602708

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WCU05_RS26325 (WCU05_26325)	-	5592715..5593377 (+)	663	WP_023082023.1	DUF3218 family protein	-
WCU05_RS26330 (WCU05_26330)	fliP	5593660..5594412 (+)	753	WP_023082025.1	flagellar type III secretion system pore protein FliP	-
WCU05_RS26335 (WCU05_26335)	fliQ	5594457..5594726 (+)	270	WP_023081841.1	flagellar biosynthesis protein FliQ	-
WCU05_RS26340 (WCU05_26340)	fliR	5594726..5595502 (+)	777	WP_003083054.1	flagellar biosynthetic protein FliR	-
WCU05_RS26345 (WCU05_26345)	-	5595505..5595764 (+)	260	Protein_5212	EscU/YscU/HrcU family type III secretion system export apparatus switch protein	-
WCU05_RS26350 (WCU05_26350)	-	5596762..5597004 (+)	243	WP_023082027.1	hypothetical protein	-
WCU05_RS26360 (WCU05_26360)	pilE	5597235..5597708 (-)	474	WP_015503553.1	pilin	Machinery gene
WCU05_RS26365 (WCU05_26365)	pilB	5597939..5599639 (+)	1701	WP_003120945.1	type IV-A pilus assembly ATPase PilB	Machinery gene
WCU05_RS26370 (WCU05_26370)	pilC	5599643..5600860 (+)	1218	WP_010792214.1	type II secretion system F family protein	Machinery gene
WCU05_RS26375 (WCU05_26375)	pilD	5600863..5601735 (+)	873	WP_003116251.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
WCU05_RS26380 (WCU05_26380)	coaE	5601732..5602343 (+)	612	WP_003112838.1	dephospho-CoA kinase	-
WCU05_RS26385 (WCU05_26385)	yacG	5602340..5602540 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

• Length: 157 a.a.
• Molecular weight: 16090.45 Da
• Isoelectric Point: 8.4684

>NTDB_id=862265 WCU05_RS26360 WP_015503553.1 5597235..5597708(-) (pilE) [Pseudomonas aeruginosa strain GN06251]
MKAAQKGFTLIELMIVVAIIGILAAIAIPAYQDYTARAQLSEAMTLASGLKTKVSDIFSQDGSCPANTAATAGIEKDTDI
NGKYVAKVTTGGTAAASGGCTIVATMKASDVATPLRGKTLTLTLGNADKGSYTWACTSNADNKYLPKTCQTATTTTP

Nucleotide

• Length: 474 bp

>NTDB_id=862265 WCU05_RS26360 WP_015503553.1 5597235..5597708(-) (pilE) [Pseudomonas aeruginosa strain GN06251]
ATGAAAGCTGCTCAAAAAGGCTTTACCTTGATCGAATTGATGATCGTGGTCGCGATCATCGGTATTCTGGCTGCCATCGC
TATTCCAGCGTATCAGGACTACACCGCACGCGCTCAGCTTAGCGAAGCCATGACCCTGGCCAGTGGTCTCAAGACGAAAG
TGAGCGATATCTTCTCTCAGGATGGGTCCTGCCCGGCTAATACTGCTGCCACGGCAGGCATCGAGAAAGATACCGACATC
AACGGCAAGTATGTTGCCAAGGTAACAACTGGTGGCACCGCAGCTGCGTCTGGTGGTTGCACTATCGTTGCTACTATGAA
AGCCTCTGATGTGGCTACTCCTCTGAGGGGGAAAACTCTGACTTTGACTCTAGGAAATGCTGACAAGGGTTCTTACACTT
GGGCCTGTACTTCCAACGCAGATAACAAGTACCTGCCAAAAACCTGCCAGACTGCTACCACTACCACTCCGTAA

Domains

Predicted by InterProScan.

• Pilin (bacterial filament) (Pilin) (36-150)

• Prokaryotic N-terminal methylation motif (N_methyl) (4-27)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilE	Neisseria gonorrhoeae strain FA1090	42.197	100	0.465
comP	Acinetobacter baylyi ADP1	47.059	97.452	0.459
pilE	Neisseria gonorrhoeae MS11	42.515	100	0.452
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	41.176	100	0.446
pilA/pilA1	Eikenella corrodens VA1	41.975	100	0.433
pilA2	Legionella pneumophila str. Paris	45.578	93.631	0.427
pilA2	Legionella pneumophila strain ERS1305867	44.218	93.631	0.414
pilA	Ralstonia pseudosolanacearum GMI1000	39.394	100	0.414
pilA/pilAI	Pseudomonas stutzeri DSM 10701	43.333	95.541	0.414
pilA	Pseudomonas aeruginosa PAK	41.722	96.178	0.401
pilA	Acinetobacter nosocomialis M2	55.357	71.338	0.395