Detailed information NTG0854838

Bioinformatically predicted

Overview

• Name: comGA
• Type: Machinery gene
• Locus tag: LLWG2_RS11535
• Genome accession: NZ_CP129660
• Coordinates: 2223304..2224284 (-)
• Length: 326 a.a.
• NCBI ID: WP_021164974.1
• Uniprot ID: O86279
• Organism: Lactococcus cremoris strain Wg2
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
ICE	2216054..2224284	2223304..2224284	within	0

Gene organization within MGE regions

Location: 2216054..2224284

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLWG2_RS11485 (LLWG2_11540)	-	2217520..2218329 (-)	810	WP_011677177.1	metal ABC transporter permease	-
LLWG2_RS11490 (LLWG2_11545)	-	2218322..2219059 (-)	738	WP_011677178.1	metal ABC transporter ATP-binding protein	-
LLWG2_RS11495 (LLWG2_11550)	-	2219238..2220080 (-)	843	WP_021164979.1	metal ABC transporter solute-binding protein, Zn/Mn family	-
LLWG2_RS11500 (LLWG2_11555)	-	2220077..2220514 (-)	438	WP_011677180.1	zinc-dependent MarR family transcriptional regulator	-
LLWG2_RS11505 (LLWG2_11560)	comGG	2220594..2220821 (-)	228	WP_228764408.1	competence protein ComGG	Machinery gene
LLWG2_RS11510 (LLWG2_11565)	comGF	2220917..2221342 (-)	426	WP_014735174.1	competence type IV pilus minor pilin ComGF	Machinery gene
LLWG2_RS11515 (LLWG2_11570)	comGE	2221326..2221622 (-)	297	WP_021164977.1	competence type IV pilus minor pilin ComGE	Machinery gene
LLWG2_RS11520 (LLWG2_11575)	comGD	2221594..2221782 (-)	189	WP_014573336.1	hypothetical protein	Machinery gene
LLWG2_RS11525 (LLWG2_11580)	comGC	2221984..2222334 (-)	351	WP_050574187.1	competence type IV pilus major pilin ComGC	Machinery gene
LLWG2_RS11530 (LLWG2_11585)	comGB	2222379..2223404 (-)	1026	WP_050574185.1	competence type IV pilus assembly protein ComGB	Machinery gene
LLWG2_RS11535 (LLWG2_11590)	comGA	2223304..2224284 (-)	981	WP_021164974.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 37057.37 Da
• Isoelectric Point: 5.7372

>NTDB_id=854838 LLWG2_RS11535 WP_021164974.1 2223304..2224284(-) (comGA) [Lactococcus cremoris strain Wg2]
MVQKKAQELIQKAIEMEASDIYLIASGNLYKIYIRQQLGRTLIEELNQEIGLALLTHFKFLAGMNTGERRRVQLSACWYE
LEGSSAKRLRLSTVGDFEGNESLVIRLLHDQKQELEFWFDDKLQDFRCKRGLYLFSGPVGSGKTSLMFDLAPRHFSNAQV
ITIEEPVELIESDFIQLQVNDVIGNSYDELIKLSLRHRPDLLIVGEIRDQQTARAVLRASLTGYTFFSTIHAASVKGVVQ
RLLELGLSDWELKNGLEAVVYQRLIAGKGVLDIAKSKFDTWSPKKWNEKIENLYANGHLTAIEAEREKISINQASKIDTT
DGESFE

Nucleotide

• Length: 981 bp

>NTDB_id=854838 LLWG2_RS11535 WP_021164974.1 2223304..2224284(-) (comGA) [Lactococcus cremoris strain Wg2]
ATGGTACAGAAAAAAGCACAAGAACTCATTCAAAAGGCAATTGAGATGGAGGCTTCTGATATTTATTTAATTGCTTCAGG
AAATCTTTATAAGATATATATTCGTCAACAATTAGGGCGAACTTTAATAGAGGAACTTAACCAAGAGATTGGTTTAGCAT
TACTTACTCATTTTAAATTTCTTGCTGGCATGAATACTGGTGAACGCCGGCGTGTTCAGCTGAGTGCTTGTTGGTATGAA
CTAGAGGGAAGTTCGGCAAAACGTTTGCGCCTTTCAACAGTGGGAGATTTTGAAGGAAATGAATCATTGGTTATCCGTCT
TTTGCATGACCAAAAACAAGAACTTGAGTTTTGGTTTGACGATAAACTTCAGGATTTTCGATGTAAACGAGGACTTTATT
TATTTTCTGGGCCAGTAGGGTCTGGGAAAACTTCACTAATGTTTGACTTAGCCCCCCGTCATTTTTCAAATGCACAGGTT
ATCACTATTGAGGAGCCTGTAGAATTAATTGAATCTGATTTTATTCAGTTACAAGTTAATGATGTAATTGGGAATAGTTA
TGATGAATTAATTAAACTTTCTTTAAGACACCGACCAGATTTACTAATTGTTGGAGAAATTCGAGATCAGCAGACAGCTC
GTGCTGTATTGCGTGCAAGTTTAACAGGCTATACATTTTTTTCTACTATCCATGCGGCTTCCGTGAAGGGAGTGGTTCAA
CGTTTGTTGGAATTGGGTTTGAGTGATTGGGAATTGAAAAATGGATTAGAGGCAGTTGTTTATCAACGGTTAATAGCGGG
AAAAGGAGTATTAGATATTGCGAAAAGTAAATTTGACACTTGGTCGCCGAAAAAATGGAATGAAAAGATTGAGAACTTAT
ATGCAAACGGACATCTTACGGCTATTGAGGCCGAAAGGGAAAAAATTAGCATTAATCAAGCAAGCAAAATTGATACAACT
GATGGGGAATCTTTTGAATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (9-265)

3D structure

• AlphaFold DB: O86279

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGA	Lactococcus lactis subsp. cremoris KW2	98.16	100	0.982
comGA/cglA	Streptococcus sobrinus strain NIDR 6715-7	55.806	95.092	0.531
comYA	Streptococcus gordonii str. Challis substr. CH1	53.797	96.933	0.521
comGA/cglA/cilD	Streptococcus pneumoniae R6	53.846	95.706	0.515
comGA/cglA/cilD	Streptococcus mitis NCTC 12261	53.846	95.706	0.515
comGA/cglA/cilD	Streptococcus pneumoniae TIGR4	53.846	95.706	0.515
comGA/cglA/cilD	Streptococcus pneumoniae Rx1	53.846	95.706	0.515
comGA/cglA/cilD	Streptococcus pneumoniae D39	53.846	95.706	0.515
comYA	Streptococcus mutans UA159	52.09	95.399	0.497
comYA	Streptococcus mutans UA140	52.09	95.399	0.497