Detailed information NTG0854833

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: LLWG2_RS11510
• Genome accession: NZ_CP129660
• Coordinates: 2220917..2221342 (-)
• Length: 141 a.a.
• NCBI ID: WP_014735174.1
• Uniprot ID: -
• Organism: Lactococcus cremoris strain Wg2
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
ICE	2216054..2224284	2220917..2221342	within	0

Gene organization within MGE regions

Location: 2216054..2224284

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLWG2_RS11485 (LLWG2_11540)	-	2217520..2218329 (-)	810	WP_011677177.1	metal ABC transporter permease	-
LLWG2_RS11490 (LLWG2_11545)	-	2218322..2219059 (-)	738	WP_011677178.1	metal ABC transporter ATP-binding protein	-
LLWG2_RS11495 (LLWG2_11550)	-	2219238..2220080 (-)	843	WP_021164979.1	metal ABC transporter solute-binding protein, Zn/Mn family	-
LLWG2_RS11500 (LLWG2_11555)	-	2220077..2220514 (-)	438	WP_011677180.1	zinc-dependent MarR family transcriptional regulator	-
LLWG2_RS11505 (LLWG2_11560)	comGG	2220594..2220821 (-)	228	WP_228764408.1	competence protein ComGG	Machinery gene
LLWG2_RS11510 (LLWG2_11565)	comGF	2220917..2221342 (-)	426	WP_014735174.1	competence type IV pilus minor pilin ComGF	Machinery gene
LLWG2_RS11515 (LLWG2_11570)	comGE	2221326..2221622 (-)	297	WP_021164977.1	competence type IV pilus minor pilin ComGE	Machinery gene
LLWG2_RS11520 (LLWG2_11575)	comGD	2221594..2221782 (-)	189	WP_014573336.1	hypothetical protein	Machinery gene
LLWG2_RS11525 (LLWG2_11580)	comGC	2221984..2222334 (-)	351	WP_050574187.1	competence type IV pilus major pilin ComGC	Machinery gene
LLWG2_RS11530 (LLWG2_11585)	comGB	2222379..2223404 (-)	1026	WP_050574185.1	competence type IV pilus assembly protein ComGB	Machinery gene
LLWG2_RS11535 (LLWG2_11590)	comGA	2223304..2224284 (-)	981	WP_021164974.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 141 a.a.
• Molecular weight: 16210.78 Da
• Isoelectric Point: 8.4549

>NTDB_id=854833 LLWG2_RS11510 WP_014735174.1 2220917..2221342(-) (comGF) [Lactococcus cremoris strain Wg2]
MKLKIRAFTLLECLVALLAISGSVLVISGLTRMIEEQMKISQNDSRKDWQIFCEQMRSELSGAKLDNVNQNFLYVTKDKK
LRFGLVGDDFRKSDDKGQGYQPMLYDLKGAKIQAEENLIKITIDFDNGGERVFIYRFTDTK

Nucleotide

• Length: 426 bp

>NTDB_id=854833 LLWG2_RS11510 WP_014735174.1 2220917..2221342(-) (comGF) [Lactococcus cremoris strain Wg2]
TTGAAGCTCAAAATTAGGGCATTTACTTTACTTGAATGTCTCGTAGCACTTTTGGCAATTTCTGGTTCAGTGCTAGTCAT
CTCAGGGCTGACTAGAATGATTGAAGAACAGATGAAAATAAGCCAGAATGATAGTAGGAAAGATTGGCAAATATTTTGTG
AACAAATGCGTTCAGAACTCTCCGGAGCAAAATTAGACAATGTGAATCAAAATTTTTTGTATGTGACCAAAGATAAGAAG
TTACGGTTCGGATTAGTAGGGGATGATTTTCGTAAAAGTGATGATAAAGGGCAAGGATACCAACCGATGCTTTATGATTT
AAAAGGAGCAAAAATTCAGGCAGAAGAAAATTTGATAAAAATAACAATTGATTTTGATAATGGAGGTGAGCGAGTATTTA
TTTATCGATTTACTGATACAAAGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (38-130)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	100	100	1
comYF	Streptococcus mutans UA140	43.972	100	0.44
comYF	Streptococcus mutans UA159	43.262	100	0.433
comGF/cglF	Streptococcus mitis NCTC 12261	39.286	99.291	0.39
comGF/cglF	Streptococcus mitis SK321	39.286	99.291	0.39
comGF/cglF	Streptococcus pneumoniae Rx1	37.857	99.291	0.376
comGF/cglF	Streptococcus pneumoniae D39	37.857	99.291	0.376
comGF/cglF	Streptococcus pneumoniae R6	37.857	99.291	0.376
comGF/cglF	Streptococcus pneumoniae TIGR4	37.857	99.291	0.376