NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	LLWG2_RS09045	Genome accession	NZ_CP129660
Coordinates	1774905..1775351 (-)	Length	148 a.a.
NCBI ID	WP_228763864.1	Uniprot ID	-
Organism	Lactococcus cremoris strain Wg2
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1769905..1780351

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLWG2_RS09015 (LLWG2_09075)	-	1770249..1770464 (-)	216	WP_014572212.1	F0F1 ATP synthase subunit C	-
LLWG2_RS09020 (LLWG2_09080)	-	1770647..1771423 (-)	777	WP_021165645.1	alpha/beta hydrolase family protein	-
LLWG2_RS09025 (LLWG2_09085)	-	1771707..1772012 (-)	306	Protein_1757	ComEC/Rec2 family competence protein	-
LLWG2_RS09030 (LLWG2_09090)	-	1772033..1772923 (-)	891	WP_064305213.1	IS982 family transposase	-
LLWG2_RS09035 (LLWG2_09095)	comEC	1773000..1773509 (-)	510	WP_021165649.1	MBL fold metallo-hydrolase	Machinery gene
LLWG2_RS09040 (LLWG2_09100)	comEC	1773506..1774777 (-)	1272	WP_324187043.1	ComEC/Rec2 family competence protein	Machinery gene
LLWG2_RS09045 (LLWG2_09105)	comEA	1774905..1775351 (-)	447	WP_228763864.1	helix-hairpin-helix domain-containing protein	Machinery gene
LLWG2_RS09050 (LLWG2_09110)	-	1775617..1776978 (-)	1362	WP_014572203.1	ABC transporter permease	-
LLWG2_RS09055 (LLWG2_09115)	-	1776975..1777907 (-)	933	WP_014572202.1	ABC transporter ATP-binding protein	-
LLWG2_RS09060 (LLWG2_09120)	-	1778013..1778411 (-)	399	WP_011676775.1	hypothetical protein	-
LLWG2_RS09065 (LLWG2_09125)	-	1778528..1779088 (-)	561	WP_021165651.1	GNAT family N-acetyltransferase	-

Sequence

Protein

Download Length: 148 a.a. Molecular weight: 16038.01 Da Isoelectric Point: 4.6909

                                >NTDB_id=854822 LLWG2_RS09045 WP_228763864.1 1774905..1775351(-) (comEA) [Lactococcus cremoris strain Wg2]

MVDLKGAVAKPNVYQISSDERLVDLIRQAGGFTDQADQKSINLSAKLKDEEVIYVPKLGESSSSESTDSPISSSVSNQVS

TTSGPKININKADLTELQKLTGIGQKKAQDIIDFRMKNGDFKSIEDLGKVSGFGDKTLEKLKDEISID

Nucleotide

Download Length: 447 bp

                                >NTDB_id=854822 LLWG2_RS09045 WP_228763864.1 1774905..1775351(-) (comEA) [Lactococcus cremoris strain Wg2]

ATGGTTGATTTGAAAGGAGCGGTTGCAAAACCTAATGTATACCAAATTTCGTCAGATGAGCGCCTTGTTGATTTAATTAG

GCAAGCGGGAGGATTTACTGACCAAGCAGACCAAAAATCAATCAATCTGTCAGCGAAACTTAAAGATGAAGAAGTGATTT

ATGTACCAAAACTTGGGGAAAGTTCAAGTTCAGAAAGTACTGACAGTCCTATTAGCAGCTCTGTCAGTAATCAAGTTTCA

ACGACAAGTGGTCCAAAAATAAATATCAATAAAGCAGACCTGACAGAATTACAAAAACTGACTGGAATTGGTCAAAAAAA

AGCGCAAGATATTATTGATTTTCGCATGAAAAATGGTGACTTTAAATCAATAGAGGATTTGGGTAAAGTATCTGGCTTTG

GGGATAAAACATTAGAAAAATTGAAAGATGAGATTTCTATTGATTAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(1-56)

Helix-hairpin-helix motif (HHH_3)

(84-145)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Lactococcus lactis subsp. cremoris KW2	96.622	100	0.966
comEA/celA/cilE	Streptococcus mitis SK321	51.02	99.324	0.507
comEA	Latilactobacillus sakei subsp. sakei 23K	44.512	100	0.493
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	48.98	99.324	0.486
comEA/celA/cilE	Streptococcus pneumoniae Rx1	48.299	99.324	0.48
comEA/celA/cilE	Streptococcus pneumoniae D39	48.299	99.324	0.48
comEA/celA/cilE	Streptococcus pneumoniae R6	48.299	99.324	0.48
comEA/celA/cilE	Streptococcus mitis NCTC 12261	47.619	99.324	0.473
comEA	Streptococcus thermophilus LMD-9	43.421	100	0.446
comEA	Staphylococcus aureus N315	41.781	98.649	0.412
comEA	Staphylococcus aureus MW2	41.781	98.649	0.412
comEA	Bacillus subtilis subsp. subtilis str. 168	40.816	99.324	0.405

Detailed information NTG0854822