Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   QY866_RS04720 Genome accession   NZ_CP129530
Coordinates   954821..955711 (+) Length   296 a.a.
NCBI ID   WP_100970233.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain A1262     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 949821..960711
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QY866_RS04690 (QY866_04685) - 950255..951325 (+) 1071 WP_142501005.1 hypothetical protein -
  QY866_RS04695 (QY866_04690) - 951325..952725 (+) 1401 WP_099947401.1 hypothetical protein -
  QY866_RS04705 (QY866_04700) - 952912..953286 (-) 375 WP_011375004.1 hypothetical protein -
  QY866_RS04710 (QY866_04705) - 953383..953883 (+) 501 WP_076632058.1 VanZ family protein -
  QY866_RS04715 (QY866_04710) - 953974..954705 (+) 732 WP_341844041.1 YebC/PmpR family DNA-binding transcriptional regulator -
  QY866_RS04720 (QY866_04715) comGA 954821..955711 (+) 891 WP_100970233.1 competence type IV pilus ATPase ComGA Machinery gene
  QY866_RS04725 (QY866_04720) comGB 955704..956711 (+) 1008 WP_076632061.1 type II secretion system F family protein Machinery gene
  QY866_RS04730 (QY866_04725) comGC 956708..957007 (+) 300 WP_076632062.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  QY866_RS04735 (QY866_04730) comGD 956979..957431 (+) 453 WP_076632063.1 competence type IV pilus minor pilin ComGD Machinery gene
  QY866_RS04740 (QY866_04735) comGE 957418..957723 (+) 306 WP_025016385.1 hypothetical protein Machinery gene
  QY866_RS04745 (QY866_04740) comGF 957698..958510 (+) 813 WP_337457506.1 competence type IV pilus minor pilin ComGF Machinery gene
  QY866_RS04750 (QY866_04745) - 958634..959644 (+) 1011 WP_011374994.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 32936.80 Da        Isoelectric Point: 6.4932

>NTDB_id=854095 QY866_RS04720 WP_100970233.1 954821..955711(+) (comGA) [Latilactobacillus sakei strain A1262]
MSTEEKLTTLLKHCAKQRVQDLYFTPTIGGWRLTERRQSDLVTNQELDKPTGTRYLNRLKYMAGMDISETRRAQTGRSEL
QLADQTIYLRLATVGDFLNRESLVIRFIYPIGAIYHCDDQQILAQLTQMSRQAGLILFAGPTGSGKTTSLYHLAQQSMSA
KMVVAIEDPIEIVAPEFLQLQVNDNAGLSYAELLKISLRLRPDTLIIGEIRDLETAQYAISAALSGHLVLSTIHARSTQG
VVARLLDLGISELQLRACLTGIAYQSLVTKDDVVQAHYELLTGPDYFSKKEGGCDV

Nucleotide


Download         Length: 891 bp        

>NTDB_id=854095 QY866_RS04720 WP_100970233.1 954821..955711(+) (comGA) [Latilactobacillus sakei strain A1262]
ATGTCAACTGAAGAAAAGCTAACAACTTTATTAAAGCATTGTGCTAAACAACGTGTTCAAGATCTTTATTTTACACCGAC
AATAGGTGGCTGGCGCTTGACGGAACGTCGTCAGAGCGATTTGGTCACCAATCAAGAATTGGATAAACCGACTGGAACGC
GTTATTTAAATCGACTGAAGTATATGGCGGGGATGGATATCAGCGAGACTCGGCGTGCGCAAACCGGCCGCTCGGAATTA
CAGCTAGCCGATCAAACAATTTATTTACGCCTAGCGACGGTCGGGGATTTTTTAAACCGCGAAAGCTTGGTCATTCGGTT
TATCTATCCAATTGGTGCCATCTATCATTGTGATGATCAACAGATCTTGGCGCAATTAACGCAGATGAGTCGACAGGCGG
GCTTAATTCTTTTCGCTGGGCCGACTGGTTCTGGTAAAACGACTAGTCTCTATCATCTGGCCCAACAATCAATGAGTGCC
AAGATGGTGGTTGCTATTGAAGATCCGATTGAAATTGTTGCACCTGAGTTTTTGCAACTGCAGGTTAATGATAATGCGGG
ATTGAGCTACGCCGAGCTATTAAAAATTAGTTTACGACTGCGACCGGATACGCTGATTATCGGTGAAATTCGCGATTTAG
AGACAGCTCAATATGCAATTTCTGCGGCCTTAAGTGGCCACCTGGTATTGAGTACTATTCACGCTCGGAGTACACAGGGC
GTGGTCGCGCGCTTATTAGATCTTGGTATTTCTGAGCTGCAATTGCGGGCCTGTCTAACGGGAATTGCCTACCAATCGTT
GGTGACAAAAGACGACGTTGTGCAAGCCCATTATGAATTATTAACTGGTCCAGATTATTTCTCAAAAAAAGAAGGTGGTT
GCGATGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Latilactobacillus sakei subsp. sakei 23K

98.649

100

0.986
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

41.241

92.568

0.382
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

42.264

89.527

0.378
  comGA Lactococcus lactis subsp. cremoris KW2

37.458

100

0.378
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

41.887

89.527

0.375
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

41.887

89.527

0.375
  comGA/cglA/cilD Streptococcus pneumoniae D39

41.887

89.527

0.375
  comGA/cglA/cilD Streptococcus pneumoniae R6

41.887

89.527

0.375
  comYA Streptococcus gordonii str. Challis substr. CH1

40.221

91.554

0.368
  comYA Streptococcus mutans UA140

38.909

92.905

0.361
  comYA Streptococcus mutans UA159

38.909

92.905

0.361