Detailed information NTG0851415

Bioinformatically predicted

Overview

• Name: comGA
• Type: Machinery gene
• Locus tag: LLD17_RS12360
• Genome accession: NZ_CP129182
• Coordinates: 2254539..2255519 (-)
• Length: 326 a.a.
• NCBI ID: WP_162494683.1
• Uniprot ID: -
• Organism: Lactococcus cremoris strain D.1.7
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
ICE	2246041..2255519	2254539..2255519	within	0

Gene organization within MGE regions

Location: 2246041..2255519

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLD17_RS12310 (LLD17_12360)	-	2248755..2249564 (-)	810	WP_011677177.1	metal ABC transporter permease	-
LLD17_RS12315 (LLD17_12365)	-	2249557..2250294 (-)	738	WP_011677178.1	metal ABC transporter ATP-binding protein	-
LLD17_RS12320 (LLD17_12370)	-	2250473..2251315 (-)	843	WP_011677179.1	metal ABC transporter solute-binding protein, Zn/Mn family	-
LLD17_RS12325 (LLD17_12375)	-	2251312..2251749 (-)	438	WP_011677180.1	zinc-dependent MarR family transcriptional regulator	-
LLD17_RS12330 (LLD17_12380)	comGG	2251829..2252056 (-)	228	WP_228764408.1	competence protein ComGG	Machinery gene
LLD17_RS12335 (LLD17_12385)	comGF	2252152..2252577 (-)	426	WP_014735174.1	competence type IV pilus minor pilin ComGF	Machinery gene
LLD17_RS12340 (LLD17_12390)	comGE	2252561..2252797 (-)	237	WP_014573335.1	competence type IV pilus minor pilin ComGE	Machinery gene
LLD17_RS12345 (LLD17_12395)	comGD	2252829..2253017 (-)	189	WP_014573336.1	hypothetical protein	Machinery gene
LLD17_RS12350 (LLD17_12400)	comGC	2253219..2253596 (-)	378	Protein_2265	competence type IV pilus major pilin ComGC	-
LLD17_RS12355 (LLD17_12405)	comGB	2253614..2254639 (-)	1026	WP_050574185.1	competence type IV pilus assembly protein ComGB	Machinery gene
LLD17_RS12360 (LLD17_12410)	comGA	2254539..2255519 (-)	981	WP_162494683.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 37019.32 Da
• Isoelectric Point: 5.9464

>NTDB_id=851415 LLD17_RS12360 WP_162494683.1 2254539..2255519(-) (comGA) [Lactococcus cremoris strain D.1.7]
MVQKKAQELIQKAIEMEASDIYLIASGNLYKIYIRQQLGRTLIEELNQEIGLALLTHFKFLAGMNTGERRRVQLGACWYE
LEGSSAKRLRLSTVGDFEGNESLVIRLLHDQKQELEFWFDDKLQDFRCKRGLYLFSGPVGSGKTSLMFDLAHRHFSNAQV
ITIEEPVELIESDFIQLQVNDVIGNSYDELIKLSLRHRPDLLIVGEIRDQQTARAVLRASLTGYTVFSTIHAASVKGVVQ
RLLELGLSDWELKNGLEAVVYQRLIAGKGVLDIAKSKFDTWSPKKWNEKIENLYANGHLTAIEAEREKISINQASKIDTT
DGESFE

Nucleotide

• Length: 981 bp

>NTDB_id=851415 LLD17_RS12360 WP_162494683.1 2254539..2255519(-) (comGA) [Lactococcus cremoris strain D.1.7]
ATGGTACAGAAAAAAGCACAAGAACTCATTCAAAAGGCAATTGAGATGGAGGCTTCTGATATTTATTTAATTGCTTCAGG
AAATCTTTATAAGATATATATTCGTCAACAATTAGGGCGAACTTTAATAGAGGAACTTAACCAAGAGATTGGTTTAGCAT
TACTTACTCATTTTAAATTTCTTGCTGGCATGAATACTGGTGAACGCCGGCGTGTTCAGCTGGGTGCTTGTTGGTATGAA
CTAGAGGGAAGTTCGGCAAAACGTTTGCGCCTTTCAACAGTGGGAGATTTTGAAGGAAATGAATCATTGGTTATCCGTCT
TTTGCATGACCAAAAACAAGAACTTGAGTTTTGGTTTGACGATAAACTTCAGGATTTTCGATGTAAACGAGGACTTTATT
TATTTTCTGGGCCAGTAGGGTCTGGGAAAACTTCACTAATGTTTGACTTAGCCCACCGTCATTTTTCAAATGCACAGGTT
ATCACTATTGAGGAGCCTGTAGAATTAATTGAATCTGATTTTATTCAGTTACAAGTTAATGATGTAATTGGGAATAGTTA
TGATGAATTAATTAAACTTTCTTTAAGACACCGACCAGATTTACTAATTGTTGGAGAAATTCGAGATCAGCAGACAGCTC
GTGCTGTATTGCGTGCAAGTTTAACAGGCTATACAGTTTTTTCTACTATCCATGCGGCTTCCGTGAAGGGAGTGGTTCAA
CGTTTGTTGGAATTGGGTTTGAGTGATTGGGAATTGAAAAATGGATTAGAGGCAGTTGTTTATCAACGGTTAATAGCGGG
AAAAGGAGTATTAGATATTGCGAAAAGTAAATTTGACACTTGGTCGCCGAAAAAATGGAATGAAAAGATTGAGAACTTAT
ATGCAAACGGACATCTTACGGCTATTGAGGCCGAAAGGGAAAAAATTAGCATTAATCAAGCAAGCAAAATTGATACAACT
GATGGGGAATCTTTTGAATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (9-265)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGA	Lactococcus lactis subsp. cremoris KW2	99.08	100	0.991
comGA/cglA	Streptococcus sobrinus strain NIDR 6715-7	56.452	95.092	0.537
comYA	Streptococcus gordonii str. Challis substr. CH1	54.43	96.933	0.528
comGA/cglA/cilD	Streptococcus pneumoniae Rx1	54.487	95.706	0.521
comGA/cglA/cilD	Streptococcus pneumoniae D39	54.487	95.706	0.521
comGA/cglA/cilD	Streptococcus pneumoniae R6	54.487	95.706	0.521
comGA/cglA/cilD	Streptococcus pneumoniae TIGR4	54.487	95.706	0.521
comGA/cglA/cilD	Streptococcus mitis NCTC 12261	54.487	95.706	0.521
comYA	Streptococcus mutans UA159	52.733	95.399	0.503
comYA	Streptococcus mutans UA140	52.733	95.399	0.503