Detailed information NTG0851411

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: LLD17_RS12335
• Genome accession: NZ_CP129182
• Coordinates: 2252152..2252577 (-)
• Length: 141 a.a.
• NCBI ID: WP_014735174.1
• Uniprot ID: -
• Organism: Lactococcus cremoris strain D.1.7
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
ICE	2246041..2255519	2252152..2252577	within	0

Gene organization within MGE regions

Location: 2246041..2255519

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLD17_RS12310 (LLD17_12360)	-	2248755..2249564 (-)	810	WP_011677177.1	metal ABC transporter permease	-
LLD17_RS12315 (LLD17_12365)	-	2249557..2250294 (-)	738	WP_011677178.1	metal ABC transporter ATP-binding protein	-
LLD17_RS12320 (LLD17_12370)	-	2250473..2251315 (-)	843	WP_011677179.1	metal ABC transporter solute-binding protein, Zn/Mn family	-
LLD17_RS12325 (LLD17_12375)	-	2251312..2251749 (-)	438	WP_011677180.1	zinc-dependent MarR family transcriptional regulator	-
LLD17_RS12330 (LLD17_12380)	comGG	2251829..2252056 (-)	228	WP_228764408.1	competence protein ComGG	Machinery gene
LLD17_RS12335 (LLD17_12385)	comGF	2252152..2252577 (-)	426	WP_014735174.1	competence type IV pilus minor pilin ComGF	Machinery gene
LLD17_RS12340 (LLD17_12390)	comGE	2252561..2252797 (-)	237	WP_014573335.1	competence type IV pilus minor pilin ComGE	Machinery gene
LLD17_RS12345 (LLD17_12395)	comGD	2252829..2253017 (-)	189	WP_014573336.1	hypothetical protein	Machinery gene
LLD17_RS12350 (LLD17_12400)	comGC	2253219..2253596 (-)	378	Protein_2265	competence type IV pilus major pilin ComGC	-
LLD17_RS12355 (LLD17_12405)	comGB	2253614..2254639 (-)	1026	WP_050574185.1	competence type IV pilus assembly protein ComGB	Machinery gene
LLD17_RS12360 (LLD17_12410)	comGA	2254539..2255519 (-)	981	WP_162494683.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 141 a.a.
• Molecular weight: 16210.78 Da
• Isoelectric Point: 8.4549

>NTDB_id=851411 LLD17_RS12335 WP_014735174.1 2252152..2252577(-) (comGF) [Lactococcus cremoris strain D.1.7]
MKLKIRAFTLLECLVALLAISGSVLVISGLTRMIEEQMKISQNDSRKDWQIFCEQMRSELSGAKLDNVNQNFLYVTKDKK
LRFGLVGDDFRKSDDKGQGYQPMLYDLKGAKIQAEENLIKITIDFDNGGERVFIYRFTDTK

Nucleotide

• Length: 426 bp

>NTDB_id=851411 LLD17_RS12335 WP_014735174.1 2252152..2252577(-) (comGF) [Lactococcus cremoris strain D.1.7]
TTGAAGCTCAAAATTAGGGCATTTACTTTACTTGAATGTCTCGTAGCACTTTTGGCAATTTCTGGTTCAGTGCTAGTCAT
CTCAGGGCTGACTAGAATGATTGAAGAACAGATGAAAATAAGCCAGAATGATAGTAGGAAAGATTGGCAAATATTTTGTG
AACAAATGCGTTCAGAACTCTCCGGAGCAAAATTAGACAATGTGAATCAAAATTTTTTGTATGTGACCAAAGATAAGAAG
TTACGGTTCGGATTAGTAGGGGATGATTTTCGTAAAAGTGATGATAAAGGGCAAGGATACCAACCGATGCTTTATGATTT
AAAAGGAGCAAAAATTCAGGCAGAAGAAAATTTGATAAAAATAACAATTGATTTTGATAATGGAGGTGAGCGAGTATTTA
TTTATCGATTTACTGATACAAAGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (38-130)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	100	100	1
comYF	Streptococcus mutans UA140	43.972	100	0.44
comYF	Streptococcus mutans UA159	43.262	100	0.433
comGF/cglF	Streptococcus mitis NCTC 12261	39.286	99.291	0.39
comGF/cglF	Streptococcus mitis SK321	39.286	99.291	0.39
comGF/cglF	Streptococcus pneumoniae Rx1	37.857	99.291	0.376
comGF/cglF	Streptococcus pneumoniae D39	37.857	99.291	0.376
comGF/cglF	Streptococcus pneumoniae R6	37.857	99.291	0.376
comGF/cglF	Streptococcus pneumoniae TIGR4	37.857	99.291	0.376