Detailed information NTG0850835

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LLUC320_RS09165
• Genome accession: NZ_CP129095
• Coordinates: 1797184..1797630 (-)
• Length: 148 a.a.
• NCBI ID: WP_228763864.1
• Uniprot ID: -
• Organism: Lactococcus cremoris strain UC320
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1792184..1802630

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLUC320_RS09135 (LLUC320_09180)	-	1792528..1792743 (-)	216	WP_014572212.1	F0F1 ATP synthase subunit C	-
LLUC320_RS09140 (LLUC320_09185)	-	1792926..1793702 (-)	777	WP_014572211.1	alpha/beta hydrolase family protein	-
LLUC320_RS09145 (LLUC320_09190)	-	1793986..1794291 (-)	306	Protein_1782	ComEC/Rec2 family competence protein	-
LLUC320_RS09150 (LLUC320_09195)	-	1794312..1795202 (-)	891	WP_281634794.1	IS982-like element IS982B family transposase	-
LLUC320_RS09155 (LLUC320_09200)	comEC	1795279..1795788 (-)	510	WP_021165649.1	MBL fold metallo-hydrolase	Machinery gene
LLUC320_RS09160 (LLUC320_09205)	comEC	1795785..1797056 (-)	1272	WP_324187043.1	ComEC/Rec2 family competence protein	Machinery gene
LLUC320_RS09165 (LLUC320_09210)	comEA	1797184..1797630 (-)	447	WP_228763864.1	helix-hairpin-helix domain-containing protein	Machinery gene
LLUC320_RS09170 (LLUC320_09215)	-	1797896..1799257 (-)	1362	WP_081196831.1	ABC transporter permease	-
LLUC320_RS09175 (LLUC320_09220)	-	1799254..1800186 (-)	933	WP_014572202.1	ABC transporter ATP-binding protein	-
LLUC320_RS09180 (LLUC320_09225)	-	1800292..1800690 (-)	399	WP_011676775.1	hypothetical protein	-
LLUC320_RS09185 (LLUC320_09230)	-	1800782..1801366 (-)	585	WP_081196832.1	GNAT family N-acetyltransferase	-

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 16038.01 Da
• Isoelectric Point: 4.6909

>NTDB_id=850835 LLUC320_RS09165 WP_228763864.1 1797184..1797630(-) (comEA) [Lactococcus cremoris strain UC320]
MVDLKGAVAKPNVYQISSDERLVDLIRQAGGFTDQADQKSINLSAKLKDEEVIYVPKLGESSSSESTDSPISSSVSNQVS
TTSGPKININKADLTELQKLTGIGQKKAQDIIDFRMKNGDFKSIEDLGKVSGFGDKTLEKLKDEISID

Nucleotide

• Length: 447 bp

>NTDB_id=850835 LLUC320_RS09165 WP_228763864.1 1797184..1797630(-) (comEA) [Lactococcus cremoris strain UC320]
ATGGTTGATTTGAAAGGAGCGGTTGCAAAACCTAATGTATACCAAATTTCGTCAGATGAGCGCCTTGTTGATTTAATTAG
GCAAGCGGGAGGATTTACTGACCAAGCAGACCAAAAATCAATCAATCTGTCAGCGAAACTTAAAGATGAAGAAGTGATTT
ATGTACCAAAACTTGGGGAAAGTTCAAGTTCAGAAAGTACTGACAGTCCTATTAGCAGCTCTGTCAGTAATCAAGTTTCA
ACGACAAGTGGTCCAAAAATAAATATCAATAAAGCAGACCTGACAGAATTACAAAAACTGACTGGAATTGGTCAAAAAAA
AGCGCAAGATATTATTGATTTTCGCATGAAAAATGGTGACTTTAAATCAATAGAGGATTTGGGTAAAGTATCTGGCTTTG
GGGATAAAACATTAGAAAAATTGAAAGATGAGATTTCTATTGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (1-56)

• Helix-hairpin-helix motif (HHH_3) (84-145)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	96.622	100	0.966
comEA/celA/cilE	Streptococcus mitis SK321	51.02	99.324	0.507
comEA	Latilactobacillus sakei subsp. sakei 23K	44.512	100	0.493
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	48.98	99.324	0.486
comEA/celA/cilE	Streptococcus pneumoniae Rx1	48.299	99.324	0.48
comEA/celA/cilE	Streptococcus pneumoniae D39	48.299	99.324	0.48
comEA/celA/cilE	Streptococcus pneumoniae R6	48.299	99.324	0.48
comEA/celA/cilE	Streptococcus mitis NCTC 12261	47.619	99.324	0.473
comEA	Streptococcus thermophilus LMD-9	43.421	100	0.446
comEA	Staphylococcus aureus N315	41.781	98.649	0.412
comEA	Staphylococcus aureus MW2	41.781	98.649	0.412
comEA	Bacillus subtilis subsp. subtilis str. 168	40.816	99.324	0.405