Detailed information NTG0850789

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LLUC310_RS09270
• Genome accession: NZ_CP129094
• Coordinates: 1704665..1705111 (-)
• Length: 148 a.a.
• NCBI ID: WP_228763864.1
• Uniprot ID: -
• Organism: Lactococcus cremoris strain UC310
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1699665..1710111

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLUC310_RS09240 (LLUC310_09310)	-	1700009..1700224 (-)	216	WP_014572212.1	F0F1 ATP synthase subunit C	-
LLUC310_RS09245 (LLUC310_09315)	-	1700407..1701183 (-)	777	WP_014572211.1	alpha/beta hydrolase family protein	-
LLUC310_RS09250 (LLUC310_09320)	-	1701467..1701772 (-)	306	Protein_1656	ComEC/Rec2 family competence protein	-
LLUC310_RS09255 (LLUC310_09325)	-	1701793..1702683 (-)	891	WP_347450349.1	IS982-like element ISLll1 family transposase	-
LLUC310_RS09260 (LLUC310_09330)	comEC	1702760..1703269 (-)	510	WP_021165649.1	MBL fold metallo-hydrolase	Machinery gene
LLUC310_RS09265 (LLUC310_09335)	comEC	1703266..1704537 (-)	1272	WP_345894098.1	ComEC/Rec2 family competence protein	Machinery gene
LLUC310_RS09270 (LLUC310_09340)	comEA	1704665..1705111 (-)	447	WP_228763864.1	helix-hairpin-helix domain-containing protein	Machinery gene
LLUC310_RS09275 (LLUC310_09345)	-	1705377..1706738 (-)	1362	WP_014572203.1	ABC transporter permease	-
LLUC310_RS09280 (LLUC310_09350)	-	1706735..1707667 (-)	933	WP_014572202.1	ABC transporter ATP-binding protein	-
LLUC310_RS09285 (LLUC310_09355)	-	1707773..1708171 (-)	399	WP_011676775.1	hypothetical protein	-
LLUC310_RS09290 (LLUC310_09360)	-	1708263..1708847 (-)	585	WP_134047437.1	GNAT family N-acetyltransferase	-

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 16038.01 Da
• Isoelectric Point: 4.6909

>NTDB_id=850789 LLUC310_RS09270 WP_228763864.1 1704665..1705111(-) (comEA) [Lactococcus cremoris strain UC310]
MVDLKGAVAKPNVYQISSDERLVDLIRQAGGFTDQADQKSINLSAKLKDEEVIYVPKLGESSSSESTDSPISSSVSNQVS
TTSGPKININKADLTELQKLTGIGQKKAQDIIDFRMKNGDFKSIEDLGKVSGFGDKTLEKLKDEISID

Nucleotide

• Length: 447 bp

>NTDB_id=850789 LLUC310_RS09270 WP_228763864.1 1704665..1705111(-) (comEA) [Lactococcus cremoris strain UC310]
ATGGTTGATTTGAAAGGAGCGGTTGCAAAACCTAATGTATACCAAATTTCGTCAGATGAGCGCCTTGTTGATTTAATTAG
GCAAGCGGGAGGATTTACTGACCAAGCAGACCAAAAATCAATCAATCTGTCAGCGAAACTTAAAGATGAAGAAGTGATTT
ATGTACCAAAACTTGGGGAAAGTTCAAGTTCAGAAAGTACTGACAGTCCTATTAGCAGCTCTGTCAGTAATCAAGTTTCA
ACGACAAGTGGTCCAAAAATAAATATCAATAAAGCAGACCTGACAGAATTACAAAAACTGACTGGAATTGGTCAAAAAAA
AGCGCAAGATATTATTGATTTTCGCATGAAAAATGGTGACTTTAAATCAATAGAGGATTTGGGTAAAGTATCTGGCTTTG
GGGATAAAACATTAGAAAAATTGAAAGATGAGATTTCTATTGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (1-56)

• Helix-hairpin-helix motif (HHH_3) (84-145)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	96.622	100	0.966
comEA/celA/cilE	Streptococcus mitis SK321	51.02	99.324	0.507
comEA	Latilactobacillus sakei subsp. sakei 23K	44.512	100	0.493
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	48.98	99.324	0.486
comEA/celA/cilE	Streptococcus pneumoniae Rx1	48.299	99.324	0.48
comEA/celA/cilE	Streptococcus pneumoniae D39	48.299	99.324	0.48
comEA/celA/cilE	Streptococcus pneumoniae R6	48.299	99.324	0.48
comEA/celA/cilE	Streptococcus mitis NCTC 12261	47.619	99.324	0.473
comEA	Streptococcus thermophilus LMD-9	43.421	100	0.446
comEA	Staphylococcus aureus N315	41.781	98.649	0.412
comEA	Staphylococcus aureus MW2	41.781	98.649	0.412
comEA	Bacillus subtilis subsp. subtilis str. 168	40.816	99.324	0.405