Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   QLQ55_RS09130 Genome accession   NZ_CP124886
Coordinates   1814876..1815151 (-) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain EfsPF13     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1771798..1814852 1814876..1815151 flank 24


Gene organization within MGE regions


Location: 1771798..1815151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QLQ55_RS08845 (QLQ55_08845) - 1771798..1772805 (-) 1008 WP_002357063.1 class I SAM-dependent methyltransferase -
  QLQ55_RS08850 (QLQ55_08850) comGG 1772934..1773287 (-) 354 WP_002357061.1 competence type IV pilus minor pilin ComGG -
  QLQ55_RS08855 (QLQ55_08855) comGF 1773287..1773721 (-) 435 WP_002357060.1 competence type IV pilus minor pilin ComGF -
  QLQ55_RS08860 (QLQ55_08860) - 1773711..1774082 (-) 372 WP_170310356.1 type II secretion system protein -
  QLQ55_RS08865 (QLQ55_08865) hemH 1774837..1775778 (-) 942 WP_002357056.1 ferrochelatase -
  QLQ55_RS08875 (QLQ55_08875) - 1776515..1776766 (-) 252 WP_002357054.1 hypothetical protein -
  QLQ55_RS08880 (QLQ55_08880) - 1776838..1777038 (-) 201 WP_002357053.1 cold-shock protein -
  QLQ55_RS08885 (QLQ55_08885) - 1777875..1779113 (-) 1239 WP_002357052.1 LysM peptidoglycan-binding domain-containing protein -
  QLQ55_RS08890 (QLQ55_08890) - 1779126..1779506 (-) 381 WP_010706839.1 phage holin family protein -
  QLQ55_RS08895 (QLQ55_08895) - 1779517..1779639 (-) 123 WP_002357050.1 XkdX family protein -
  QLQ55_RS08900 (QLQ55_08900) - 1779639..1780013 (-) 375 WP_002357049.1 hypothetical protein -
  QLQ55_RS08905 (QLQ55_08905) - 1780030..1780542 (-) 513 WP_002357048.1 hypothetical protein -
  QLQ55_RS08910 (QLQ55_08910) - 1780542..1780829 (-) 288 WP_002357046.1 collagen-like protein -
  QLQ55_RS08915 (QLQ55_08915) - 1780826..1781422 (-) 597 WP_002357045.1 hypothetical protein -
  QLQ55_RS08920 (QLQ55_08920) - 1781415..1782296 (-) 882 WP_002357044.1 phage baseplate upper protein -
  QLQ55_RS08925 (QLQ55_08925) - 1782315..1785122 (-) 2808 WP_002384367.1 phage tail spike protein -
  QLQ55_RS08930 (QLQ55_08930) - 1785104..1785838 (-) 735 WP_002357042.1 hypothetical protein -
  QLQ55_RS08935 (QLQ55_08935) - 1785828..1788725 (-) 2898 WP_002385823.1 tape measure protein -
  QLQ55_RS08940 (QLQ55_08940) - 1788973..1789323 (-) 351 WP_002357039.1 hypothetical protein -
  QLQ55_RS08945 (QLQ55_08945) - 1789376..1790224 (-) 849 WP_002385822.1 major tail protein -
  QLQ55_RS08950 (QLQ55_08950) - 1790225..1790599 (-) 375 WP_002357037.1 DUF6838 family protein -
  QLQ55_RS08955 (QLQ55_08955) - 1790602..1791000 (-) 399 WP_002357036.1 HK97 gp10 family phage protein -
  QLQ55_RS08960 (QLQ55_08960) - 1790993..1791361 (-) 369 WP_002357034.1 hypothetical protein -
  QLQ55_RS08965 (QLQ55_08965) - 1791358..1791627 (-) 270 WP_264516855.1 hypothetical protein -
  QLQ55_RS08970 (QLQ55_08970) - 1791715..1791897 (-) 183 WP_002357032.1 hypothetical protein -
  QLQ55_RS08975 (QLQ55_08975) - 1791926..1792813 (-) 888 WP_002357030.1 DUF5309 domain-containing protein -
  QLQ55_RS08980 (QLQ55_08980) - 1792827..1793450 (-) 624 WP_002389133.1 DUF4355 domain-containing protein -
  QLQ55_RS08985 (QLQ55_08985) - 1793668..1793988 (-) 321 WP_002389265.1 hypothetical protein -
  QLQ55_RS08990 (QLQ55_08990) - 1794053..1794274 (-) 222 WP_002357027.1 hypothetical protein -
  QLQ55_RS08995 (QLQ55_08995) - 1794271..1796028 (-) 1758 WP_002384360.1 head protein -
  QLQ55_RS09000 (QLQ55_09000) - 1796003..1797490 (-) 1488 WP_002384358.1 phage portal protein -
  QLQ55_RS09005 (QLQ55_09005) - 1797502..1798791 (-) 1290 WP_002357024.1 PBSX family phage terminase large subunit -
  QLQ55_RS09010 (QLQ55_09010) terS 1798763..1799566 (-) 804 WP_002357023.1 phage terminase small subunit -
  QLQ55_RS09015 (QLQ55_09015) - 1799625..1799984 (-) 360 WP_002357022.1 hypothetical protein -
  QLQ55_RS09020 (QLQ55_09020) - 1800894..1801565 (+) 672 WP_002398611.1 hypothetical protein -
  QLQ55_RS09025 (QLQ55_09025) - 1801558..1802367 (-) 810 WP_002388196.1 hypothetical protein -
  QLQ55_RS09030 (QLQ55_09030) - 1803000..1803416 (-) 417 WP_002388198.1 ArpU family phage packaging/lysis transcriptional regulator -
  QLQ55_RS09035 (QLQ55_09035) - 1804324..1804752 (-) 429 WP_002364342.1 RusA family crossover junction endodeoxyribonuclease -
  QLQ55_RS09040 (QLQ55_09040) - 1804749..1805606 (-) 858 WP_002364343.1 helix-turn-helix domain-containing protein -
  QLQ55_RS09045 (QLQ55_09045) - 1805606..1805806 (-) 201 WP_002357010.1 hypothetical protein -
  QLQ55_RS09050 (QLQ55_09050) - 1805811..1806452 (-) 642 WP_002364344.1 putative HNHc nuclease -
  QLQ55_RS09055 (QLQ55_09055) - 1806457..1807191 (-) 735 WP_002385820.1 ERF family protein -
  QLQ55_RS09060 (QLQ55_09060) - 1807184..1807501 (-) 318 WP_002357007.1 hypothetical protein -
  QLQ55_RS09065 (QLQ55_09065) - 1807721..1808275 (+) 555 WP_002357006.1 hypothetical protein -
  QLQ55_RS09070 (QLQ55_09070) - 1808560..1808898 (-) 339 WP_002357003.1 hypothetical protein -
  QLQ55_RS09075 (QLQ55_09075) - 1808935..1809144 (-) 210 WP_002357002.1 hypothetical protein -
  QLQ55_RS09080 (QLQ55_09080) - 1809199..1809387 (+) 189 WP_002357001.1 YegP family protein -
  QLQ55_RS09085 (QLQ55_09085) - 1809413..1810135 (-) 723 WP_002357000.1 phage regulatory protein -
  QLQ55_RS09090 (QLQ55_09090) - 1810158..1810475 (-) 318 WP_002356999.1 hypothetical protein -
  QLQ55_RS09095 (QLQ55_09095) - 1810481..1810672 (-) 192 WP_002356998.1 hypothetical protein -
  QLQ55_RS09100 (QLQ55_09100) - 1810963..1811307 (+) 345 WP_002385819.1 helix-turn-helix transcriptional regulator -
  QLQ55_RS09105 (QLQ55_09105) - 1811312..1811959 (+) 648 WP_002356996.1 ImmA/IrrE family metallo-endopeptidase -
  QLQ55_RS09110 (QLQ55_09110) - 1812077..1812841 (+) 765 WP_010711480.1 LysM domain-containing protein -
  QLQ55_RS09115 (QLQ55_09115) - 1812856..1813185 (+) 330 WP_002356994.1 hypothetical protein -
  QLQ55_RS09120 (QLQ55_09120) - 1813260..1814408 (+) 1149 WP_002389015.1 site-specific integrase -
  QLQ55_RS09125 (QLQ55_09125) comGD 1814436..1814879 (-) 444 WP_002356992.1 competence type IV pilus minor pilin ComGD -
  QLQ55_RS09130 (QLQ55_09130) comGC/cglC 1814876..1815151 (-) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=828719 QLQ55_RS09130 WP_002356991.1 1814876..1815151(-) (comGC/cglC) [Enterococcus faecalis strain EfsPF13]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=828719 QLQ55_RS09130 WP_002356991.1 1814876..1815151(-) (comGC/cglC) [Enterococcus faecalis strain EfsPF13]
ATGAAAAAGAAACAAAAATACGCAGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTCCCTAACTTAGCGAAACATAAAGAAACAGTTGATAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCCTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396