Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QJ050_RS03140 Genome accession   NZ_CP124840
Coordinates   673516..674655 (-) Length   379 a.a.
NCBI ID   WP_282790038.1    Uniprot ID   -
Organism   Aeromonas salmonicida strain 29     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 668516..679655
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QJ050_RS03115 (QJ050_03115) - 668516..668743 (+) 228 WP_005314583.1 FeoA family protein -
  QJ050_RS03120 (QJ050_03120) feoB 668740..671010 (+) 2271 WP_282790036.1 Fe(2+) transporter permease subunit FeoB -
  QJ050_RS03125 (QJ050_03125) - 671007..671249 (+) 243 WP_021140137.1 FeoC-like transcriptional regulator -
  QJ050_RS03130 (QJ050_03130) - 671346..671825 (-) 480 WP_282790037.1 WbuC family cupin fold metalloprotein -
  QJ050_RS03135 (QJ050_03135) glgC 671972..673243 (-) 1272 WP_017412109.1 glucose-1-phosphate adenylyltransferase -
  QJ050_RS03140 (QJ050_03140) pilU 673516..674655 (-) 1140 WP_282790038.1 type IVa pilus ATPase TapW Machinery gene
  QJ050_RS03145 (QJ050_03145) - 674715..675071 (-) 357 WP_005314602.1 DMT family protein -
  QJ050_RS03150 (QJ050_03150) - 675183..675914 (+) 732 WP_005314604.1 transporter substrate-binding domain-containing protein -
  QJ050_RS03155 (QJ050_03155) btuC 676018..677025 (+) 1008 WP_282790039.1 vitamin B12 ABC transporter permease BtuC -
  QJ050_RS03160 (QJ050_03160) - 677085..677894 (+) 810 WP_282790727.1 ATP-binding cassette domain-containing protein -
  QJ050_RS03165 (QJ050_03165) - 678011..679072 (-) 1062 WP_282790040.1 3-deoxy-7-phosphoheptulonate synthase -
  QJ050_RS03170 (QJ050_03170) - 679227..679607 (+) 381 WP_058394146.1 diacylglycerol kinase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42239.23 Da        Isoelectric Point: 6.4288

>NTDB_id=828276 QJ050_RS03140 WP_282790038.1 673516..674655(-) (pilU) [Aeromonas salmonicida strain 29]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETSLEPGEVAKIAADIMDGEQKQQFERELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPAVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGSLKEVMEKSRALGMVTFDSAL
FDLVVEGVIEEEEAVKNADSANNLRLKIKLWKEKGQIANSADATGWSLEPTKDESNDPF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=828276 QJ050_RS03140 WP_282790038.1 673516..674655(-) (pilU) [Aeromonas salmonicida strain 29]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGACGGCTCAGACCTCTATCTGTCGACCGGCGCCCCTCCCTG
TGCCAAGTTCAACGGTGGGTTGCGTCCCCTGTCCGAGACGTCGCTCGAACCGGGGGAAGTGGCCAAGATAGCCGCCGACA
TCATGGATGGCGAGCAGAAGCAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGGCGT
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTCTCCCTGGTGGCCCGCAACATCAAGACAGAGATCCCCCGCTTTGA
GGATCTCAAACTGCCGGCCGTGCTGCTCGACACCATCATGGAGAAACGCGGCCTGGTGCTGTTTGTGGGCGGAACAGGCT
CGGGCAAATCCACGTCGCTGGCGGCCCTCATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTCCATCGTCACCGCAAGAGCATCATCAACCAGCGGGAGGTGGGGGTAGATACCCGCAGCTTCCACGC
CGCGCTCAAGAACACCCTTCGCCAGGCACCGGATGTGATCCTCATCGGTGAAATTCGCGATCGCGAGACCATGGAGCATG
CCCTTGCCTTCTCCGAGACCGGGCATCTGGCCATCTCTACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCACAGTTGCTCAACGATCTTGGCAACAACCTCAAGGCCTTTGTCTCCCAGCG
ACTGGTGAAAACCACAGACGGTGGCCGCCGGGCGGCGGTGGAGATCATGCTGGGCACCCACACCATCCGCGACATGATCA
AACGCGGCGAATTTGGCAGCCTGAAAGAGGTGATGGAGAAATCCAGAGCGCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTGGAAGGGGTGATAGAGGAGGAAGAAGCGGTGAAAAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAAGAGAAGGGCCAGATCGCCAACAGTGCGGATGCCACCGGCTGGAGTCTGGAGCCCACCAAGGACG
AAAGTAACGATCCCTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.158

100

0.533
  pilU Acinetobacter baylyi ADP1

52.958

93.668

0.496
  pilU Vibrio cholerae strain A1552

50

92.348

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.476

99.736

0.404
  pilT Pseudomonas aeruginosa PAK

40.698

90.765

0.369
  pilT Legionella pneumophila strain Lp02

41.916

88.127

0.369
  pilT Legionella pneumophila strain ERS1305867

41.916

88.127

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.407

90.765

0.367
  pilT Acinetobacter baumannii strain A118

41.018

88.127

0.361
  pilT Acinetobacter baumannii D1279779

41.018

88.127

0.361
  pilT Acinetobacter nosocomialis M2

41.018

88.127

0.361