Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PRtIB026_RS04855 Genome accession   NZ_AP023348
Coordinates   1060359..1061582 (+) Length   407 a.a.
NCBI ID   WP_267813897.1    Uniprot ID   -
Organism   Pseudomonas sp. RtIB026     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1055359..1066582
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PRtIB026_RS04840 (PRtIB026_52840) pilM 1057957..1058853 (+) 897 WP_203648498.1 type IV pilus biogenesis protein PilM -
  PRtIB026_RS04845 (PRtIB026_52830) - 1058850..1059389 (+) 540 WP_203648496.1 PilN domain-containing protein -
  PRtIB026_RS04850 (PRtIB026_52820) - 1059386..1060402 (+) 1017 WP_203648493.1 pilus assembly protein PilP -
  PRtIB026_RS04855 (PRtIB026_A09530) pilQ 1060359..1061582 (+) 1224 WP_267813897.1 type IV pilus secretin PilQ Machinery gene
  PRtIB026_RS04860 (PRtIB026_52790) aroK 1061587..1062105 (+) 519 WP_186712005.1 shikimate kinase AroK -
  PRtIB026_RS04865 (PRtIB026_52780) aroB 1062200..1063297 (+) 1098 WP_203648491.1 3-dehydroquinate synthase -
  PRtIB026_RS04870 (PRtIB026_52770) - 1063313..1064935 (+) 1623 WP_203648484.1 AAA family ATPase -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 44257.86 Da        Isoelectric Point: 4.9150

>NTDB_id=82511 PRtIB026_RS04855 WP_267813897.1 1060359..1061582(+) (pilQ) [Pseudomonas sp. RtIB026]
MLGLQMAQAETFQGEPMSLNFQDVELRSVLQVMADHAGINLVAGDSVQGRITLRLEDVPWGQALDLVLRSKGLGRRQEGN
VLLVAPLAELAGQAQDMRQEVLLEPLQRELIPVFHAKAGELAELLLASLADDGALAGRGSLSVDVRTNTLVATQPAARLA
ELRRLVTQLDVPVRQVMIEARIVEANVDYDKALGVRWGGPLYGAERRLGSNLFVDLGVERAGAGLGVGLLRSDILLDLEL
SAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETTRSGATSVSFREASLSLEVTPQITPDDKVIMAVRVTKDEPDF
INALNDVPPIRKNEVNAKVRVTDGQTIVIGGVYSTSQNKVVDKVPFFGDLPYVGRLFRRDALQEKKSELLVFLTPRIMSD
QAIAVSR

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=82511 PRtIB026_RS04855 WP_267813897.1 1060359..1061582(+) (pilQ) [Pseudomonas sp. RtIB026]
ATGCTGGGGCTGCAGATGGCGCAGGCCGAGACGTTTCAGGGTGAGCCGATGTCGCTCAACTTCCAGGATGTCGAACTGCG
TTCGGTGCTCCAGGTCATGGCCGACCACGCGGGCATCAACCTGGTGGCCGGCGATTCGGTGCAGGGGCGGATAACCCTGC
GTCTGGAGGATGTGCCCTGGGGCCAGGCGCTTGACCTGGTGTTGCGCAGCAAGGGGCTGGGCAGGCGCCAGGAGGGGAAT
GTGCTGCTGGTCGCTCCCCTGGCCGAGCTCGCCGGGCAAGCCCAGGACATGCGTCAGGAGGTATTGCTGGAGCCTCTGCA
GCGTGAGTTGATCCCTGTGTTCCATGCCAAGGCCGGCGAGCTGGCGGAGCTGTTGCTGGCGAGCCTGGCTGACGACGGTG
CCCTGGCCGGGCGTGGCAGCCTCAGTGTGGATGTACGCACCAATACACTGGTAGCCACCCAGCCTGCCGCCCGGCTCGCC
GAACTGCGCAGGCTGGTCACACAGCTGGATGTGCCGGTGCGCCAGGTCATGATCGAGGCACGGATCGTCGAGGCCAATGT
CGATTACGATAAGGCCTTGGGCGTGCGCTGGGGTGGACCGCTGTATGGCGCGGAGCGCCGGCTGGGCAGCAACCTGTTCG
TCGACCTCGGGGTCGAGCGGGCTGGTGCCGGGCTCGGGGTGGGGCTGCTGCGCAGCGATATCCTGCTCGACCTGGAGCTC
AGCGCCATGGAAAAAAGCGGCAATGGCGAGATCATCTCCCAGCCCAAGGTGGTCACGGCCGACAAGGAAACCGCGCGTAT
CCTCAAGGGCACCGAAGTGCCCTACCAGGAAACCACCCGCAGCGGCGCCACGTCGGTCTCCTTCCGCGAGGCGTCCCTGT
CGCTGGAGGTGACCCCGCAGATCACGCCGGACGACAAGGTGATCATGGCGGTGCGGGTGACCAAGGACGAGCCGGACTTC
ATCAATGCCCTCAACGATGTGCCGCCGATTCGCAAGAACGAGGTCAATGCCAAGGTGCGGGTAACCGATGGGCAGACCAT
TGTGATTGGTGGCGTGTACTCGACGTCGCAGAACAAAGTGGTCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTG
GACGGCTGTTTCGACGCGATGCATTACAAGAGAAAAAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGAC
CAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(110-175)

Bacterial type II and III secretion system protein (Secretin)

(242-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.257

100

0.624
  pilQ Acinetobacter baumannii D1279779

41.631

100

0.477
  pilQ Acinetobacter baumannii strain A118

41.631

100

0.477
  comQ Acinetobacter baylyi ADP1

42.14

100

0.474
  pilQ Legionella pneumophila strain ERS1305867

43.602

100

0.452
  pilQ Legionella pneumophila strain Lp02

43.128

100

0.447
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

40.618

100

0.42
  pilQ Vibrio cholerae strain A1552

40.618

100

0.42
  pilQ Vibrio campbellii strain DS40M4

39.86

100

0.42
  pilQ Neisseria gonorrhoeae strain FA1090

36.256

100

0.376
  pilQ Neisseria meningitidis MC58

35.238

100

0.364
  pilQ Neisseria meningitidis M1080

35.238

100

0.364

Multiple sequence alignment