Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   QCL65_RS21425 Genome accession   NZ_CP124218
Coordinates   4641755..4642234 (-) Length   159 a.a.
NCBI ID   WP_407312361.1    Uniprot ID   -
Organism   Pseudomonas sp. nanlin1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4636755..4647234
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QCL65_RS21385 (QCL65_21215) - 4636789..4637064 (+) 276 WP_407312353.1 hypothetical protein -
  QCL65_RS21390 (QCL65_21220) - 4637079..4637864 (-) 786 WP_407312355.1 EAL domain-containing protein -
  QCL65_RS21395 (QCL65_21225) - 4638064..4638291 (-) 228 WP_407316969.1 hypothetical protein -
  QCL65_RS21400 (QCL65_21230) - 4638566..4639759 (+) 1194 WP_407312357.1 O-antigen ligase family protein -
  QCL65_RS21405 (QCL65_21235) - 4639772..4640662 (-) 891 WP_407312359.1 glycosyltransferase family 2 protein -
  QCL65_RS21415 (QCL65_21245) - 4641053..4641121 (+) 69 Protein_4216 transcriptional regulator -
  QCL65_RS21420 (QCL65_21250) - 4641136..4641318 (+) 183 WP_407317026.1 hypothetical protein -
  QCL65_RS21425 (QCL65_21255) pilA2 4641755..4642234 (-) 480 WP_407312361.1 pilin Machinery gene
  QCL65_RS21430 (QCL65_21260) pilB 4642408..4644108 (+) 1701 WP_407312363.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QCL65_RS21435 (QCL65_21265) pilC 4644111..4645331 (+) 1221 WP_407312365.1 type II secretion system F family protein Machinery gene
  QCL65_RS21440 (QCL65_21270) pilD 4645332..4646201 (+) 870 WP_407312367.1 prepilin peptidase Machinery gene
  QCL65_RS21445 (QCL65_21275) coaE 4646201..4646824 (+) 624 WP_407312369.1 dephospho-CoA kinase -
  QCL65_RS21450 (QCL65_21280) yacG 4646821..4647024 (+) 204 WP_407312371.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16267.80 Da        Isoelectric Point: 9.0118

>NTDB_id=823951 QCL65_RS21425 WP_407312361.1 4641755..4642234(-) (pilA2) [Pseudomonas sp. nanlin1]
MQLPIQVKGHGAPIKGWVDMKGQKGFTLIELMIVVAIIGILAAVALPAYQDYTVRAQVSELFTVSEGAKVAVVEAYHNTG
ILPANNAAANNFAGAAGKYTASVNIGAGGQITATAGNAANNVIAAKTVTLSPLLDATGVITWTCAGSIDAKYRPASCRP

Nucleotide


Download         Length: 480 bp        

>NTDB_id=823951 QCL65_RS21425 WP_407312361.1 4641755..4642234(-) (pilA2) [Pseudomonas sp. nanlin1]
GTGCAATTACCCATTCAGGTCAAGGGGCATGGTGCTCCGATTAAAGGATGGGTAGATATGAAAGGGCAAAAGGGTTTTAC
GCTGATTGAATTGATGATCGTTGTGGCGATTATTGGGATTTTGGCTGCGGTGGCGTTGCCGGCTTATCAGGATTATACGG
TGCGGGCGCAGGTGAGTGAACTGTTCACCGTAAGTGAGGGGGCTAAGGTAGCGGTCGTCGAGGCTTACCATAATACCGGC
ATCCTACCTGCCAATAACGCGGCAGCTAATAACTTTGCAGGTGCTGCAGGCAAATACACCGCAAGTGTGAACATCGGTGC
TGGAGGTCAAATTACGGCAACTGCGGGTAACGCGGCGAACAACGTCATCGCTGCGAAGACTGTAACGTTATCACCACTCT
TAGACGCAACTGGCGTGATAACTTGGACTTGTGCGGGCTCTATCGATGCTAAATATCGCCCTGCTAGCTGCCGTCCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(20-46)

Pilin (bacterial filament) (Pilin)

(54-158)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

55.797

86.792

0.484
  pilA2 Legionella pneumophila strain ERS1305867

55.072

86.792

0.478
  pilE Neisseria gonorrhoeae MS11

44.586

98.742

0.44
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.297

100

0.434
  pilA/pilA1 Eikenella corrodens VA1

43.59

98.113

0.428
  pilE Neisseria gonorrhoeae strain FA1090

43.226

97.484

0.421
  comP Acinetobacter baylyi ADP1

43.226

97.484

0.421
  pilA Ralstonia pseudosolanacearum GMI1000

39.053

100

0.415