Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QCL65_RS21430 Genome accession   NZ_CP124218
Coordinates   4642408..4644108 (+) Length   566 a.a.
NCBI ID   WP_407312363.1    Uniprot ID   -
Organism   Pseudomonas sp. nanlin1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4637408..4649108
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QCL65_RS21395 (QCL65_21225) - 4638064..4638291 (-) 228 WP_407316969.1 hypothetical protein -
  QCL65_RS21400 (QCL65_21230) - 4638566..4639759 (+) 1194 WP_407312357.1 O-antigen ligase family protein -
  QCL65_RS21405 (QCL65_21235) - 4639772..4640662 (-) 891 WP_407312359.1 glycosyltransferase family 2 protein -
  QCL65_RS21415 (QCL65_21245) - 4641053..4641121 (+) 69 Protein_4216 transcriptional regulator -
  QCL65_RS21420 (QCL65_21250) - 4641136..4641318 (+) 183 WP_407317026.1 hypothetical protein -
  QCL65_RS21425 (QCL65_21255) pilA2 4641755..4642234 (-) 480 WP_407312361.1 pilin Machinery gene
  QCL65_RS21430 (QCL65_21260) pilB 4642408..4644108 (+) 1701 WP_407312363.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QCL65_RS21435 (QCL65_21265) pilC 4644111..4645331 (+) 1221 WP_407312365.1 type II secretion system F family protein Machinery gene
  QCL65_RS21440 (QCL65_21270) pilD 4645332..4646201 (+) 870 WP_407312367.1 prepilin peptidase Machinery gene
  QCL65_RS21445 (QCL65_21275) coaE 4646201..4646824 (+) 624 WP_407312369.1 dephospho-CoA kinase -
  QCL65_RS21450 (QCL65_21280) yacG 4646821..4647024 (+) 204 WP_407312371.1 DNA gyrase inhibitor YacG -
  QCL65_RS21455 (QCL65_21285) - 4647119..4647748 (+) 630 WP_407312373.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62725.68 Da        Isoelectric Point: 5.5626

>NTDB_id=823952 QCL65_RS21430 WP_407312363.1 4642408..4644108(+) (pilB) [Pseudomonas sp. nanlin1]
MNDIALTGLAKQLVLAELITDQSAQQAYQQAQRARMPLVHYLVQNKLISSRDIAEVAADQFGVSLLDLDTLDKEIQPRGL
VSEKLVRQHHALPLWRRGNKLFVGVSDPTNHQATTDIQFSTGLTTEAILVEDDKLMHAIDRFFDNAGTGLEELTDVDLDG
LDVETVDENASENVSRQDADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGILREMAKPPINLAGRIAARL
KVMASLDISERRKPQDGRIKMRISKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEPHQKELYLHALSQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINMEGINQVNVNNRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLQNMGVASFNIATSVNLIIAQRLARKLCGLCKKELELPREVQIEEGFPE
AQIGTFKIYQPVGCDQCNGGYKGRQGIYEVVKVTKELQRLIMESGNSIEIAAQMRKEGFNDLRTSGLVKVMQGVTSLAEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=823952 QCL65_RS21430 WP_407312363.1 4642408..4644108(+) (pilB) [Pseudomonas sp. nanlin1]
ATGAACGACATCGCCCTCACCGGTCTGGCCAAGCAATTGGTGCTGGCCGAGTTGATCACCGATCAAAGCGCCCAGCAGGC
CTATCAACAGGCGCAGCGGGCGCGTATGCCGCTGGTGCATTACCTGGTGCAGAACAAGCTGATCAGTAGCCGGGACATTG
CAGAGGTCGCGGCGGATCAGTTTGGCGTGTCGTTGCTAGACCTAGACACTCTGGATAAAGAGATTCAGCCCCGTGGCCTG
GTGAGTGAGAAGCTGGTGCGTCAGCACCATGCGCTGCCGTTGTGGCGGCGGGGCAATAAATTGTTTGTGGGGGTGTCGGA
CCCAACCAATCACCAGGCGACGACTGATATTCAGTTCAGTACCGGGCTGACCACCGAAGCGATTTTGGTGGAAGACGACA
AGTTGATGCACGCCATCGACCGCTTCTTCGACAATGCGGGCACGGGCCTGGAAGAGTTGACTGACGTTGACCTGGACGGG
CTGGACGTCGAGACGGTGGACGAGAACGCCAGCGAAAACGTGTCGCGGCAAGATGCCGACGATGCGCCGGTGGTGCGTTT
CGTCAATAAGATGCTGCTCGACGCGATTCGTGGCGGTTCTTCGGACTTGCACTTCGAGCCTTATGAAAAAACCTACCGGG
TGCGGTTTCGTACCGACGGTATCTTGCGCGAGATGGCCAAACCGCCGATCAACCTGGCCGGGCGGATTGCCGCGCGCTTG
AAGGTGATGGCCAGCCTGGATATTTCCGAACGGCGCAAGCCGCAGGATGGCCGGATCAAGATGCGCATTTCCAAGACCAA
GGCCATCGACTTCCGGGTCAATACCTTGCCCACCTTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACCCGTCCAGTG
CACAAATGGGCATCGACGCGTTGGGGTATGAGCCCCACCAGAAAGAGTTGTACTTGCATGCCCTTAGTCAGCCTCAGGGG
ATGATTCTGGTGACCGGGCCGACCGGGTCGGGTAAGACGGTATCTCTGTATACCGGGCTGAATATCCTCAATACCACCGA
TATCAATATCTCCACCGCCGAAGACCCGGTGGAGATCAATATGGAAGGTATCAACCAGGTCAACGTGAATAACCGCCAGG
GCCTGGACTTTGCCCAGGCGTTGCGCTCGTTCCTGCGTCAGGACCCGGACGTGATCATGGTGGGTGAGATCCGCGACCTG
GAAACTGCGGAGATCGCGATCAAGGCCGCGCAAACGGGGCACTTGGTATTGTCGACGCTGCACACCAACAGCGCCGCGGA
AACCCTTACCCGTTTGCAGAACATGGGCGTGGCCAGTTTCAACATTGCCACCTCGGTGAACTTGATCATTGCCCAGCGGC
TGGCGCGTAAGTTGTGCGGCCTGTGCAAGAAAGAACTGGAACTGCCCCGTGAAGTGCAGATCGAGGAAGGCTTCCCCGAG
GCGCAGATTGGCACGTTCAAGATTTACCAGCCCGTGGGTTGTGACCAATGCAACGGCGGCTATAAAGGCCGTCAGGGTAT
CTATGAAGTCGTGAAAGTGACCAAAGAGTTGCAGCGCCTGATCATGGAGTCGGGCAACTCGATTGAGATCGCTGCGCAAA
TGCGCAAAGAGGGCTTCAATGACCTGCGCACCTCCGGGCTGGTCAAAGTAATGCAAGGCGTCACCAGCCTGGCTGAAGTT
AACCGCGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.123

100

0.595
  pilB Acinetobacter baumannii D1279779

58.554

100

0.587
  pilB Legionella pneumophila strain ERS1305867

56.106

99.823

0.56
  pilB Vibrio cholerae strain A1552

51.327

99.823

0.512
  pilF Neisseria gonorrhoeae MS11

50.088

99.823

0.5
  pilB Vibrio campbellii strain DS40M4

49.735

100

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

50.906

97.527

0.496
  pilF Thermus thermophilus HB27

37.279

100

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

37.658

98.057

0.369