Detailed information NTG0822651

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QCD61_RS10440
• Genome accession: NZ_CP123771
• Coordinates: 2319152..2320261 (+)
• Length: 369 a.a.
• NCBI ID: WP_135844731.1
• Uniprot ID: -
• Organism: Pseudomonas viciae strain YsS1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2314152..2325261

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QCD61_RS10425 (QCD61_10425)	-	2315158..2316075 (+)	918	WP_256452555.1	sugar kinase	-
QCD61_RS10430 (QCD61_10430)	-	2316157..2316432 (-)	276	WP_003182808.1	peptidylprolyl isomerase	-
QCD61_RS10435 (QCD61_10435)	-	2316481..2319018 (-)	2538	WP_280944829.1	PAS domain-containing protein	-
QCD61_RS10440 (QCD61_10440)	pilU	2319152..2320261 (+)	1110	WP_135844731.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
QCD61_RS10445 (QCD61_10445)	-	2320587..2321936 (-)	1350	WP_280944830.1	FAD-dependent oxidoreductase	-
QCD61_RS10450 (QCD61_10450)	-	2322399..2324354 (-)	1956	WP_280944831.1	acetoacetate--CoA ligase	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 41143.14 Da
• Isoelectric Point: 6.2905

>NTDB_id=822651 QCD61_RS10440 WP_135844731.1 2319152..2320261(+) (pilU) [Pseudomonas viciae strain YsS1]
MEIDPLLRILASQDGSDLYMSTGAPPCVRFEGVLKPLGNQAFKVGDIAGLAESLMDAEQRLEFDRELEMNLAISMAGVGR
FRVNIFKQRNDVSMVIRNVKLDIPRFEDLKLPLVLLDTIMQKQGLILFVGATGSGKSTSLAALIDYRNRNSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTRTGQRRAAVEVMLGSPTVADLIRSNQFSELKGIMEKSVEQGMQTFDHAL
YALVVEGVIEEEEALKHADSVNNLRLQLKLHAEASPQPQAPSGEWGLMD

Nucleotide

• Length: 1110 bp

>NTDB_id=822651 QCD61_RS10440 WP_135844731.1 2319152..2320261(+) (pilU) [Pseudomonas viciae strain YsS1]
ATGGAAATCGATCCCTTGTTGCGAATCCTGGCAAGCCAGGATGGCTCCGACCTCTACATGTCCACCGGGGCGCCACCGTG
TGTGCGGTTCGAGGGCGTGCTCAAGCCCTTGGGCAACCAGGCATTCAAGGTCGGCGACATTGCCGGGCTCGCCGAGTCTT
TGATGGACGCCGAACAGCGCCTCGAGTTCGATCGGGAGCTGGAAATGAACCTGGCGATCTCCATGGCCGGTGTCGGGCGG
TTCCGGGTCAATATATTCAAGCAACGCAACGATGTGTCCATGGTGATACGCAACGTCAAGCTGGACATTCCGCGTTTCGA
AGACTTGAAATTGCCGCTGGTGTTACTGGACACCATTATGCAGAAGCAAGGGCTGATACTGTTCGTTGGCGCGACAGGGT
CGGGCAAGTCGACCTCCCTGGCGGCCCTGATCGATTACCGCAATCGCAACAGCAGCGGCCACATCATCACCATCGAAGAC
CCGGTGGAGTATATCCATCGGCATAAGAAATCGATCATCAACCAGCGCGAAGTCGGTGTCGACACCCGCAGTTTTCATGC
GGCCCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGGTGCTGATCGGCGAAATCCGTGATCGTGAAACCATGGAACACG
CCCTGGCCTTCGCCGACACCGGGCACCTGGTGATTTCCACGCTGCATGCCCACAACGCCAACCAGGCCCTGGACCGGGTG
ATCAATTTCTTCCCTGAGGAGCGCCGGCCGCAACTGCTGAACGATCTGGGCAACAACCTCAAGGCATTCGTCTCCCAGCG
CCTGGTGCGCACCCGCACCGGTCAGCGCCGGGCGGCGGTGGAGGTGATGCTGGGCTCGCCCACGGTGGCCGACCTGATCA
GGAGCAATCAGTTTTCTGAACTCAAGGGGATTATGGAAAAGTCCGTGGAGCAAGGGATGCAAACGTTCGACCACGCGCTT
TACGCGCTGGTCGTCGAGGGCGTGATTGAAGAGGAGGAAGCACTGAAGCATGCGGACTCGGTGAACAACCTGCGGCTGCA
GTTGAAATTGCACGCCGAAGCATCACCGCAGCCCCAAGCGCCGTCGGGCGAATGGGGCCTGATGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (80-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.056	96.206	0.539
pilU	Acinetobacter baylyi ADP1	51.821	96.748	0.501
pilU	Vibrio cholerae strain A1552	51.714	94.851	0.491
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.24	90.244	0.363
pilT	Vibrio cholerae strain A1552	40.24	90.244	0.363
pilT	Pseudomonas stutzeri DSM 10701	38.953	93.225	0.363
pilT	Pseudomonas aeruginosa PAK	38.663	93.225	0.36
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.233	91.87	0.36
pilT	Acinetobacter nosocomialis M2	39.82	90.515	0.36
pilT	Acinetobacter baumannii strain A118	39.82	90.515	0.36
pilT	Acinetobacter baumannii D1279779	39.82	90.515	0.36