Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   R3J27_RS11550 Genome accession   NZ_CP136979
Coordinates   2423098..2423532 (+) Length   144 a.a.
NCBI ID   WP_004084597.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. multiplex strain CFBP8418     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2418098..2428532
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R3J27_RS11530 (R3J27_11520) coaE 2418196..2418819 (-) 624 WP_004084593.1 dephospho-CoA kinase -
  R3J27_RS11535 (R3J27_11525) pilD 2418831..2419694 (-) 864 WP_004084594.1 A24 family peptidase Machinery gene
  R3J27_RS11540 (R3J27_11530) pilC 2419701..2420903 (-) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene
  R3J27_RS11545 (R3J27_11535) pilA 2421344..2421790 (+) 447 WP_004084596.1 pilin Machinery gene
  R3J27_RS11550 (R3J27_11540) pilA/pilAI 2423098..2423532 (+) 435 WP_004084597.1 pilin Machinery gene
  R3J27_RS11555 (R3J27_11545) pilB 2424372..2426105 (+) 1734 WP_004084598.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R3J27_RS11560 (R3J27_11550) - 2426199..2427611 (-) 1413 WP_020851962.1 sigma-54 dependent transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 14980.38 Da        Isoelectric Point: 8.5024

>NTDB_id=817206 R3J27_RS11550 WP_004084597.1 2423098..2423532(+) (pilA/pilAI) [Xylella fastidiosa subsp. multiplex strain CFBP8418]
MKKQQGFTLIELMIVIAIIAILAAIALPMYQNYVARSQVTAGLAEITPSKAQAEIRIAEARPTTVSTDIGLRTPTARCAV
AVDIKTDAESTITCTLIGNTQVNGQTIKLSRAVDNAAGTGGLWTCTTTVAEALAPGGCTSTKKS

Nucleotide


Download         Length: 435 bp        

>NTDB_id=817206 R3J27_RS11550 WP_004084597.1 2423098..2423532(+) (pilA/pilAI) [Xylella fastidiosa subsp. multiplex strain CFBP8418]
ATGAAAAAGCAACAAGGCTTCACTTTGATCGAGCTGATGATCGTCATTGCGATCATTGCCATCCTGGCAGCGATCGCTCT
GCCCATGTACCAAAATTATGTCGCCAGGTCTCAAGTCACAGCGGGACTGGCCGAAATTACACCCAGCAAAGCACAAGCCG
AAATCCGTATTGCCGAGGCAAGACCAACAACTGTTTCGACCGATATCGGACTGCGCACCCCAACGGCTCGTTGCGCCGTT
GCTGTCGACATCAAGACCGATGCTGAAAGCACAATTACATGTACGCTTATTGGTAATACCCAAGTCAACGGTCAAACCAT
TAAGTTGAGTCGCGCCGTCGATAACGCCGCAGGCACAGGGGGTCTGTGGACCTGTACCACAACCGTTGCCGAAGCGTTGG
CACCAGGAGGCTGCACTTCGACCAAGAAGAGCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.183

98.611

0.465
  pilA/pilAII Pseudomonas stutzeri DSM 10701

47.143

97.222

0.458
  pilA Pseudomonas aeruginosa PAK

41.026

100

0.444
  pilA Acinetobacter baumannii strain A118

44.444

100

0.444
  pilA Vibrio cholerae strain A1552

39.49

100

0.431
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.49

100

0.431
  pilA Vibrio cholerae C6706

39.49

100

0.431
  pilA Vibrio parahaemolyticus RIMD 2210633

42.143

97.222

0.41
  pilA Ralstonia pseudosolanacearum GMI1000

37.179

100

0.403
  comP Acinetobacter baylyi ADP1

35.714

100

0.382