Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   R3J27_RS11545 Genome accession   NZ_CP136979
Coordinates   2421344..2421790 (+) Length   148 a.a.
NCBI ID   WP_004084596.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. multiplex strain CFBP8418     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2416344..2426790
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R3J27_RS11525 (R3J27_11515) - 2416592..2417929 (+) 1338 WP_027700147.1 HAMP domain-containing sensor histidine kinase -
  R3J27_RS11530 (R3J27_11520) coaE 2418196..2418819 (-) 624 WP_004084593.1 dephospho-CoA kinase -
  R3J27_RS11535 (R3J27_11525) pilD 2418831..2419694 (-) 864 WP_004084594.1 A24 family peptidase Machinery gene
  R3J27_RS11540 (R3J27_11530) pilC 2419701..2420903 (-) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene
  R3J27_RS11545 (R3J27_11535) pilA 2421344..2421790 (+) 447 WP_004084596.1 pilin Machinery gene
  R3J27_RS11550 (R3J27_11540) pilA/pilAI 2423098..2423532 (+) 435 WP_004084597.1 pilin Machinery gene
  R3J27_RS11555 (R3J27_11545) pilB 2424372..2426105 (+) 1734 WP_004084598.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15505.75 Da        Isoelectric Point: 8.1062

>NTDB_id=817205 R3J27_RS11545 WP_004084596.1 2421344..2421790(+) (pilA) [Xylella fastidiosa subsp. multiplex strain CFBP8418]
MKKQQGFNLIELMIVIAIIAVLAAIALPMYQNYVARSQLTAALADITPGKVQAESLIADGKGTSNASDIGLRTDTTRCGI
TVEVDIAGTANITCKIKGNSQVKDKTIAWDRTPDNSAGTNGVNNGGVWTCYSNVTSDALRPSGCMAAK

Nucleotide


Download         Length: 447 bp        

>NTDB_id=817205 R3J27_RS11545 WP_004084596.1 2421344..2421790(+) (pilA) [Xylella fastidiosa subsp. multiplex strain CFBP8418]
ATGAAAAAGCAACAAGGTTTTAACTTAATCGAACTAATGATCGTCATTGCAATCATTGCTGTTCTGGCGGCCATCGCTCT
GCCCATGTACCAAAATTATGTTGCCAGATCCCAACTCACTGCGGCACTTGCCGACATCACGCCGGGCAAAGTGCAAGCCG
AGTCTCTGATTGCTGATGGGAAGGGTACATCCAACGCGAGTGATATCGGTCTGCGTACCGACACCACTCGTTGTGGAATT
ACCGTTGAAGTCGATATCGCTGGCACTGCCAACATCACATGCAAAATTAAGGGCAACTCTCAAGTCAAGGATAAAACCAT
CGCTTGGGATCGTACCCCCGATAACTCTGCAGGCACCAATGGCGTCAATAATGGAGGTGTGTGGACCTGCTACTCCAACG
TCACCTCCGATGCACTGAGACCTTCCGGCTGCATGGCTGCTAAATAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-144)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae strain A1552

40.385

100

0.426
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.385

100

0.426
  pilA Vibrio cholerae C6706

40.385

100

0.426
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.857

99.324

0.426
  pilA Pseudomonas aeruginosa PAK

38.994

100

0.419
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.816

99.324

0.405
  pilA Acinetobacter baumannii strain A118

38.667

100

0.392