Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   R3J29_RS09650 Genome accession   NZ_CP136974
Coordinates   2167350..2167793 (+) Length   147 a.a.
NCBI ID   WP_140189469.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. multiplex strain CFBP8075     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2162350..2172793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R3J29_RS09625 (R3J29_09610) coaE 2162451..2163074 (-) 624 WP_027700146.1 dephospho-CoA kinase -
  R3J29_RS09630 (R3J29_09615) pilD 2163086..2163949 (-) 864 WP_004084594.1 A24 family peptidase Machinery gene
  R3J29_RS09635 (R3J29_09620) pilC 2163956..2165158 (-) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene
  R3J29_RS09640 (R3J29_09625) pilA 2165599..2166045 (+) 447 WP_071869805.1 pilin Machinery gene
  R3J29_RS09645 (R3J29_09630) - 2166290..2166517 (-) 228 WP_012338102.1 hypothetical protein -
  R3J29_RS09650 (R3J29_09635) pilA/pilAI 2167350..2167793 (+) 444 WP_140189469.1 pilin Machinery gene
  R3J29_RS09655 (R3J29_09640) pilB 2168628..2170361 (+) 1734 WP_004084598.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R3J29_RS09660 (R3J29_09645) - 2170455..2171867 (-) 1413 WP_020851962.1 sigma-54 dependent transcriptional regulator -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15093.32 Da        Isoelectric Point: 8.1165

>NTDB_id=817087 R3J29_RS09650 WP_140189469.1 2167350..2167793(+) (pilA/pilAI) [Xylella fastidiosa subsp. multiplex strain CFBP8075]
MKKQQGFTLIELMIVIAIIAILAAIALPMYQNYVARSQVAAALAEITPGRTQAEIRMADGQASNTPNAIGLRTPTARCSL
VTVDITTTGGTIVCTMVGNGQVNNQTITLTRIADNNAGQGGVNTGGNWTCTTTAPAALTPAGCTGTN

Nucleotide


Download         Length: 444 bp        

>NTDB_id=817087 R3J29_RS09650 WP_140189469.1 2167350..2167793(+) (pilA/pilAI) [Xylella fastidiosa subsp. multiplex strain CFBP8075]
ATGAAAAAGCAACAAGGCTTCACTTTGATCGAGCTGATGATCGTCATTGCGATCATTGCCATCCTGGCCGCGATCGCTCT
GCCGATGTATCAGAATTATGTCGCCAGGTCTCAAGTCGCAGCGGCGCTGGCCGAGATCACACCGGGCCGAACACAAGCCG
AAATCCGTATGGCTGATGGACAGGCTTCAAACACTCCTAACGCTATTGGATTACGCACCCCGACCGCTCGTTGCAGCCTC
GTTACGGTCGACATCACAACTACTGGCGGCACAATCGTATGTACGATGGTTGGTAATGGTCAGGTCAACAATCAAACTAT
CACCTTGACCCGCATCGCTGATAACAACGCAGGCCAAGGTGGCGTCAATACTGGGGGGAACTGGACCTGTACCACAACCG
CGCCTGCAGCGTTAACTCCAGCAGGCTGCACTGGAACTAATTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-144)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.578

100

0.456
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.466

99.32

0.422
  pilA Vibrio cholerae strain A1552

38.509

100

0.422
  pilA Vibrio cholerae C6706

38.509

100

0.422
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.509

100

0.422
  pilA Pseudomonas aeruginosa PAK

38.365

100

0.415
  pilA Acinetobacter baumannii strain A118

39.865

100

0.401
  pilA Vibrio parahaemolyticus RIMD 2210633

38.621

98.639

0.381
  comP Acinetobacter baylyi ADP1

35.897

100

0.381
  pilA Haemophilus influenzae Rd KW20

36.486

100

0.367
  pilA Haemophilus influenzae 86-028NP

36.552

98.639

0.361