Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   R1T10_RS00255 Genome accession   NZ_CP136807
Coordinates   46491..46949 (+) Length   152 a.a.
NCBI ID   WP_002244388.1    Uniprot ID   -
Organism   Neisseria meningitidis NMB     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 41491..51949
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R1T10_RS00230 - 42616..43467 (-) 852 WP_002244390.1 RNA polymerase sigma-54 factor -
  R1T10_RS00235 katA 43815..45329 (+) 1515 WP_002239455.1 catalase KatA -
  R1T10_RS00240 - 45405..45542 (+) 138 WP_153308413.1 hypothetical protein -
  R1T10_RS00245 - 45629..45826 (-) 198 WP_153308414.1 hypothetical protein -
  R1T10_RS00250 - 45950..46078 (-) 129 WP_256864603.1 hypothetical protein -
  R1T10_RS00255 pilE 46491..46949 (+) 459 WP_002244388.1 pilin Machinery gene
  R1T10_RS00260 - 47179..47466 (+) 288 WP_139619977.1 hypothetical protein -
  R1T10_RS00265 - 47503..48003 (+) 501 WP_002226858.1 transposase -
  R1T10_RS00270 - 48467..49033 (+) 567 WP_002220235.1 manganese efflux pump MntP family protein -
  R1T10_RS00275 - 49455..51491 (-) 2037 WP_002244386.1 M3 family metallopeptidase -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 15934.28 Da        Isoelectric Point: 9.3582

>NTDB_id=815810 R1T10_RS00255 WP_002244388.1 46491..46949(+) (pilE) [Neisseria meningitidis NMB]
MKAIQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQMSEALTLAEGQKSAVVEYYSDNGTFPANNTSAGIAASSTIT
GKYVSSVAVTAEASGNASIAKITAKMKKNGVNKDISEKTLVLVGKQNSGSFSWTCQKGDVDEKFLPSSCRAK

Nucleotide


Download         Length: 459 bp        

>NTDB_id=815810 R1T10_RS00255 WP_002244388.1 46491..46949(+) (pilE) [Neisseria meningitidis NMB]
ATGAAAGCAATCCAAAAAGGTTTCACCCTGATCGAGCTGATGATCGTCATCGCCATCGTCGGTATCTTGGCAGCCGTCGC
CCTGCCCGCATACCAAGACTACACCGCGCGCGCCCAAATGTCCGAAGCCCTGACTTTGGCGGAAGGTCAAAAATCCGCAG
TGGTCGAGTATTATTCCGACAACGGTACGTTCCCTGCCAACAATACTTCCGCAGGTATTGCTGCTTCTAGTACAATTACA
GGTAAATATGTGAGTTCTGTAGCAGTTACAGCGGAAGCATCTGGCAATGCATCTATTGCCAAGATTACCGCCAAAATGAA
AAAAAATGGTGTGAACAAAGATATTTCAGAAAAAACTTTGGTGCTGGTGGGTAAACAAAACAGTGGTTCGTTCTCTTGGA
CTTGTCAAAAAGGCGATGTGGACGAAAAATTCCTGCCGTCTTCCTGCCGCGCCAAATAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Pilin (bacterial filament) (Pilin)

(36-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

57.229

100

0.625
  pilE Neisseria gonorrhoeae MS11

53.846

100

0.599
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.458

100

0.461
  pilA2 Legionella pneumophila strain ERS1305867

44.295

98.026

0.434
  pilA2 Legionella pneumophila str. Paris

43.333

98.684

0.428
  comP Acinetobacter baylyi ADP1

41.176

100

0.414
  pilA/pilA1 Eikenella corrodens VA1

38.994

100

0.408
  pilA Ralstonia pseudosolanacearum GMI1000

37.126

100

0.408
  pilA Acinetobacter baumannii strain A118

40.132

100

0.401
  pilA Acinetobacter nosocomialis M2

38.667

98.684

0.382
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.538

100

0.375