Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QC826_RS24495 Genome accession   NZ_CP122407
Coordinates   5318647..5319834 (-) Length   395 a.a.
NCBI ID   WP_279762845.1    Uniprot ID   -
Organism   Rugamonas sp. DEMB1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5313647..5324834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QC826_RS24470 (QC826_24470) coxB 5314019..5315245 (-) 1227 WP_279762840.1 cytochrome c oxidase subunit II -
  QC826_RS24475 (QC826_24475) - 5315703..5316635 (-) 933 WP_279762841.1 methyltransferase domain-containing protein -
  QC826_RS24480 (QC826_24480) - 5316694..5317551 (+) 858 WP_279762842.1 ComF family protein -
  QC826_RS24485 (QC826_24485) trmL 5317626..5318096 (+) 471 WP_279762843.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
  QC826_RS24490 (QC826_24490) rfaE2 5318142..5318615 (-) 474 WP_279762844.1 D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase -
  QC826_RS24495 (QC826_24495) pilU 5318647..5319834 (-) 1188 WP_279762845.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QC826_RS24500 (QC826_24500) - 5320016..5320954 (+) 939 WP_279762846.1 hydrogen peroxide-inducible genes activator -
  QC826_RS24505 (QC826_24505) ubiA 5321062..5321917 (+) 856 Protein_4852 4-hydroxybenzoate octaprenyltransferase -
  QC826_RS24510 (QC826_24510) - 5322011..5322376 (+) 366 WP_279762847.1 YqjD family protein -
  QC826_RS24515 (QC826_24515) - 5322508..5323302 (+) 795 WP_279762848.1 substrate-binding periplasmic protein -
  QC826_RS24520 (QC826_24520) proC 5323320..5324156 (-) 837 WP_279762849.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 395 a.a.        Molecular weight: 43781.29 Da        Isoelectric Point: 6.6879

>NTDB_id=815480 QC826_RS24495 WP_279762845.1 5318647..5319834(-) (pilU) [Rugamonas sp. DEMB1]
MSATFGPDEAQAYLHKLLKVMHHMGGSDLFISADFPPSIKSQGAMKPLSAQRLSGEVTRALALSIMNDKQRREFEAELEC
NFAISLPEVCRFRVNVFVQQQCVAMVIRTIASEIPNFEKLELPEVLKEVIMTKRGLVLVVGGTGSGKSTTLAAMIDYRNS
NSAGHIITVEGPVEYVHKNKGCLITHREVGVDTLSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQTMDRIINFFPEERRNQLLMDLSSNLRAIVSQRLVRTEDGKGRKAAIEILLNTPTIAEMIFKGNFQAIKEIMHKS
RELGMCTFDQALYELYNRGHIAYEEAIRNADSANGLRLQIKLRGERKEPGDAGPAAASALSMQIEAEPEPEPPGH

Nucleotide


Download         Length: 1188 bp        

>NTDB_id=815480 QC826_RS24495 WP_279762845.1 5318647..5319834(-) (pilU) [Rugamonas sp. DEMB1]
ATGTCCGCCACCTTTGGCCCCGACGAGGCCCAAGCCTATCTGCACAAGCTGCTCAAGGTGATGCACCACATGGGCGGCTC
CGACTTGTTCATCTCCGCCGACTTCCCGCCCAGTATCAAGTCGCAAGGGGCGATGAAGCCGCTCAGCGCGCAACGCCTGA
GCGGCGAGGTGACGCGCGCGCTGGCCCTGTCCATCATGAACGACAAGCAGCGCCGCGAGTTCGAGGCCGAGCTCGAATGC
AACTTCGCCATTTCGCTGCCCGAGGTGTGCCGCTTCCGCGTCAACGTCTTCGTCCAGCAGCAGTGCGTGGCCATGGTGAT
CCGCACCATCGCCTCGGAGATCCCGAATTTCGAGAAGCTCGAACTGCCCGAGGTGTTGAAGGAGGTCATCATGACCAAGC
GCGGCCTGGTGCTGGTGGTGGGCGGCACCGGCTCGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACCGCAACAGC
AACTCGGCCGGCCACATCATCACCGTCGAGGGCCCGGTCGAGTACGTGCACAAGAACAAGGGCTGCCTGATCACCCACCG
CGAGGTCGGCGTCGACACCCTGTCCTGGCACAACGCGCTGAAAAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATCG
GCGAGATCCGCGACACCGAGACCATGGAGCACGCCATCGCCTTCGCCGAGACGGGCCACCTGTGCCTGGGCACGCTGCAC
GCCAACAACGCCAACCAGACCATGGACCGCATCATCAACTTCTTCCCCGAGGAGCGGCGCAACCAGTTGCTGATGGACTT
GTCGTCGAACCTGCGCGCCATCGTCTCGCAGCGCCTGGTGCGCACCGAGGACGGCAAGGGCCGCAAGGCGGCCATCGAGA
TTTTGCTGAACACGCCGACCATCGCCGAGATGATTTTCAAGGGCAATTTCCAGGCGATCAAGGAAATCATGCACAAATCG
CGCGAGCTGGGCATGTGCACCTTCGACCAGGCGCTGTACGAACTCTATAATAGGGGCCATATCGCCTACGAGGAGGCGAT
CCGCAACGCCGATTCGGCCAACGGCCTGCGCCTGCAGATCAAGCTGCGCGGCGAGCGCAAGGAGCCCGGCGACGCCGGCC
CGGCGGCCGCCAGCGCGCTGAGCATGCAGATCGAGGCCGAGCCGGAGCCGGAACCGCCGGGCCACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(102-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.659

89.114

0.532
  pilU Acinetobacter baylyi ADP1

54.039

90.886

0.491
  pilU Vibrio cholerae strain A1552

48.387

94.177

0.456
  pilT Legionella pneumophila strain Lp02

44.985

83.291

0.375
  pilT Legionella pneumophila strain ERS1305867

44.985

83.291

0.375
  pilT Pseudomonas aeruginosa PAK

44.109

83.797

0.37
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.73

85.316

0.365
  pilT Pseudomonas stutzeri DSM 10701

42.687

84.81

0.362
  pilT Acinetobacter baumannii D1279779

44.41

81.519

0.362
  pilT Acinetobacter baumannii strain A118

44.41

81.519

0.362
  pilT Acinetobacter nosocomialis M2

44.41

81.519

0.362