Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   ORD27_RS08750 Genome accession   NZ_CP121233
Coordinates   1762556..1762831 (-) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain SVJ-EF01     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1720021..1780937 1762556..1762831 within 0


Gene organization within MGE regions


Location: 1720021..1780937
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ORD27_RS08455 (ORD27_008455) - 1720021..1721028 (-) 1008 WP_002357063.1 class I SAM-dependent methyltransferase -
  ORD27_RS08460 (ORD27_008460) comGG 1721157..1721510 (-) 354 WP_016618446.1 competence type IV pilus minor pilin ComGG -
  ORD27_RS08465 (ORD27_008465) comGF 1721510..1721944 (-) 435 WP_010707381.1 competence type IV pilus minor pilin ComGF -
  ORD27_RS08470 (ORD27_008470) - 1721934..1722305 (-) 372 WP_173962365.1 type II secretion system protein -
  ORD27_RS08475 (ORD27_008475) hemH 1723061..1724002 (-) 942 WP_002364185.1 ferrochelatase -
  ORD27_RS08485 (ORD27_008485) - 1724742..1724990 (-) 249 WP_002383136.1 hypothetical protein -
  ORD27_RS08490 (ORD27_008490) - 1725062..1725262 (-) 201 WP_002357053.1 cold-shock protein -
  ORD27_RS08495 (ORD27_008495) - 1726099..1727340 (-) 1242 WP_002370962.1 LysM peptidoglycan-binding domain-containing protein -
  ORD27_RS08500 (ORD27_008500) - 1727341..1727574 (-) 234 WP_002384371.1 phage holin -
  ORD27_RS08505 (ORD27_008505) - 1727567..1727788 (-) 222 WP_002364191.1 hypothetical protein -
  ORD27_RS08510 (ORD27_008510) - 1727823..1727978 (-) 156 WP_002364192.1 XkdX family protein -
  ORD27_RS08515 (ORD27_008515) - 1727980..1728300 (-) 321 WP_002389262.1 hypothetical protein -
  ORD27_RS08520 (ORD27_008520) - 1728314..1728805 (-) 492 WP_002364194.1 hypothetical protein -
  ORD27_RS08525 (ORD27_008525) - 1728805..1729056 (-) 252 WP_014524854.1 hypothetical protein -
  ORD27_RS08530 (ORD27_008530) - 1729053..1729649 (-) 597 WP_002357045.1 hypothetical protein -
  ORD27_RS08535 (ORD27_008535) - 1729642..1730523 (-) 882 WP_002387290.1 phage baseplate upper protein -
  ORD27_RS08540 (ORD27_008540) - 1730542..1733349 (-) 2808 WP_271865924.1 phage tail spike protein -
  ORD27_RS08545 (ORD27_008545) - 1733331..1734065 (-) 735 WP_271865926.1 phage tail protein -
  ORD27_RS08550 (ORD27_008550) - 1734055..1736952 (-) 2898 WP_002387288.1 tape measure protein -
  ORD27_RS08555 (ORD27_008555) - 1737200..1737550 (-) 351 WP_002357039.1 hypothetical protein -
  ORD27_RS08560 (ORD27_008560) - 1737603..1738451 (-) 849 WP_002387287.1 major tail protein -
  ORD27_RS08565 (ORD27_008565) - 1738452..1738826 (-) 375 WP_002387286.1 DUF6838 family protein -
  ORD27_RS08570 (ORD27_008570) - 1738829..1739227 (-) 399 WP_002357036.1 HK97 gp10 family phage protein -
  ORD27_RS08575 (ORD27_008575) - 1739220..1739594 (-) 375 WP_002387285.1 hypothetical protein -
  ORD27_RS08580 (ORD27_008580) - 1739591..1739935 (-) 345 WP_002387282.1 hypothetical protein -
  ORD27_RS08585 (ORD27_008585) - 1739949..1740131 (-) 183 WP_002387281.1 hypothetical protein -
  ORD27_RS08590 (ORD27_008590) - 1740160..1741047 (-) 888 WP_002387280.1 DUF5309 domain-containing protein -
  ORD27_RS08595 (ORD27_008595) - 1741061..1741684 (-) 624 WP_010706493.1 DUF4355 domain-containing protein -
  ORD27_RS08600 (ORD27_008600) - 1741903..1742222 (-) 320 Protein_1666 hypothetical protein -
  ORD27_RS08605 (ORD27_008605) - 1742279..1742509 (-) 231 WP_002380437.1 hypothetical protein -
  ORD27_RS08610 (ORD27_008610) - 1742510..1744414 (-) 1905 WP_010714050.1 SPP1 gp7 family phage head morphogenesis protein -
  ORD27_RS08615 (ORD27_008615) - 1744389..1745861 (-) 1473 WP_010706495.1 phage portal protein -
  ORD27_RS08620 (ORD27_008620) terL 1745873..1747261 (-) 1389 WP_002387276.1 phage terminase large subunit -
  ORD27_RS08625 (ORD27_008625) - 1747254..1747715 (-) 462 WP_002383816.1 helix-turn-helix domain-containing protein -
  ORD27_RS08630 (ORD27_008630) - 1747791..1747967 (-) 177 WP_010706496.1 hypothetical protein -
  ORD27_RS08635 (ORD27_008635) - 1748151..1748963 (-) 813 WP_002387275.1 hypothetical protein -
  ORD27_RS08640 (ORD27_008640) - 1749075..1749308 (-) 234 Protein_1674 hypothetical protein -
  ORD27_RS08645 (ORD27_008645) - 1749892..1750362 (-) 471 WP_002383811.1 hypothetical protein -
  ORD27_RS08650 (ORD27_008650) - 1750387..1751310 (-) 924 WP_002383810.1 DUF6731 family protein -
  ORD27_RS08660 (ORD27_008660) - 1752028..1752444 (-) 417 WP_010706497.1 ArpU family phage packaging/lysis transcriptional regulator -
  ORD27_RS08665 (ORD27_008665) - 1753352..1753760 (-) 409 Protein_1678 RusA family crossover junction endodeoxyribonuclease -
  ORD27_RS08670 (ORD27_008670) - 1753757..1754614 (-) 858 WP_002387271.1 helix-turn-helix domain-containing protein -
  ORD27_RS08675 (ORD27_008675) - 1754614..1754814 (-) 201 WP_002357010.1 hypothetical protein -
  ORD27_RS08680 (ORD27_008680) - 1754819..1755460 (-) 642 WP_002364344.1 putative HNHc nuclease -
  ORD27_RS08685 (ORD27_008685) - 1755465..1756199 (-) 735 WP_002383799.1 ERF family protein -
  ORD27_RS08690 (ORD27_008690) - 1756192..1756509 (-) 318 WP_002383798.1 hypothetical protein -
  ORD27_RS08695 (ORD27_008695) - 1756705..1757043 (-) 339 WP_002383796.1 hypothetical protein -
  ORD27_RS08700 (ORD27_008700) - 1757080..1757289 (-) 210 WP_002378465.1 hypothetical protein -
  ORD27_RS08705 (ORD27_008705) - 1757344..1757532 (+) 189 WP_002357001.1 YegP family protein -
  ORD27_RS08710 (ORD27_008710) - 1757558..1758280 (-) 723 WP_002387270.1 phage regulatory protein -
  ORD27_RS08715 (ORD27_008715) - 1758303..1758614 (-) 312 WP_002403885.1 hypothetical protein -
  ORD27_RS08720 (ORD27_008720) - 1758626..1758817 (-) 192 WP_002383936.1 hypothetical protein -
  ORD27_RS08725 (ORD27_008725) - 1759113..1759454 (+) 342 WP_002387267.1 helix-turn-helix transcriptional regulator -
  ORD27_RS08730 (ORD27_008730) - 1759459..1760109 (+) 651 WP_002387266.1 ImmA/IrrE family metallo-endopeptidase -
  ORD27_RS08735 (ORD27_008735) - 1760205..1760834 (+) 630 WP_002387265.1 SHOCT domain-containing protein -
  ORD27_RS08740 (ORD27_008740) - 1760940..1762088 (+) 1149 WP_002387264.1 site-specific integrase -
  ORD27_RS08745 (ORD27_008745) comGD 1762125..1762559 (-) 435 Protein_1694 competence type IV pilus minor pilin ComGD -
  ORD27_RS08750 (ORD27_008750) comGC/cglC 1762556..1762831 (-) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene
  ORD27_RS08755 (ORD27_008755) comGB 1762831..1763877 (-) 1047 WP_104846628.1 competence type IV pilus assembly protein ComGB -
  ORD27_RS08760 (ORD27_008760) comGA 1763834..1764802 (-) 969 WP_085443259.1 competence type IV pilus ATPase ComGA -
  ORD27_RS08765 (ORD27_008765) - 1765042..1766370 (-) 1329 WP_064687249.1 amino acid permease -
  ORD27_RS08770 (ORD27_008770) rlmN 1766660..1767733 (-) 1074 WP_002356987.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ORD27_RS08775 (ORD27_008775) - 1767859..1769688 (-) 1830 WP_010713274.1 ABC transporter permease -
  ORD27_RS08780 (ORD27_008780) - 1769678..1770427 (-) 750 WP_002381711.1 ABC transporter ATP-binding protein -
  ORD27_RS08785 (ORD27_008785) - 1770545..1771246 (-) 702 WP_002356983.1 GntR family transcriptional regulator -
  ORD27_RS08790 (ORD27_008790) - 1771377..1773800 (-) 2424 WP_070415770.1 DNA translocase FtsK -
  ORD27_RS08795 (ORD27_008795) - 1774114..1775325 (-) 1212 WP_002401320.1 NAD(P)/FAD-dependent oxidoreductase -
  ORD27_RS08800 (ORD27_008800) - 1775354..1776259 (-) 906 WP_002360016.1 prenyltransferase -
  ORD27_RS08805 (ORD27_008805) - 1776381..1777361 (+) 981 WP_002360015.1 polyprenyl synthetase family protein -
  ORD27_RS08810 (ORD27_008810) cydC 1777441..1779207 (-) 1767 WP_085443342.1 thiol reductant ABC exporter subunit CydC -
  ORD27_RS08815 (ORD27_008815) cydD 1779204..1780937 (-) 1734 WP_002364369.1 thiol reductant ABC exporter subunit CydD -

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=811275 ORD27_RS08750 WP_002356991.1 1762556..1762831(-) (comGC/cglC) [Enterococcus faecalis strain SVJ-EF01]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=811275 ORD27_RS08750 WP_002356991.1 1762556..1762831(-) (comGC/cglC) [Enterococcus faecalis strain SVJ-EF01]
ATGAAAAAGAAACAAAAATACGCGGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTCCCTAACTTAGCGAAACATAAAGAAACAGTTGACAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCCTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396