Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ROT04_RS24595 Genome accession   NZ_CP135174
Coordinates   5252019..5252483 (-) Length   154 a.a.
NCBI ID   WP_033970663.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PARM_L1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5247019..5257483
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ROT04_RS24580 (ROT04_24580) nadC 5249519..5250367 (+) 849 WP_058355295.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ROT04_RS24590 (ROT04_24590) - 5250548..5251945 (-) 1398 WP_236664812.1 O-antigen ligase family protein -
  ROT04_RS24595 (ROT04_24595) pilA 5252019..5252483 (-) 465 WP_033970663.1 pilin Machinery gene
  ROT04_RS24600 (ROT04_24600) pilB 5252714..5254414 (+) 1701 WP_058355294.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ROT04_RS24605 (ROT04_24605) pilC 5254418..5255635 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  ROT04_RS24610 (ROT04_24610) pilD 5255636..5256508 (+) 873 WP_058355293.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ROT04_RS24615 (ROT04_24615) coaE 5256505..5257116 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  ROT04_RS24620 (ROT04_24620) yacG 5257113..5257313 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16009.27 Da        Isoelectric Point: 9.0066

>NTDB_id=810382 ROT04_RS24595 WP_033970663.1 5252019..5252483(-) (pilA) [Pseudomonas aeruginosa strain PARM_L1]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSSDTPGNNEYDLGFTS
STLLTGSGKGQIKIDKADTATPEISGTLGNSSGKGIAGAVITVKRDDKGVWTCGISGSPTNWKTNYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=810382 ROT04_RS24595 WP_033970663.1 5252019..5252483(-) (pilA) [Pseudomonas aeruginosa strain PARM_L1]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTCGTTTCCAGCGATACCCCCGGAAACAATGAATATGATCTTGGCTTTACCAGC
TCTACTCTGCTTACTGGTAGCGGTAAGGGGCAGATCAAGATTGACAAAGCTGATACCGCAACTCCGGAGATTTCTGGTAC
TCTGGGCAACTCTTCTGGTAAGGGTATCGCTGGCGCTGTCATCACTGTCAAGCGTGATGATAAAGGAGTATGGACCTGCG
GCATCAGCGGTTCGCCGACCAACTGGAAAACCAACTACGCCCCGGCCAACTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-151)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

44.586

100

0.455
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.871

100

0.442
  pilA Pseudomonas aeruginosa PAK

41.139

100

0.422
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.447

98.701

0.409
  pilA Vibrio cholerae strain A1552

41.447

98.701

0.409
  pilA Vibrio cholerae C6706

41.447

98.701

0.409
  pilA Ralstonia pseudosolanacearum GMI1000

36.471

100

0.403
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.94

96.753

0.396