Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ROT04_RS24600 Genome accession   NZ_CP135174
Coordinates   5252714..5254414 (+) Length   566 a.a.
NCBI ID   WP_058355294.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PARM_L1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5247714..5259414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ROT04_RS24580 (ROT04_24580) nadC 5249519..5250367 (+) 849 WP_058355295.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ROT04_RS24590 (ROT04_24590) - 5250548..5251945 (-) 1398 WP_236664812.1 O-antigen ligase family protein -
  ROT04_RS24595 (ROT04_24595) pilA 5252019..5252483 (-) 465 WP_033970663.1 pilin Machinery gene
  ROT04_RS24600 (ROT04_24600) pilB 5252714..5254414 (+) 1701 WP_058355294.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ROT04_RS24605 (ROT04_24605) pilC 5254418..5255635 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  ROT04_RS24610 (ROT04_24610) pilD 5255636..5256508 (+) 873 WP_058355293.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ROT04_RS24615 (ROT04_24615) coaE 5256505..5257116 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  ROT04_RS24620 (ROT04_24620) yacG 5257113..5257313 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  ROT04_RS24625 (ROT04_24625) - 5257350..5257559 (-) 210 WP_058355292.1 hypothetical protein -
  ROT04_RS24630 (ROT04_24630) - 5257665..5258354 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  ROT04_RS24635 (ROT04_24635) - 5258351..5258821 (-) 471 WP_003094664.1 hypothetical protein -
  ROT04_RS24640 (ROT04_24640) - 5258818..5259243 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62356.26 Da        Isoelectric Point: 5.4489

>NTDB_id=810383 ROT04_RS24600 WP_058355294.1 5252714..5254414(+) (pilB) [Pseudomonas aeruginosa strain PARM_L1]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
VISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQEDSGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKNKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=810383 ROT04_RS24600 WP_058355294.1 5252714..5254414(+) (pilB) [Pseudomonas aeruginosa strain PARM_L1]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGT
TGGTCGAGGTGGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GTCATCAGCGAGCGCCTGGTCCGCCAGCACCGCGTGTTGCCGCTGTGGAAGCGCGGCAACAAACTGTTCATCGGCCTCTC
CGACCCGACCAATCACCAAGCGGTCACCGATATCCAGTTCAGCACCGGTCTCACCACCGAAGCCCTGCTGGTGGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTCTACGAGGGCGCCATGGACGCCCTCGGCGACCTCGGCGATATCGATCTCGAC
GGAGTCGATGTGGGCAAGGCAGAAAGCGCCAGCCAGGAAGATTCCGGCGACGACGCCGAAGATGCCCCGGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAACCCTACGAAAAGACCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCAAAGAACAA
GTCGATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCCCTCGGCTACGAAGAGGATCAGAAGGAACTTTATCTGGCCGCGCTCAAACAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGCCTGAACATCCTCAATACCACCGA
CATCAACATCTCCACTGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTACGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATCGCCATCAAGGCGGCGCAAACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAAGGTTTCCCCGAA
GAGCTGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.719

100

0.581
  pilB Acinetobacter baylyi ADP1

56.614

100

0.567
  pilB Legionella pneumophila strain ERS1305867

54.641

100

0.551
  pilB Vibrio cholerae strain A1552

51.699

98.763

0.511
  pilF Neisseria gonorrhoeae MS11

50.355

99.647

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

52.345

94.17

0.493
  pilB Vibrio campbellii strain DS40M4

51.407

94.17

0.484
  pilB/pilB1 Synechocystis sp. PCC 6803

37.763

100

0.412
  pilF Thermus thermophilus HB27

38.84

100

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.909

97.173

0.378