Detailed information NTG0810385

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ROT04_RS24610
• Genome accession: NZ_CP135174
• Coordinates: 5255636..5256508 (+)
• Length: 290 a.a.
• NCBI ID: WP_058355293.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain PARM_L1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5250636..5261508

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ROT04_RS24595 (ROT04_24595)	pilA	5252019..5252483 (-)	465	WP_033970663.1	pilin	Machinery gene
ROT04_RS24600 (ROT04_24600)	pilB	5252714..5254414 (+)	1701	WP_058355294.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ROT04_RS24605 (ROT04_24605)	pilC	5254418..5255635 (+)	1218	WP_003161763.1	type II secretion system F family protein	Machinery gene
ROT04_RS24610 (ROT04_24610)	pilD	5255636..5256508 (+)	873	WP_058355293.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
ROT04_RS24615 (ROT04_24615)	coaE	5256505..5257116 (+)	612	WP_003094654.1	dephospho-CoA kinase	-
ROT04_RS24620 (ROT04_24620)	yacG	5257113..5257313 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
ROT04_RS24625 (ROT04_24625)	-	5257350..5257559 (-)	210	WP_058355292.1	hypothetical protein	-
ROT04_RS24630 (ROT04_24630)	-	5257665..5258354 (-)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
ROT04_RS24635 (ROT04_24635)	-	5258351..5258821 (-)	471	WP_003094664.1	hypothetical protein	-
ROT04_RS24640 (ROT04_24640)	-	5258818..5259243 (-)	426	WP_003103865.1	GNAT family N-acetyltransferase	-
ROT04_RS24645 (ROT04_24645)	-	5259376..5260005 (+)	630	WP_003094668.1	DUF1780 domain-containing protein	-
ROT04_RS24650 (ROT04_24650)	-	5260002..5260451 (+)	450	WP_003094670.1	MOSC domain-containing protein	-
ROT04_RS24655 (ROT04_24655)	-	5260477..5260647 (+)	171	WP_003094672.1	DUF3094 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31857.91 Da
• Isoelectric Point: 7.7068

>NTDB_id=810385 ROT04_RS24610 WP_058355293.1 5255636..5256508(+) (pilD) [Pseudomonas aeruginosa strain PARM_L1]
MTLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLISYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=810385 ROT04_RS24610 WP_058355293.1 5255636..5256508(+) (pilD) [Pseudomonas aeruginosa strain PARM_L1]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTTGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATACCGCTGATCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTATGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGCCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGTCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterProScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.714	96.552	0.538
pilD	Vibrio campbellii strain DS40M4	53.357	97.586	0.521
pilD	Acinetobacter nosocomialis M2	50.883	97.586	0.497
pilD	Acinetobacter baumannii D1279779	50.883	97.586	0.497
pilD	Neisseria gonorrhoeae MS11	48.921	95.862	0.469