NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	P4833_RS27475	Genome accession	NZ_CP120871
Coordinates	5807563..5808015 (-)	Length	150 a.a.
NCBI ID	WP_024928732.1	Uniprot ID	-
Organism	Pseudomonas aeruginosa strain PACL
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5802563..5813015

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P4833_RS27455 (P4833_27415)	ampD	5803280..5803846 (-)	567	WP_003104911.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
P4833_RS27460 (P4833_27420)	-	5803997..5806252 (-)	2256	WP_003104913.1	DUF1631 domain-containing protein	-
P4833_RS27465 (P4833_27425)	nadC	5806538..5807386 (+)	849	WP_047688821.1	carboxylating nicotinate-nucleotide diphosphorylase	-
P4833_RS27475 (P4833_27435)	pilA	5807563..5808015 (-)	453	WP_024928732.1	pilin	Machinery gene
P4833_RS27480 (P4833_27440)	pilB	5808247..5809947 (+)	1701	WP_024928731.1	type IV-A pilus assembly ATPase PilB	Machinery gene
P4833_RS27485 (P4833_27445)	pilC	5809951..5811171 (+)	1221	WP_009315898.1	type 4a pilus biogenesis protein PilC	Machinery gene
P4833_RS27490 (P4833_27450)	pilD	5811175..5812047 (+)	873	WP_009875878.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
P4833_RS27495 (P4833_27455)	coaE	5812044..5812655 (+)	612	WP_003112838.1	dephospho-CoA kinase	-
P4833_RS27500 (P4833_27460)	yacG	5812652..5812852 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

Download Length: 150 a.a. Molecular weight: 15413.63 Da Isoelectric Point: 8.4829

                                >NTDB_id=809074 P4833_RS27475 WP_024928732.1 5807563..5808015(-) (pilA) [Pseudomonas aeruginosa strain PACL]

MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGENIKIGSQASTADTTYV

GVDATANKLGTIAVTIGNNGAGTVTFTFAAGNSSPKNSGKIITLTRTDAGSWACTSTQDEMFIPKGCKAP

Nucleotide

Download Length: 453 bp

                                >NTDB_id=809074 P4833_RS27475 WP_024928732.1 5807563..5808015(-) (pilA) [Pseudomonas aeruginosa strain PACL]

ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT

TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCACTGAAGACCACTGTTG

AAGAGTCGCTGTCGCGTGGAATCGCTGGGGAAAATATTAAAATTGGTTCCCAAGCATCTACTGCAGATACTACCTATGTA

GGTGTTGATGCTACCGCAAATAAGCTTGGCACTATTGCTGTGACAATTGGGAACAATGGCGCAGGTACCGTGACTTTCAC

TTTTGCAGCCGGCAATTCCAGTCCGAAAAACTCCGGTAAAATTATCACCCTGACCCGGACTGATGCAGGGTCGTGGGCTT

GTACTTCTACCCAGGACGAAATGTTCATCCCTAAAGGTTGCAAAGCGCCTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Pseudomonas aeruginosa PAK	68.456	99.333	0.68
pilA/pilAI	Pseudomonas stutzeri DSM 10701	47.333	100	0.473
pilA	Vibrio parahaemolyticus RIMD 2210633	46.939	98	0.46
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	43.671	100	0.46
pilA	Vibrio cholerae strain A1552	43.671	100	0.46
pilA	Vibrio cholerae C6706	43.671	100	0.46
pilA	Acinetobacter baumannii strain A118	44.295	99.333	0.44
comP	Acinetobacter baylyi ADP1	41.558	100	0.427
pilA2	Legionella pneumophila strain ERS1305867	42.282	99.333	0.42
pilA/pilAII	Pseudomonas stutzeri DSM 10701	43.357	95.333	0.413
pilA2	Legionella pneumophila str. Paris	41.611	99.333	0.413

Detailed information NTG0809074