Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   RG643_RS28370 Genome accession   NZ_CP133753
Coordinates   6040380..6040853 (+) Length   157 a.a.
NCBI ID   WP_309543270.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain ZYPA187     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6035380..6045853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG643_RS28345 (RG643_28340) yacG 6035548..6035748 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  RG643_RS28350 (RG643_28345) coaE 6035745..6036356 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  RG643_RS28355 (RG643_28350) pilD 6036353..6037225 (-) 873 WP_003116251.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  RG643_RS28360 (RG643_28355) pilC 6037228..6038445 (-) 1218 WP_044061094.1 type II secretion system F family protein Machinery gene
  RG643_RS28365 (RG643_28360) pilB 6038449..6040149 (-) 1701 WP_309543269.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RG643_RS28370 (RG643_28365) pilE 6040380..6040853 (+) 474 WP_309543270.1 pilin Machinery gene
  RG643_RS28380 (RG643_28375) nadC 6041006..6041854 (-) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RG643_RS28385 (RG643_28380) - 6042140..6044395 (+) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  RG643_RS28390 (RG643_28385) ampD 6044546..6045112 (+) 567 WP_003116245.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 16135.44 Da        Isoelectric Point: 7.5840

>NTDB_id=800845 RG643_RS28370 WP_309543270.1 6040380..6040853(+) (pilE) [Pseudomonas aeruginosa strain ZYPA187]
MKAAQKGFTLIELMIVVAIIGILAAIAIPAYQDYTARAQLSEAMTLASGLKTKVSDIFSQDGSCPANTAATAGIEKDTDI
NGKYVAKVTTGGTAAASGGCTIVATMKASDVATPLRETTLTLTLGNADKGSYTWACTSNADNKYLPKTCQTATTTTP

Nucleotide


Download         Length: 474 bp        

>NTDB_id=800845 RG643_RS28370 WP_309543270.1 6040380..6040853(+) (pilE) [Pseudomonas aeruginosa strain ZYPA187]
ATGAAAGCTGCTCAAAAAGGCTTTACCTTGATCGAATTGATGATCGTGGTCGCGATCATCGGTATTCTGGCTGCCATCGC
TATTCCAGCGTATCAGGACTACACCGCACGCGCTCAGCTTAGCGAAGCCATGACCCTGGCCAGTGGTCTCAAGACGAAAG
TGAGCGATATCTTCTCTCAGGATGGGTCCTGCCCGGCTAATACTGCTGCCACGGCAGGCATCGAGAAAGATACCGACATC
AACGGCAAGTATGTTGCCAAGGTAACAACTGGTGGCACCGCAGCTGCGTCTGGTGGTTGCACTATCGTTGCTACTATGAA
AGCCTCTGATGTGGCTACTCCTCTGAGGGAGACAACTCTGACTTTGACTCTAGGAAATGCTGACAAGGGTTCTTACACTT
GGGCCTGTACTTCCAACGCAGATAACAAGTACCTGCCAAAAACCTGCCAGACTGCTACCACTACCACTCCGTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(36-150)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

41.618

100

0.459
  comP Acinetobacter baylyi ADP1

46.405

97.452

0.452
  pilE Neisseria gonorrhoeae MS11

41.317

100

0.439
  pilA2 Legionella pneumophila str. Paris

44.156

98.089

0.433
  pilA/pilA1 Eikenella corrodens VA1

40.741

100

0.42
  pilA2 Legionella pneumophila strain ERS1305867

43.421

96.815

0.42
  pilA Ralstonia pseudosolanacearum GMI1000

40.506

100

0.408
  pilA Acinetobacter nosocomialis M2

52.066

77.07

0.401
  pilA Pseudomonas aeruginosa PAK

38.994

100

0.395
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.667

95.541

0.389
  pilA Acinetobacter baumannii strain A118

37.5

96.815

0.363