Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   P0083_RS10060 Genome accession   NZ_CP119528
Coordinates   1974727..1975002 (-) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain 3143     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1929543..1974703 1974727..1975002 flank 24


Gene organization within MGE regions


Location: 1929543..1975002
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P0083_RS09740 (P0083_09740) - 1929543..1930550 (-) 1008 WP_002357063.1 class I SAM-dependent methyltransferase -
  P0083_RS09745 (P0083_09745) comGG 1930679..1931032 (-) 354 WP_002357061.1 competence type IV pilus minor pilin ComGG -
  P0083_RS09750 (P0083_09750) - 1931090..1932262 (-) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  P0083_RS09755 (P0083_09755) comGF 1932364..1932798 (-) 435 WP_002357060.1 competence type IV pilus minor pilin ComGF -
  P0083_RS09760 (P0083_09760) - 1932788..1933159 (-) 372 WP_002365830.1 type II secretion system protein -
  P0083_RS09765 (P0083_09765) - 1933493..1933618 (+) 126 WP_002364184.1 hypothetical protein -
  P0083_RS09770 (P0083_09770) hemH 1933915..1934856 (-) 942 WP_002364185.1 ferrochelatase -
  P0083_RS09780 (P0083_09780) - 1935596..1935844 (-) 249 WP_002383136.1 hypothetical protein -
  P0083_RS09785 (P0083_09785) - 1935916..1936116 (-) 201 WP_002357053.1 cold-shock protein -
  P0083_RS09790 (P0083_09790) - 1936953..1938194 (-) 1242 WP_002370962.1 LysM peptidoglycan-binding domain-containing protein -
  P0083_RS09795 (P0083_09795) - 1938195..1938428 (-) 234 WP_002384371.1 phage holin -
  P0083_RS09800 (P0083_09800) - 1938421..1938642 (-) 222 WP_002364191.1 hypothetical protein -
  P0083_RS09805 (P0083_09805) - 1938677..1938832 (-) 156 WP_002364192.1 XkdX family protein -
  P0083_RS09810 (P0083_09810) - 1938834..1939154 (-) 321 WP_002389262.1 hypothetical protein -
  P0083_RS09815 (P0083_09815) - 1939168..1939659 (-) 492 WP_002364194.1 hypothetical protein -
  P0083_RS09820 (P0083_09820) - 1939659..1939946 (-) 288 WP_002357046.1 collagen-like protein -
  P0083_RS09825 (P0083_09825) - 1939943..1940539 (-) 597 WP_002357045.1 hypothetical protein -
  P0083_RS09830 (P0083_09830) - 1940532..1941413 (-) 882 WP_002387290.1 phage baseplate upper protein -
  P0083_RS09835 (P0083_09835) - 1941432..1944239 (-) 2808 WP_002387289.1 phage tail spike protein -
  P0083_RS09840 (P0083_09840) - 1944221..1944955 (-) 735 WP_002357042.1 hypothetical protein -
  P0083_RS09845 (P0083_09845) - 1944945..1947842 (-) 2898 WP_276131437.1 tape measure protein -
  P0083_RS09850 (P0083_09850) - 1948090..1948440 (-) 351 WP_002357039.1 hypothetical protein -
  P0083_RS09855 (P0083_09855) - 1948493..1949341 (-) 849 WP_002387287.1 major tail protein -
  P0083_RS09860 (P0083_09860) - 1949342..1949716 (-) 375 WP_002387286.1 DUF6838 family protein -
  P0083_RS09865 (P0083_09865) - 1949719..1950117 (-) 399 WP_002357036.1 HK97 gp10 family phage protein -
  P0083_RS09870 (P0083_09870) - 1950110..1950484 (-) 375 WP_002387285.1 hypothetical protein -
  P0083_RS09875 (P0083_09875) - 1950481..1950825 (-) 345 WP_002387282.1 hypothetical protein -
  P0083_RS09880 (P0083_09880) - 1950839..1951021 (-) 183 WP_002387281.1 hypothetical protein -
  P0083_RS09885 (P0083_09885) - 1951050..1951937 (-) 888 WP_002387280.1 DUF5309 domain-containing protein -
  P0083_RS09890 (P0083_09890) - 1951951..1952574 (-) 624 WP_010706493.1 DUF4355 domain-containing protein -
  P0083_RS09895 (P0083_09895) - 1952743..1953063 (-) 321 WP_002380436.1 hypothetical protein -
  P0083_RS09900 (P0083_09900) - 1953120..1953350 (-) 231 WP_002380437.1 hypothetical protein -
  P0083_RS09905 (P0083_09905) - 1953351..1955255 (-) 1905 WP_010706494.1 head protein -
  P0083_RS09910 (P0083_09910) - 1955230..1956702 (-) 1473 WP_010706495.1 phage portal protein -
  P0083_RS09915 (P0083_09915) terL 1956714..1958102 (-) 1389 WP_002387276.1 phage terminase large subunit -
  P0083_RS09920 (P0083_09920) - 1958095..1958556 (-) 462 WP_002383816.1 helix-turn-helix domain-containing protein -
  P0083_RS09925 (P0083_09925) - 1958632..1958808 (-) 177 WP_010706496.1 hypothetical protein -
  P0083_RS09930 (P0083_09930) - 1958992..1959804 (-) 813 WP_002387275.1 hypothetical protein -
  P0083_RS09935 (P0083_09935) - 1959916..1960149 (-) 234 Protein_1929 hypothetical protein -
  P0083_RS09940 (P0083_09940) - 1960733..1961203 (-) 471 WP_002383811.1 hypothetical protein -
  P0083_RS09945 (P0083_09945) - 1961228..1962151 (-) 924 WP_002383810.1 DUF6731 family protein -
  P0083_RS09955 (P0083_09955) - 1962869..1963285 (-) 417 WP_010706497.1 ArpU family phage packaging/lysis transcriptional regulator -
  P0083_RS09960 (P0083_09960) - 1964193..1964600 (-) 408 WP_025191675.1 RusA family crossover junction endodeoxyribonuclease -
  P0083_RS09965 (P0083_09965) - 1964597..1965112 (-) 516 Protein_1934 helix-turn-helix domain-containing protein -
  P0083_RS09970 (P0083_09970) - 1965214..1966386 (+) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  P0083_RS09975 (P0083_09975) - 1966439..1966786 (-) 348 Protein_1936 helix-turn-helix domain-containing protein -
  P0083_RS09980 (P0083_09980) - 1966786..1966986 (-) 201 WP_002357010.1 hypothetical protein -
  P0083_RS09985 (P0083_09985) - 1966991..1967374 (-) 384 WP_002383802.1 putative HNHc nuclease -
  P0083_RS09990 (P0083_09990) - 1967413..1967631 (-) 219 WP_002383801.1 hypothetical protein -
  P0083_RS09995 (P0083_09995) - 1967636..1968370 (-) 735 WP_002383799.1 ERF family protein -
  P0083_RS10000 (P0083_10000) - 1968363..1968680 (-) 318 WP_002383798.1 hypothetical protein -
  P0083_RS10005 (P0083_10005) - 1968876..1969214 (-) 339 WP_002383796.1 hypothetical protein -
  P0083_RS10010 (P0083_10010) - 1969251..1969460 (-) 210 WP_002378465.1 hypothetical protein -
  P0083_RS10015 (P0083_10015) - 1969515..1969703 (+) 189 WP_002357001.1 YegP family protein -
  P0083_RS10020 (P0083_10020) - 1969729..1970451 (-) 723 WP_002387270.1 phage regulatory protein -
  P0083_RS10025 (P0083_10025) - 1970474..1970785 (-) 312 WP_002403885.1 hypothetical protein -
  P0083_RS10030 (P0083_10030) - 1970797..1970988 (-) 192 WP_002383936.1 hypothetical protein -
  P0083_RS10035 (P0083_10035) - 1971284..1971625 (+) 342 WP_002387267.1 helix-turn-helix transcriptional regulator -
  P0083_RS10040 (P0083_10040) - 1971630..1972280 (+) 651 WP_002387266.1 ImmA/IrrE family metallo-endopeptidase -
  P0083_RS10045 (P0083_10045) - 1972376..1973005 (+) 630 WP_002387265.1 SHOCT domain-containing protein -
  P0083_RS10050 (P0083_10050) - 1973111..1974259 (+) 1149 WP_002387264.1 site-specific integrase -
  P0083_RS10055 (P0083_10055) comGD 1974296..1974730 (-) 435 Protein_1952 competence type IV pilus minor pilin ComGD -
  P0083_RS10060 (P0083_10060) comGC/cglC 1974727..1975002 (-) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=799527 P0083_RS10060 WP_002356991.1 1974727..1975002(-) (comGC/cglC) [Enterococcus faecalis strain 3143]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=799527 P0083_RS10060 WP_002356991.1 1974727..1975002(-) (comGC/cglC) [Enterococcus faecalis strain 3143]
ATGAAAAAGAAACAAAAATACGCAGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTCCCTAACTTAGCGAAACATAAAGAAACAGTTGATAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCCTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396