Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   P0M04_RS03775 Genome accession   NZ_CP119520
Coordinates   850239..851429 (-) Length   396 a.a.
NCBI ID   WP_281042373.1    Uniprot ID   -
Organism   Telluria mixta strain ACM 1762     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 845239..856429
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P0M04_RS03760 (P0M04_03770) - 845459..848263 (-) 2805 WP_259448691.1 type VI secretion system Vgr family protein -
  P0M04_RS03765 (P0M04_03775) - 849366..849476 (+) 111 Protein_746 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
  P0M04_RS03770 (P0M04_03780) rfaE2 849749..850228 (-) 480 WP_259448690.1 D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase -
  P0M04_RS03775 (P0M04_03785) pilU 850239..851429 (-) 1191 WP_281042373.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  P0M04_RS03780 (P0M04_03790) - 851572..852516 (+) 945 WP_259448689.1 hydrogen peroxide-inducible genes activator -
  P0M04_RS03785 (P0M04_03795) - 852494..853015 (-) 522 WP_259448688.1 hypothetical protein -
  P0M04_RS03790 (P0M04_03800) ubiA 853183..854037 (+) 855 WP_259448687.1 4-hydroxybenzoate octaprenyltransferase -
  P0M04_RS03795 (P0M04_03805) - 854076..854396 (+) 321 WP_036230048.1 YqjD family protein -
  P0M04_RS03800 (P0M04_03810) - 854396..854797 (+) 402 WP_259448686.1 phage holin family protein -
  P0M04_RS03805 (P0M04_03815) - 854784..855077 (+) 294 WP_259448685.1 hypothetical protein -
  P0M04_RS03810 (P0M04_03820) - 855079..855456 (+) 378 WP_259448684.1 hypothetical protein -
  P0M04_RS03815 (P0M04_03825) - 855465..856100 (-) 636 WP_259448683.1 hypothetical protein -

Sequence

Protein


Download         Length: 396 a.a.        Molecular weight: 43807.12 Da        Isoelectric Point: 6.2369

>NTDB_id=799414 P0M04_RS03775 WP_281042373.1 850239..851429(-) (pilU) [Telluria mixta strain ACM 1762]
MSSTFGPAEAQAYMHKLLTVMHQQGGSDLFISADFPPSMKHQGAMKPLSQQRLTGDITRALSLSLMNERQRAEFEAEMEC
NFAISLPGVCRFRVNVFVQQQSVSMVVRTIASEIPNFEKLDLPEVLKDVVMTKRGLVLVVGGTGSGKSTTLAAMIDYRNS
NSAGHIITVEDPVEYVHKNKSCLITHREVGVDTLSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQTMDRIINFFPEERRNQLLMDLSSNLRAIVSQRLVRTEDGKGRKAAIEILLNTPTIGEMILKGNFHSIKEIMHKS
RELGMCTFDQALFELYNKGFISYDEAIRNADSANGLRLQIKLSGERKAPDEGGGAAKPAELSMLVDEPEEEPPAAS

Nucleotide


Download         Length: 1191 bp        

>NTDB_id=799414 P0M04_RS03775 WP_281042373.1 850239..851429(-) (pilU) [Telluria mixta strain ACM 1762]
ATGTCCTCGACCTTCGGCCCGGCGGAAGCCCAGGCCTATATGCACAAGCTGCTCACGGTCATGCACCAGCAGGGCGGCTC
CGACCTGTTCATCTCGGCCGATTTCCCGCCCAGCATGAAGCACCAGGGCGCCATGAAACCCCTGAGCCAACAGCGCCTGA
CGGGCGACATTACGCGCGCGCTGTCGCTCTCGCTGATGAACGAGCGCCAGCGCGCCGAGTTCGAGGCCGAGATGGAGTGC
AATTTCGCGATCTCCCTGCCGGGCGTGTGCCGCTTCCGTGTGAACGTGTTCGTGCAACAGCAGAGTGTGAGCATGGTCGT
GCGAACCATTGCATCGGAAATCCCGAATTTCGAAAAGCTCGACCTGCCCGAGGTGCTGAAGGACGTCGTGATGACGAAGC
GCGGGCTCGTCCTCGTCGTCGGCGGTACCGGCTCCGGCAAGTCGACGACGCTGGCTGCGATGATCGATTACCGCAACAGC
AACTCGGCCGGCCACATCATCACGGTCGAGGACCCTGTCGAATACGTCCACAAGAACAAGAGCTGCCTCATCACGCACCG
CGAGGTAGGCGTCGACACGCTGTCGTGGCACAACGCGCTGAAGAACACGCTGCGCCAGGCACCGGACGTGATCCTGATCG
GTGAGATCCGCGACACGGAAACGATGGAACATGCGATCGCGTTCGCCGAAACAGGGCACTTATGCCTGGGCACACTGCAC
GCGAACAATGCGAACCAGACGATGGACCGCATCATCAACTTCTTCCCGGAAGAGCGGCGGAACCAGCTGTTGATGGACTT
GTCGTCGAACCTGCGCGCCATCGTCTCGCAGCGGCTGGTCCGCACGGAAGATGGGAAAGGGCGCAAGGCTGCCATCGAGA
TCTTGCTGAACACACCGACGATCGGCGAGATGATCCTGAAAGGCAATTTCCACAGTATCAAGGAGATCATGCACAAGTCG
CGCGAGCTGGGCATGTGCACGTTCGACCAGGCGTTGTTCGAGCTCTACAACAAGGGCTTCATCAGCTACGACGAAGCCAT
CCGCAACGCCGATTCCGCGAACGGCCTGCGTCTGCAGATCAAGCTGTCGGGAGAACGCAAGGCGCCGGACGAGGGCGGCG
GTGCTGCGAAGCCGGCCGAGCTGTCGATGCTGGTGGACGAGCCGGAAGAGGAACCGCCGGCGGCAAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(100-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.714

88.384

0.528
  pilU Acinetobacter baylyi ADP1

52.291

93.687

0.49
  pilU Vibrio cholerae strain A1552

48.267

94.697

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.713

84.343

0.369
  pilT Pseudomonas aeruginosa PAK

43.582

84.596

0.369
  pilT Legionella pneumophila strain Lp02

44.648

82.576

0.369
  pilT Legionella pneumophila strain ERS1305867

44.648

82.576

0.369
  pilT Pseudomonas stutzeri DSM 10701

42.985

84.596

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.333

83.333

0.361
  pilT Vibrio cholerae strain A1552

43.333

83.333

0.361
  pilT Acinetobacter baylyi ADP1

43.202

83.586

0.361