Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   RBB89_RS25790 Genome accession   NZ_CP132949
Coordinates   5520922..5521395 (-) Length   157 a.a.
NCBI ID   WP_015503553.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain IPA34     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5515922..5526395
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RBB89_RS25770 (RBB89_25775) ampD 5516663..5517229 (-) 567 WP_009875882.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RBB89_RS25775 (RBB89_25780) - 5517380..5519635 (-) 2256 WP_019726810.1 DUF1631 domain-containing protein -
  RBB89_RS25780 (RBB89_25785) nadC 5519921..5520769 (+) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RBB89_RS25790 (RBB89_25795) pilE 5520922..5521395 (-) 474 WP_015503553.1 pilin Machinery gene
  RBB89_RS25795 (RBB89_25800) pilB 5521626..5523326 (+) 1701 WP_003120945.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RBB89_RS25800 (RBB89_25805) pilC 5523330..5524547 (+) 1218 WP_010792214.1 type II secretion system F family protein Machinery gene
  RBB89_RS25805 (RBB89_25810) pilD 5524550..5525422 (+) 873 WP_019726811.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  RBB89_RS25810 (RBB89_25815) coaE 5525419..5526030 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  RBB89_RS25815 (RBB89_25820) yacG 5526027..5526227 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 16090.45 Da        Isoelectric Point: 8.4684

>NTDB_id=797422 RBB89_RS25790 WP_015503553.1 5520922..5521395(-) (pilE) [Pseudomonas aeruginosa strain IPA34]
MKAAQKGFTLIELMIVVAIIGILAAIAIPAYQDYTARAQLSEAMTLASGLKTKVSDIFSQDGSCPANTAATAGIEKDTDI
NGKYVAKVTTGGTAAASGGCTIVATMKASDVATPLRGKTLTLTLGNADKGSYTWACTSNADNKYLPKTCQTATTTTP

Nucleotide


Download         Length: 474 bp        

>NTDB_id=797422 RBB89_RS25790 WP_015503553.1 5520922..5521395(-) (pilE) [Pseudomonas aeruginosa strain IPA34]
ATGAAAGCTGCTCAAAAAGGCTTTACCTTGATCGAATTGATGATCGTGGTCGCGATCATCGGTATTCTGGCTGCCATCGC
TATTCCAGCGTATCAGGACTACACCGCACGCGCTCAGCTTAGCGAAGCCATGACCCTGGCCAGTGGTCTCAAGACGAAAG
TGAGCGATATCTTCTCTCAGGATGGGTCCTGCCCGGCTAATACTGCTGCCACGGCAGGCATCGAGAAAGATACCGACATC
AACGGCAAGTATGTTGCCAAGGTAACAACTGGTGGCACCGCAGCTGCGTCTGGTGGTTGCACTATCGTTGCTACTATGAA
AGCCTCTGATGTGGCTACTCCTCTGAGGGGGAAAACTCTGACTTTGACTCTAGGAAATGCTGACAAGGGTTCTTACACTT
GGGCCTGTACTTCCAACGCAGATAACAAGTACCTGCCAAAAACCTGCCAGACTGCTACCACTACCACTCCGTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(36-150)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

42.197

100

0.465
  comP Acinetobacter baylyi ADP1

47.059

97.452

0.459
  pilE Neisseria gonorrhoeae MS11

42.515

100

0.452
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

41.176

100

0.446
  pilA/pilA1 Eikenella corrodens VA1

41.975

100

0.433
  pilA2 Legionella pneumophila str. Paris

45.578

93.631

0.427
  pilA2 Legionella pneumophila strain ERS1305867

44.218

93.631

0.414
  pilA Ralstonia pseudosolanacearum GMI1000

39.394

100

0.414
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.333

95.541

0.414
  pilA Pseudomonas aeruginosa PAK

41.722

96.178

0.401
  pilA Acinetobacter nosocomialis M2

55.357

71.338

0.395