Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   PYW30_RS00670 Genome accession   NZ_CP118950
Coordinates   137170..138114 (+) Length   314 a.a.
NCBI ID   WP_042218031.1    Uniprot ID   -
Organism   Lactococcus garvieae subsp. garvieae strain DSM 20684     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 132170..143114
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYW30_RS00655 - 133570..135024 (-) 1455 WP_042218033.1 GH25 family lysozyme -
  PYW30_RS00660 guaB 135193..136674 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  PYW30_RS00665 rpsU 136797..136973 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  PYW30_RS00670 comGA 137170..138114 (+) 945 WP_042218031.1 competence type IV pilus ATPase ComGA Machinery gene
  PYW30_RS00675 comGB 138053..139072 (+) 1020 WP_255204098.1 competence type IV pilus assembly protein ComGB Machinery gene
  PYW30_RS00680 comYC 139091..139396 (+) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  PYW30_RS00685 comGD 139371..139745 (+) 375 WP_042218026.1 competence type IV pilus minor pilin ComGD -
  PYW30_RS10620 comGE 139648..140022 (+) 375 Protein_127 competence type IV pilus minor pilin ComGE -
  PYW30_RS10625 comYF 140032..140472 (+) 441 WP_042218182.1 competence type IV pilus minor pilin ComGF Machinery gene
  PYW30_RS00695 - 140705..141550 (+) 846 WP_042218024.1 zinc ABC transporter substrate-binding protein -
  PYW30_RS00700 - 141636..142505 (-) 870 WP_042218022.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35889.17 Da        Isoelectric Point: 6.9763

>NTDB_id=796664 PYW30_RS00670 WP_042218031.1 137170..138114(+) (comGA) [Lactococcus garvieae subsp. garvieae strain DSM 20684]
MIQVLAKKLLQKAVDQMVHDLYFVALEGKYSLYFRTATERRFERELDFEQGQALIAHMKFLSGMNLGESRRVQLGACTYV
LDKGEQRLRLSTVGDFHGQESLVIRLLHHQQSQLHFWNSEIFKSFIGGRGLYLFSGPVGSGKTSLMYKFAREHFKNQQVI
CIEDPVELVETEFLQLQVNKVIGNDYDALIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVIAR
LKELGLTDWELQSSLQRVIYQRLIAGKGLLVYEKEKFEEWQPDEWNGQIDQLVADGFISAVTAAREKIEFSEAD

Nucleotide


Download         Length: 945 bp        

>NTDB_id=796664 PYW30_RS00670 WP_042218031.1 137170..138114(+) (comGA) [Lactococcus garvieae subsp. garvieae strain DSM 20684]
ATGATACAGGTTTTGGCAAAAAAACTTTTACAAAAAGCAGTAGATCAGATGGTGCATGATCTTTATTTCGTAGCATTAGA
AGGAAAGTATTCTCTCTATTTTCGTACAGCAACAGAAAGGAGGTTTGAAAGAGAACTCGATTTTGAACAAGGGCAAGCAC
TGATTGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGGCGGGTACAGTTGGGGGCTTGCACTTATGTT
TTAGATAAAGGTGAGCAGCGTTTACGTTTATCTACAGTCGGTGATTTTCATGGTCAAGAAAGCTTGGTTATCCGACTTCT
GCATCATCAACAAAGCCAACTTCATTTTTGGAATTCAGAGATTTTTAAATCTTTTATTGGTGGGAGAGGGCTTTACCTGT
TTTCCGGTCCCGTAGGCTCAGGTAAGACCAGCCTCATGTACAAGTTTGCAAGGGAGCATTTTAAAAATCAACAAGTTATT
TGTATAGAAGATCCCGTGGAGTTAGTGGAAACTGAGTTTTTACAACTTCAAGTCAATAAAGTAATAGGGAATGATTACGA
TGCTTTGATTAAGCTCTCTCTTCGCCACAGGCCTGATTTACTCATTGTTGGTGAAATTAGGGACAGTCAGACGGCAAAAG
CTGTTCTCCGCGCAAGTTTGACGGGATATACAGTGTTTTCTACTGTTCATGCGCGCTCAATATCGGGTGTAATTGCACGG
CTGAAGGAACTGGGGTTAACTGATTGGGAGCTACAATCCAGCCTTCAGCGGGTGATTTATCAGCGCTTAATTGCAGGAAA
GGGGTTGCTTGTTTATGAAAAAGAAAAGTTTGAAGAATGGCAACCAGATGAATGGAACGGCCAGATTGATCAGTTGGTTG
CAGATGGATTTATCTCAGCTGTTACAGCTGCGCGCGAAAAAATTGAGTTTAGCGAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

59.873

100

0.599
  comYA Streptococcus gordonii str. Challis substr. CH1

53.968

100

0.541
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.674

99.682

0.535
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

53.355

99.682

0.532
  comGA/cglA/cilD Streptococcus pneumoniae D39

53.355

99.682

0.532
  comGA/cglA/cilD Streptococcus pneumoniae R6

53.355

99.682

0.532
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

53.355

99.682

0.532
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

52.396

99.682

0.522
  comYA Streptococcus mutans UA159

51.923

99.363

0.516
  comYA Streptococcus mutans UA140

51.923

99.363

0.516