Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   PYW30_RS10625 Genome accession   NZ_CP118950
Coordinates   140032..140472 (+) Length   146 a.a.
NCBI ID   WP_042218182.1    Uniprot ID   -
Organism   Lactococcus garvieae subsp. garvieae strain DSM 20684     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 135032..145472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYW30_RS00660 guaB 135193..136674 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  PYW30_RS00665 rpsU 136797..136973 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  PYW30_RS00670 comGA 137170..138114 (+) 945 WP_042218031.1 competence type IV pilus ATPase ComGA Machinery gene
  PYW30_RS00675 comGB 138053..139072 (+) 1020 WP_255204098.1 competence type IV pilus assembly protein ComGB Machinery gene
  PYW30_RS00680 comYC 139091..139396 (+) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  PYW30_RS00685 comGD 139371..139745 (+) 375 WP_042218026.1 competence type IV pilus minor pilin ComGD -
  PYW30_RS10620 comGE 139648..140022 (+) 375 Protein_127 competence type IV pilus minor pilin ComGE -
  PYW30_RS10625 comYF 140032..140472 (+) 441 WP_042218182.1 competence type IV pilus minor pilin ComGF Machinery gene
  PYW30_RS00695 - 140705..141550 (+) 846 WP_042218024.1 zinc ABC transporter substrate-binding protein -
  PYW30_RS00700 - 141636..142505 (-) 870 WP_042218022.1 RluA family pseudouridine synthase -
  PYW30_RS00705 - 142581..144869 (+) 2289 WP_042218020.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 16788.34 Da        Isoelectric Point: 8.8685

>NTDB_id=796705 PYW30_RS10625 WP_042218182.1 140032..140472(+) (comYF) [Lactococcus garvieae subsp. garvieae strain DSM 20684]
MVLEKNKVTAFTLLESLLALLVLVGTFSLFLGMTKMFHEEVKRATTDHTQDWQLFCSLLRSELEGASLDKVENNYLYVRK
HVNLRFGLSSQGDFRKTNANGRGYQPMIHHLKNAKISQEGEQIKIILTFEKGGDRTFLYTFPEKES

Nucleotide


Download         Length: 441 bp        

>NTDB_id=796705 PYW30_RS10625 WP_042218182.1 140032..140472(+) (comYF) [Lactococcus garvieae subsp. garvieae strain DSM 20684]
ATTGTTCTTGAAAAAAATAAAGTTACAGCCTTTACACTTTTGGAATCACTCCTTGCTTTACTCGTGTTAGTGGGCACTTT
TTCTCTTTTTCTAGGTATGACTAAAATGTTTCATGAGGAAGTGAAACGTGCTACAACAGACCATACCCAAGACTGGCAAC
TTTTTTGTAGTCTACTGAGAAGTGAACTTGAGGGAGCAAGTTTAGATAAGGTAGAAAATAACTATCTTTATGTACGCAAA
CATGTTAACCTAAGGTTTGGCCTTTCCTCTCAAGGGGATTTTCGAAAAACTAACGCGAATGGACGAGGCTATCAGCCCAT
GATTCATCATTTAAAAAATGCAAAAATCAGCCAAGAGGGAGAGCAGATAAAAATTATCTTGACTTTTGAAAAAGGAGGAG
ACCGCACATTTCTTTACACGTTCCCAGAGAAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(40-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

50.355

96.575

0.486
  comYF Streptococcus mutans UA159

49.645

96.575

0.479
  comGF Lactococcus lactis subsp. cremoris KW2

49.64

95.205

0.473
  comGF/cglF Streptococcus pneumoniae Rx1

40.845

97.26

0.397
  comGF/cglF Streptococcus pneumoniae R6

40.845

97.26

0.397
  comGF/cglF Streptococcus pneumoniae TIGR4

40.845

97.26

0.397
  comGF/cglF Streptococcus pneumoniae D39

40.845

97.26

0.397
  comGF/cglF Streptococcus mitis NCTC 12261

38.732

97.26

0.377
  comGF/cglF Streptococcus mitis SK321

38.028

97.26

0.37