Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   PYW30_RS00675 Genome accession   NZ_CP118950
Coordinates   138053..139072 (+) Length   339 a.a.
NCBI ID   WP_255204098.1    Uniprot ID   -
Organism   Lactococcus garvieae subsp. garvieae strain DSM 20684     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 133053..144072
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYW30_RS00655 - 133570..135024 (-) 1455 WP_042218033.1 GH25 family lysozyme -
  PYW30_RS00660 guaB 135193..136674 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  PYW30_RS00665 rpsU 136797..136973 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  PYW30_RS00670 comGA 137170..138114 (+) 945 WP_042218031.1 competence type IV pilus ATPase ComGA Machinery gene
  PYW30_RS00675 comGB 138053..139072 (+) 1020 WP_255204098.1 competence type IV pilus assembly protein ComGB Machinery gene
  PYW30_RS00680 comYC 139091..139396 (+) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  PYW30_RS00685 comGD 139371..139745 (+) 375 WP_042218026.1 competence type IV pilus minor pilin ComGD -
  PYW30_RS10620 comGE 139648..140022 (+) 375 Protein_127 competence type IV pilus minor pilin ComGE -
  PYW30_RS10625 comYF 140032..140472 (+) 441 WP_042218182.1 competence type IV pilus minor pilin ComGF Machinery gene
  PYW30_RS00695 - 140705..141550 (+) 846 WP_042218024.1 zinc ABC transporter substrate-binding protein -
  PYW30_RS00700 - 141636..142505 (-) 870 WP_042218022.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 39361.05 Da        Isoelectric Point: 9.7389

>NTDB_id=796665 PYW30_RS00675 WP_255204098.1 138053..139072(+) (comGB) [Lactococcus garvieae subsp. garvieae strain DSM 20684]
MDLSQLLQLRAKKLSLAKQIKLIQLMNNLFKSGFHLSEIIDFLERSGLTEAYFISKMREGLLNGQSFSGILAKLKFNEDI
VTQVSLAESHGNVEYTLGLIEEKLRRVLNIRKKLIQVATYPLVLLAFLIFIMLGLKNYLLPQLDENRGLATYVIQNLPTL
FLGCLFSLLVFFYLGRYYWKKKTALEAMRLMVKIPFVGRFVQLYLTAYFSREWGNLIAQAVDLRQICFIMQEQKSRIFKE
FGFELLQILDRGQKFEDALRLYPIFTKELALIVEYGELKSKLGKELMVFSEESWSLFFERVERAMQLIQPIVFLLVALLI
ILIYAAMLLPIYGNMGNLI

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=796665 PYW30_RS00675 WP_255204098.1 138053..139072(+) (comGB) [Lactococcus garvieae subsp. garvieae strain DSM 20684]
ATGGATTTATCTCAGCTGTTACAGCTGCGCGCGAAAAAATTGAGTTTAGCGAAGCAGATTAAGCTTATTCAACTTATGAA
TAATCTTTTCAAAAGTGGCTTTCACCTTTCTGAAATTATTGATTTTCTTGAAAGGTCGGGTCTCACGGAAGCATACTTTA
TTTCCAAAATGCGTGAAGGTTTACTCAACGGGCAAAGTTTCTCTGGTATTTTAGCGAAGCTCAAATTTAATGAGGATATT
GTGACTCAAGTCTCTTTGGCCGAGTCTCACGGGAATGTTGAGTATACTTTAGGGTTGATTGAAGAAAAACTGAGACGCGT
TTTGAACATTCGAAAAAAGCTCATCCAAGTTGCCACATACCCGCTCGTTCTTCTGGCATTTCTAATCTTTATCATGCTAG
GGTTGAAAAATTATTTACTGCCTCAACTGGACGAAAACAGAGGATTAGCAACTTATGTTATACAGAATCTTCCGACCCTC
TTTTTAGGGTGCCTCTTCAGTTTGCTTGTTTTCTTTTATCTGGGCAGATACTATTGGAAGAAAAAAACTGCTTTAGAAGC
GATGAGGCTAATGGTCAAAATACCTTTTGTTGGCCGTTTTGTCCAGCTTTATTTGACAGCTTATTTTTCAAGAGAATGGG
GAAACTTAATTGCCCAAGCAGTTGATTTACGTCAGATTTGCTTTATTATGCAAGAACAAAAAAGTCGAATCTTCAAAGAA
TTTGGTTTTGAGCTCCTTCAAATATTAGATAGGGGTCAGAAGTTTGAAGATGCCCTTCGACTTTACCCCATATTTACCAA
GGAGTTGGCATTAATTGTGGAATATGGTGAACTAAAGTCTAAGCTGGGGAAAGAGTTAATGGTTTTTTCTGAAGAAAGCT
GGTCGCTCTTTTTTGAAAGAGTTGAAAGAGCAATGCAACTTATCCAGCCTATCGTTTTCTTGCTCGTGGCGCTGTTGATT
ATTTTAATTTATGCTGCAATGTTACTTCCTATATATGGCAATATGGGAAACTTGATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

53.55

99.705

0.534
  comGB/cglB Streptococcus mitis NCTC 12261

52.836

98.82

0.522
  comGB/cglB Streptococcus pneumoniae D39

52.537

98.82

0.519
  comGB/cglB Streptococcus pneumoniae R6

52.537

98.82

0.519
  comGB/cglB Streptococcus pneumoniae TIGR4

52.537

98.82

0.519
  comGB/cglB Streptococcus mitis SK321

52.537

98.82

0.519
  comGB/cglB Streptococcus pneumoniae Rx1

52.537

98.82

0.519
  comYB Streptococcus gordonii str. Challis substr. CH1

52.083

99.115

0.516
  comYB Streptococcus mutans UA140

51.266

93.215

0.478
  comYB Streptococcus mutans UA159

50.949

93.215

0.475