Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   PWO50_RS02870 Genome accession   NZ_CP118914
Coordinates   642432..642896 (+) Length   154 a.a.
NCBI ID   WP_210797577.1    Uniprot ID   -
Organism   Pseudoalteromonas ostreae strain hOe-125     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 637432..647896
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWO50_RS02850 pdhR 638137..638886 (-) 750 WP_210797581.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  PWO50_RS02855 ampE 639234..640085 (-) 852 WP_210797580.1 beta-lactamase regulator AmpE -
  PWO50_RS02860 ampD 640134..640676 (-) 543 WP_210797579.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PWO50_RS02865 nadC 641058..641903 (+) 846 WP_210797578.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PWO50_RS02870 pilE 642432..642896 (+) 465 WP_210797577.1 pilin Machinery gene
  PWO50_RS02875 - 643225..644268 (+) 1044 WP_228732993.1 O-antigen ligase family protein -
  PWO50_RS02880 pilC 644271..645500 (+) 1230 WP_210797575.1 type II secretion system F family protein Machinery gene
  PWO50_RS02885 pilD 646095..646994 (+) 900 WP_210797574.1 A24 family peptidase Machinery gene
  PWO50_RS02890 coaE 646996..647631 (+) 636 WP_210797573.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 15841.05 Da        Isoelectric Point: 6.2043

>NTDB_id=796285 PWO50_RS02870 WP_210797577.1 642432..642896(+) (pilE) [Pseudoalteromonas ostreae strain hOe-125]
MEKMTQQNQKGFTLIELMIVVAIIGILAAVALPAYQDYTTRAKMSEVVTFAGSAKTAVSECAITTGDTTLAACNSNSKVG
LDPTATNISSTYVESVTVGTGGVVTAAIQGTSVTALDGGSLTFTPTATSGSVSWKCEISAATLNKYVPQNCRKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=796285 PWO50_RS02870 WP_210797577.1 642432..642896(+) (pilE) [Pseudoalteromonas ostreae strain hOe-125]
ATGGAAAAAATGACACAACAAAATCAAAAGGGTTTTACCCTAATTGAATTAATGATTGTAGTAGCAATCATCGGTATTTT
GGCAGCAGTCGCGTTACCTGCCTATCAAGATTATACTACTCGTGCGAAAATGAGTGAAGTTGTTACTTTTGCTGGCTCAG
CTAAAACAGCAGTTAGTGAATGCGCAATCACAACAGGAGACACAACACTTGCCGCATGTAACAGTAATTCAAAAGTAGGA
TTGGATCCTACTGCAACTAACATATCTTCTACTTATGTAGAATCAGTAACAGTTGGAACTGGTGGTGTCGTTACTGCAGC
AATCCAGGGTACCTCTGTTACGGCACTTGATGGTGGTTCACTTACATTCACGCCTACAGCAACTAGTGGAAGTGTCAGCT
GGAAGTGCGAAATAAGCGCAGCAACGTTAAACAAATATGTGCCACAAAACTGCCGTAAAAGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-32)

Pilin (bacterial filament) (Pilin)

(40-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

40.217

100

0.481
  pilA2 Legionella pneumophila strain ERS1305867

49.315

94.805

0.468
  pilA2 Legionella pneumophila str. Paris

49.315

94.805

0.468
  pilA Ralstonia pseudosolanacearum GMI1000

39.106

100

0.455
  comP Acinetobacter baylyi ADP1

44.371

98.052

0.435
  pilE Neisseria gonorrhoeae strain FA1090

40.625

100

0.422
  pilA/pilA1 Eikenella corrodens VA1

38.994

100

0.403
  pilE Neisseria gonorrhoeae MS11

46.512

83.766

0.39
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.667

97.403

0.377