Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   PWP91_RS09375 Genome accession   NZ_CP118738
Coordinates   1891017..1891442 (-) Length   141 a.a.
NCBI ID   WP_195229129.1    Uniprot ID   -
Organism   Lactococcus lactis strain P-1     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1879140..1900464 1891017..1891442 within 0


Gene organization within MGE regions


Location: 1879140..1900464
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWP91_RS09280 (PWP91_09280) - 1879140..1879670 (-) 531 WP_075070535.1 hypothetical protein -
  PWP91_RS09285 (PWP91_09285) - 1879753..1880532 (-) 780 WP_251899735.1 peptidoglycan amidohydrolase family protein -
  PWP91_RS09290 (PWP91_09290) - 1880532..1880807 (-) 276 WP_139917173.1 holin -
  PWP91_RS09295 (PWP91_09295) - 1880820..1881041 (-) 222 WP_012897995.1 hemolysin XhlA family protein -
  PWP91_RS09300 (PWP91_09300) - 1881062..1882156 (-) 1095 WP_264653715.1 pyocin knob domain-containing protein -
  PWP91_RS09305 (PWP91_09305) - 1882156..1882293 (-) 138 WP_195229120.1 hypothetical protein -
  PWP91_RS09310 (PWP91_09310) - 1882484..1883002 (-) 519 Protein_1794 phage tail protein -
  PWP91_RS09315 (PWP91_09315) - 1883001..1883591 (-) 591 Protein_1795 phage portal protein -
  PWP91_RS09320 (PWP91_09320) - 1883601..1884883 (-) 1283 Protein_1796 PBSX family phage terminase large subunit -
  PWP91_RS09325 (PWP91_09325) - 1884876..1885328 (-) 453 WP_139917177.1 terminase small subunit -
  PWP91_RS09330 (PWP91_09330) - 1885461..1886651 (-) 1191 WP_260317767.1 glycoside hydrolase family protein -
  PWP91_RS09335 (PWP91_09335) - 1886928..1887317 (-) 390 WP_139917180.1 DUF722 domain-containing protein -
  PWP91_RS09340 (PWP91_09340) - 1887434..1887703 (+) 270 WP_161935088.1 DUF3892 domain-containing protein -
  PWP91_RS09345 (PWP91_09345) - 1887824..1887979 (+) 156 WP_014572174.1 hypothetical protein -
  PWP91_RS09350 (PWP91_09350) - 1888199..1888585 (+) 387 WP_195229125.1 DUF805 domain-containing protein -
  PWP91_RS09355 (PWP91_09355) - 1888783..1889451 (+) 669 WP_139917187.1 type 1 glutamine amidotransferase -
  PWP91_RS09360 (PWP91_09360) - 1889553..1889774 (-) 222 WP_139917189.1 hypothetical protein -
  PWP91_RS09365 (PWP91_09365) - 1889755..1890558 (-) 804 WP_139917191.1 helix-turn-helix domain-containing protein -
  PWP91_RS09370 (PWP91_09370) - 1890558..1890884 (-) 327 WP_195229127.1 HNH endonuclease -
  PWP91_RS09375 (PWP91_09375) ssb 1891017..1891442 (-) 426 WP_195229129.1 single-stranded DNA-binding protein Machinery gene
  PWP91_RS13030 - 1891435..1892193 (-) 759 Protein_1808 Rad52/Rad22 family DNA repair protein -
  PWP91_RS09390 (PWP91_09390) - 1892202..1892597 (-) 396 WP_195229133.1 hypothetical protein -
  PWP91_RS09395 (PWP91_09395) - 1892698..1892934 (-) 237 WP_098384009.1 DUF1408 domain-containing protein -
  PWP91_RS09400 (PWP91_09400) - 1893030..1893254 (+) 225 WP_098384010.1 hypothetical protein -
  PWP91_RS09405 (PWP91_09405) - 1893485..1893823 (-) 339 WP_098397254.1 hypothetical protein -
  PWP91_RS09410 (PWP91_09410) - 1893981..1894631 (-) 651 WP_338084804.1 phage antirepressor -
  PWP91_RS09415 (PWP91_09415) - 1894647..1894895 (-) 249 WP_259771641.1 helix-turn-helix domain-containing protein -
  PWP91_RS09420 (PWP91_09420) - 1895031..1895369 (+) 339 Protein_1815 DUF2513 domain-containing protein -
  PWP91_RS09425 (PWP91_09425) - 1895340..1895612 (-) 273 WP_179884439.1 hypothetical protein -
  PWP91_RS09430 (PWP91_09430) - 1896078..1896221 (-) 144 WP_195229142.1 hypothetical protein -
  PWP91_RS09435 (PWP91_09435) - 1896476..1897000 (+) 525 WP_274998389.1 XRE family transcriptional regulator -
  PWP91_RS09440 (PWP91_09440) - 1897011..1897598 (+) 588 WP_274998390.1 hypothetical protein -
  PWP91_RS09445 (PWP91_09445) - 1897634..1897915 (+) 282 WP_260317773.1 hypothetical protein -
  PWP91_RS09450 (PWP91_09450) - 1897972..1898310 (+) 339 WP_195229148.1 DUF898 family protein -
  PWP91_RS09455 (PWP91_09455) - 1898413..1899189 (+) 777 WP_195229150.1 glycoside hydrolase, family 25 -
  PWP91_RS09460 (PWP91_09460) - 1899310..1900464 (+) 1155 WP_260317774.1 tyrosine-type recombinase/integrase -

Sequence


Protein


Download         Length: 141 a.a.        Molecular weight: 15824.59 Da        Isoelectric Point: 5.1972

>NTDB_id=794810 PWP91_RS09375 WP_195229129.1 1891017..1891442(-) (ssb) [Lactococcus lactis strain P-1]
MINNVTLVGRITKEPELRYTQQNKAVASFTLAVNRQFKNFNGEREADFINCVIWGKSAENLANWTHKGQLIGVTGSIQTR
NYENQQGQRVYIMEVVASNFQVLEKSNQANGERVGNPAAKPQNNDSFGSDPMEISDDDLPF

Nucleotide


Download         Length: 426 bp        

>NTDB_id=794810 PWP91_RS09375 WP_195229129.1 1891017..1891442(-) (ssb) [Lactococcus lactis strain P-1]
ATGATTAACAATGTCACTCTAGTAGGGCGAATCACTAAGGAACCTGAACTTAGATATACACAACAAAATAAAGCAGTTGC
TTCATTTACTCTTGCAGTTAATCGTCAATTTAAGAATTTCAATGGAGAAAGAGAAGCTGACTTTATCAATTGTGTTATTT
GGGGTAAATCAGCCGAAAACTTGGCCAATTGGACTCATAAAGGTCAATTAATTGGAGTTACTGGTAGTATTCAAACTCGC
AACTATGAGAATCAACAAGGGCAACGAGTTTATATTATGGAGGTTGTCGCAAGTAATTTCCAAGTACTTGAAAAAAGTAA
TCAAGCAAATGGTGAACGAGTTGGTAATCCAGCTGCAAAACCACAAAATAATGATTCTTTTGGAAGTGATCCAATGGAAA
TTTCAGATGATGACCTACCATTTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

53.529

100

0.645

  ssbA Bacillus subtilis subsp. subtilis str. 168

50.581

100

0.617

  ssbB/cilA Streptococcus mitis SK321

43.262

100

0.433

  ssbB/cilA Streptococcus mitis NCTC 12261

43.262

100

0.433

  ssbB/cilA Streptococcus pneumoniae TIGR4

43.262

100

0.433

  ssbB/cilA Streptococcus pneumoniae D39

42.553

100

0.426

  ssbB/cilA Streptococcus pneumoniae Rx1

42.553

100

0.426

  ssbB/cilA Streptococcus pneumoniae R6

42.553

100

0.426

  ssbB Streptococcus sobrinus strain NIDR 6715-7

42.553

100

0.426

  ssbB Bacillus subtilis subsp. subtilis str. 168

55.769

73.759

0.411

  ssbA Streptococcus mutans UA159

38.298

100

0.383

  ssbB Lactococcus lactis subsp. cremoris KW2

49.515

73.05

0.362