Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PUN47_RS12435 Genome accession   NZ_CP118599
Coordinates   2673244..2673675 (+) Length   143 a.a.
NCBI ID   WP_154184015.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 10M-VF     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2668244..2678675
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN47_RS12410 (PUN47_12410) dsbC 2669066..2669830 (-) 765 WP_020430455.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  PUN47_RS12415 (PUN47_12415) xerD 2669847..2670755 (-) 909 WP_020331983.1 site-specific tyrosine recombinase XerD -
  PUN47_RS12420 (PUN47_12420) fldB 2670896..2671417 (+) 522 WP_020430452.1 flavodoxin FldB -
  PUN47_RS12425 (PUN47_12425) ampD 2671422..2671973 (-) 552 WP_032081227.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PUN47_RS12430 (PUN47_12430) nadC 2672126..2673013 (+) 888 WP_020430449.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PUN47_RS12435 (PUN47_12435) pilA 2673244..2673675 (+) 432 WP_154184015.1 pilin Machinery gene
  PUN47_RS12440 (PUN47_12440) pilB 2673682..2675370 (+) 1689 WP_274888545.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PUN47_RS12445 (PUN47_12445) pilC 2675406..2676632 (+) 1227 WP_104966495.1 type II secretion system F family protein Machinery gene
  PUN47_RS12450 (PUN47_12450) pilD 2676684..2677553 (+) 870 WP_044366750.1 prepilin peptidase Machinery gene
  PUN47_RS12455 (PUN47_12455) coaE 2677556..2678164 (+) 609 WP_104966494.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14768.80 Da        Isoelectric Point: 8.4687

>NTDB_id=793854 PUN47_RS12435 WP_154184015.1 2673244..2673675(+) (pilA) [Vibrio fluvialis strain 10M-VF]
MNANQRKQQQGFTLIELMIVVAIIGVLSAIAVPAYKNYVAKSEAASALATLKSLITPAELYIQEKGTFKTTDQATLFSDV
GISAGSNSLGSLSVSADNTIKFEFTSGSLAASGSVGTITYTKTSTGWVCTTANLSTAKPSGCS

Nucleotide


Download         Length: 432 bp        

>NTDB_id=793854 PUN47_RS12435 WP_154184015.1 2673244..2673675(+) (pilA) [Vibrio fluvialis strain 10M-VF]
ATGAACGCGAATCAACGCAAACAACAGCAGGGCTTTACCCTGATCGAACTGATGATCGTGGTCGCCATCATCGGGGTTCT
GTCGGCGATTGCCGTACCCGCTTATAAAAACTATGTAGCCAAAAGTGAAGCGGCATCAGCGTTAGCAACTTTGAAGTCAC
TAATCACTCCAGCAGAGTTGTACATTCAAGAAAAAGGAACATTTAAGACAACTGACCAAGCTACGCTATTTTCAGATGTT
GGTATCTCTGCTGGCTCAAACAGTTTGGGTAGTTTGAGTGTTTCAGCTGATAACACCATTAAATTTGAGTTCACTTCTGG
ATCATTAGCAGCAAGTGGTTCAGTTGGAACAATAACTTATACAAAAACCTCAACTGGCTGGGTATGTACCACTGCAAACT
TAAGTACAGCTAAGCCAAGTGGCTGCTCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-31)

Pilin (bacterial filament) (Pilin)

(40-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

46

100

0.483
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.241

100

0.434
  pilA Vibrio cholerae strain A1552

39.241

100

0.434
  pilA Vibrio cholerae C6706

39.241

100

0.434
  pilA Pseudomonas aeruginosa PAK

41.216

100

0.427
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.621

100

0.392
  pilA Acinetobacter baumannii strain A118

36.601

100

0.392
  pilA/pilAI Pseudomonas stutzeri DSM 10701

34.868

100

0.371