Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PUN47_RS12445 Genome accession   NZ_CP118599
Coordinates   2675406..2676632 (+) Length   408 a.a.
NCBI ID   WP_104966495.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 10M-VF     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2670406..2681632
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN47_RS12420 (PUN47_12420) fldB 2670896..2671417 (+) 522 WP_020430452.1 flavodoxin FldB -
  PUN47_RS12425 (PUN47_12425) ampD 2671422..2671973 (-) 552 WP_032081227.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PUN47_RS12430 (PUN47_12430) nadC 2672126..2673013 (+) 888 WP_020430449.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PUN47_RS12435 (PUN47_12435) pilA 2673244..2673675 (+) 432 WP_154184015.1 pilin Machinery gene
  PUN47_RS12440 (PUN47_12440) pilB 2673682..2675370 (+) 1689 WP_274888545.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PUN47_RS12445 (PUN47_12445) pilC 2675406..2676632 (+) 1227 WP_104966495.1 type II secretion system F family protein Machinery gene
  PUN47_RS12450 (PUN47_12450) pilD 2676684..2677553 (+) 870 WP_044366750.1 prepilin peptidase Machinery gene
  PUN47_RS12455 (PUN47_12455) coaE 2677556..2678164 (+) 609 WP_104966494.1 dephospho-CoA kinase -
  PUN47_RS12460 (PUN47_12460) zapD 2678197..2678937 (+) 741 WP_024374405.1 cell division protein ZapD -
  PUN47_RS12465 (PUN47_12465) yacG 2678992..2679189 (+) 198 WP_020332195.1 DNA gyrase inhibitor YacG -
  PUN47_RS12470 (PUN47_12470) parC 2679240..2681513 (-) 2274 WP_274888547.1 DNA topoisomerase IV subunit A -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44698.97 Da        Isoelectric Point: 10.5318

>NTDB_id=793856 PUN47_RS12445 WP_104966495.1 2675406..2676632(+) (pilC) [Vibrio fluvialis strain 10M-VF]
MKPTKGPELKNYRWKGINSTGKKVAGQTLAISEIEVRDKLKEQHIQVKKIKKGSVSVLTRLTNRVKTKDITILTRQLATM
LTTGVPIVQALKLVGDNHRKAEMKSILAQITKGVEAGTPISKAMRTASTHFDALYVDLIQTGEQSGNLAEVFERLATYRE
KSEQLRAKVIKALIYPSMVILVALGVSYLMLTMVIPEFESMFKGFGAELPWFTQQILKLSHGVQAYSAAAFIASLGLVFG
IKAARKKSFVVRLKTSQLSLRFPIIGGVLAKAAIAKFSRTLATSFSAGIPILASLKTTAKTAGNVHFETAIQDVHRHTAA
GMPMYIAMRNTQAFPEMVLQMVMIGEESGKLDDMLNKVATIYEFEVDNTVDNLGKILEPLIIVFLGIVVGGLVVAMYLPI
FNLMSVLG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=793856 PUN47_RS12445 WP_104966495.1 2675406..2676632(+) (pilC) [Vibrio fluvialis strain 10M-VF]
ATGAAACCGACCAAAGGCCCTGAGTTAAAAAACTATCGCTGGAAAGGCATCAACAGCACTGGCAAGAAAGTCGCAGGACA
GACACTGGCTATCAGTGAAATAGAAGTCCGCGACAAACTGAAAGAACAGCACATTCAGGTTAAGAAGATAAAAAAGGGTA
GCGTGTCGGTTCTGACTCGTCTCACCAATCGGGTCAAAACTAAAGACATCACCATCCTAACCCGCCAGCTTGCCACCATG
CTGACCACCGGAGTGCCAATTGTGCAAGCACTCAAATTGGTCGGCGATAATCATCGTAAAGCCGAGATGAAATCAATTCT
GGCGCAAATTACCAAAGGCGTCGAAGCCGGTACGCCGATTTCCAAAGCGATGCGTACCGCCAGCACTCACTTTGACGCGT
TGTACGTCGACTTAATTCAGACTGGCGAGCAGTCCGGCAATCTTGCCGAAGTCTTTGAGCGCCTTGCCACCTATCGGGAA
AAAAGCGAACAACTGCGCGCGAAAGTAATCAAAGCGCTGATCTATCCCAGCATGGTCATTCTGGTGGCACTCGGCGTCTC
CTATTTGATGCTGACGATGGTCATTCCAGAGTTCGAAAGTATGTTCAAAGGTTTTGGGGCTGAACTGCCTTGGTTTACTC
AGCAGATCCTCAAGTTGTCGCACGGGGTACAAGCCTACAGTGCAGCGGCATTCATCGCGTCATTGGGCTTGGTGTTTGGC
ATCAAAGCGGCGCGGAAAAAATCCTTCGTCGTCCGTCTTAAAACCAGCCAGCTCAGCCTGCGTTTTCCCATCATTGGCGG
TGTGCTTGCCAAAGCCGCAATTGCCAAATTCAGCCGCACTCTGGCGACCAGCTTCAGTGCGGGAATTCCGATACTCGCCA
GCCTCAAAACCACTGCTAAAACGGCAGGTAACGTGCATTTTGAAACCGCCATTCAGGATGTTCACCGCCATACGGCGGCA
GGGATGCCGATGTATATTGCAATGCGCAACACACAAGCCTTTCCGGAAATGGTGTTGCAAATGGTGATGATCGGCGAGGA
GTCCGGCAAGCTCGACGACATGCTCAATAAAGTCGCCACCATCTATGAGTTTGAAGTCGACAACACCGTCGACAATCTGG
GCAAAATTCTTGAACCGCTGATTATCGTATTTTTGGGCATTGTGGTCGGTGGATTGGTGGTGGCGATGTACCTGCCAATC
TTTAACTTAATGAGTGTATTAGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

87.255

100

0.873
  pilC Vibrio campbellii strain DS40M4

72.593

99.265

0.721
  pilC Acinetobacter baumannii D1279779

41.422

100

0.414
  pilC Legionella pneumophila strain ERS1305867

41.604

97.794

0.407
  pilC Acinetobacter baylyi ADP1

41.058

97.304

0.4
  pilC Pseudomonas stutzeri DSM 10701

40.152

97.059

0.39
  pilG Neisseria gonorrhoeae MS11

39.599

97.794

0.387
  pilG Neisseria meningitidis 44/76-A

39.098

97.794

0.382