Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   PUN47_RS12450 Genome accession   NZ_CP118599
Coordinates   2676684..2677553 (+) Length   289 a.a.
NCBI ID   WP_044366750.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 10M-VF     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2671684..2682553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN47_RS12430 (PUN47_12430) nadC 2672126..2673013 (+) 888 WP_020430449.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PUN47_RS12435 (PUN47_12435) pilA 2673244..2673675 (+) 432 WP_154184015.1 pilin Machinery gene
  PUN47_RS12440 (PUN47_12440) pilB 2673682..2675370 (+) 1689 WP_274888545.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PUN47_RS12445 (PUN47_12445) pilC 2675406..2676632 (+) 1227 WP_104966495.1 type II secretion system F family protein Machinery gene
  PUN47_RS12450 (PUN47_12450) pilD 2676684..2677553 (+) 870 WP_044366750.1 prepilin peptidase Machinery gene
  PUN47_RS12455 (PUN47_12455) coaE 2677556..2678164 (+) 609 WP_104966494.1 dephospho-CoA kinase -
  PUN47_RS12460 (PUN47_12460) zapD 2678197..2678937 (+) 741 WP_024374405.1 cell division protein ZapD -
  PUN47_RS12465 (PUN47_12465) yacG 2678992..2679189 (+) 198 WP_020332195.1 DNA gyrase inhibitor YacG -
  PUN47_RS12470 (PUN47_12470) parC 2679240..2681513 (-) 2274 WP_274888547.1 DNA topoisomerase IV subunit A -

Sequence

Protein


Download         Length: 289 a.a.        Molecular weight: 32288.40 Da        Isoelectric Point: 6.4655

>NTDB_id=793857 PUN47_RS12450 WP_044366750.1 2676684..2677553(+) (pilD) [Vibrio fluvialis strain 10M-VF]
MDIFNFYPWLFPTLATVLGLIIGSFLNVVIYRLPKIMEREWRQECAESFPEYHIEPPQGILTLSMPRSTCPHCDTPIRVI
DNIPVLSWLLLRGKCSNCQGPISARYPLIELLTASCSLLIALQFGFSWFAVAMLFFTYTLIAATFIDFDTMLLPDQLTLP
LLWAGIALALAGISPISLQDAVIGAIAGYLSLWSVYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPIIILMSSLVGL
VFGLIQLRLQKRGIDKAFPFGPYLAIAGWLSLLFGHDIAGWYFHSVLGF

Nucleotide


Download         Length: 870 bp        

>NTDB_id=793857 PUN47_RS12450 WP_044366750.1 2676684..2677553(+) (pilD) [Vibrio fluvialis strain 10M-VF]
ATGGACATTTTTAATTTTTACCCCTGGCTGTTTCCTACGCTGGCGACAGTGCTGGGGTTAATTATCGGCAGTTTCCTCAA
TGTGGTGATTTATCGCCTGCCGAAAATCATGGAGCGCGAGTGGCGTCAGGAGTGTGCGGAATCCTTTCCCGAGTATCATA
TTGAGCCGCCTCAGGGCATTTTAACGCTGAGCATGCCACGTTCCACCTGCCCGCATTGCGATACGCCGATCCGGGTTATC
GACAACATTCCGGTACTGAGTTGGCTATTGCTGCGTGGTAAATGCTCGAATTGTCAGGGCCCTATCAGCGCGCGTTATCC
GCTGATTGAACTGCTGACGGCTTCATGCAGCCTCTTGATTGCGCTGCAATTTGGTTTTAGCTGGTTTGCCGTTGCCATGC
TGTTCTTTACCTATACGCTGATTGCGGCAACCTTCATTGATTTCGATACGATGCTGCTGCCGGATCAGCTTACTCTGCCC
CTACTCTGGGCAGGTATTGCACTGGCGTTGGCGGGCATCAGCCCGATTTCGCTACAAGATGCGGTGATTGGTGCGATAGC
AGGTTATCTGTCGTTATGGTCAGTGTACTGGCTGTTTAAGCTACTGACAGGCAAAGAAGGCATGGGCTATGGCGACTTTA
AGCTGTTAGCGGCTCTCGGTGCCTGGCTGGGATGGCAGCAACTACCGATCATCATTCTGATGTCATCTCTGGTTGGACTG
GTGTTCGGCCTCATTCAACTGCGTCTGCAAAAGCGCGGCATTGATAAAGCATTTCCCTTCGGGCCTTATCTGGCGATTGC
AGGCTGGTTATCCCTGCTGTTTGGCCATGACATTGCGGGTTGGTATTTTCATTCGGTATTAGGATTCTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(135-244)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

78.819

99.654

0.785
  pilD Vibrio campbellii strain DS40M4

78.125

99.654

0.779
  pilD Acinetobacter baumannii D1279779

53.285

94.81

0.505
  pilD Acinetobacter nosocomialis M2

52.92

94.81

0.502
  pilD Neisseria gonorrhoeae MS11

50.929

93.08

0.474