Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PUN47_RS12440 Genome accession   NZ_CP118599
Coordinates   2673682..2675370 (+) Length   562 a.a.
NCBI ID   WP_274888545.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 10M-VF     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2668682..2680370
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN47_RS12410 (PUN47_12410) dsbC 2669066..2669830 (-) 765 WP_020430455.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  PUN47_RS12415 (PUN47_12415) xerD 2669847..2670755 (-) 909 WP_020331983.1 site-specific tyrosine recombinase XerD -
  PUN47_RS12420 (PUN47_12420) fldB 2670896..2671417 (+) 522 WP_020430452.1 flavodoxin FldB -
  PUN47_RS12425 (PUN47_12425) ampD 2671422..2671973 (-) 552 WP_032081227.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PUN47_RS12430 (PUN47_12430) nadC 2672126..2673013 (+) 888 WP_020430449.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PUN47_RS12435 (PUN47_12435) pilA 2673244..2673675 (+) 432 WP_154184015.1 pilin Machinery gene
  PUN47_RS12440 (PUN47_12440) pilB 2673682..2675370 (+) 1689 WP_274888545.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PUN47_RS12445 (PUN47_12445) pilC 2675406..2676632 (+) 1227 WP_104966495.1 type II secretion system F family protein Machinery gene
  PUN47_RS12450 (PUN47_12450) pilD 2676684..2677553 (+) 870 WP_044366750.1 prepilin peptidase Machinery gene
  PUN47_RS12455 (PUN47_12455) coaE 2677556..2678164 (+) 609 WP_104966494.1 dephospho-CoA kinase -
  PUN47_RS12460 (PUN47_12460) zapD 2678197..2678937 (+) 741 WP_024374405.1 cell division protein ZapD -
  PUN47_RS12465 (PUN47_12465) yacG 2678992..2679189 (+) 198 WP_020332195.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 61982.92 Da        Isoelectric Point: 6.1969

>NTDB_id=793855 PUN47_RS12440 WP_274888545.1 2673682..2675370(+) (pilB) [Vibrio fluvialis strain 10M-VF]
MLTNLLAILRQADLLSPTQEQVVMDHIHASGTNVPEALIDLAIFNHHDLVEHISQIFGLPNTDLTRFDFAPLCQQLGLRE
LITRYQALPLNSNGQVLTLAVSDPTHLQVEDEFRFATGLQVELVLADHKALQAAIRRLYGRAIASHGTQGKEISQDELAS
LVQVSDDEKTSIEDLSQDDSPVSRFINQILLDAVRKGASDIHFEPYESFYRIRLRCDGILVEIQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLNADTAIDMRVSTLPTLWGEKIVLRLLDSSASSLDIDKLGYNDAQKQLYLAALTKPQGMI
LMTGPTGSGKTVSLYTGLRILNTDEVNISTAEDPVEINLPGINQVQVQPKIGFGFAQALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGIEPFNLASSLSLIIAQRLARRLCPHCKVATSPSPTLQQQFHLSSQQ
PIYAANPSGCNECTNGYSGRVGIYEVMAFNSALAEAIMQRASIHQIESIAQQHGMQTLLQSGIEKLRAGITSFSELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=793855 PUN47_RS12440 WP_274888545.1 2673682..2675370(+) (pilB) [Vibrio fluvialis strain 10M-VF]
ATGCTCACCAACCTACTCGCCATCCTGCGCCAGGCCGATTTGCTTAGCCCTACGCAGGAACAGGTCGTGATGGATCATAT
TCACGCTTCAGGAACCAATGTTCCGGAAGCGTTGATCGATTTGGCGATCTTTAATCACCACGATCTGGTCGAACATATCA
GCCAGATCTTTGGTTTGCCGAATACTGATCTAACCCGTTTTGACTTCGCGCCGCTGTGCCAGCAGTTGGGGCTGCGCGAG
CTCATCACCCGTTATCAGGCTCTGCCCCTCAACAGCAACGGTCAGGTACTGACTCTTGCGGTATCCGATCCTACTCACCT
ACAAGTTGAAGACGAGTTTCGCTTTGCGACCGGGTTACAAGTAGAACTGGTATTGGCTGATCATAAGGCACTGCAAGCTG
CTATTCGCCGCTTGTATGGCCGAGCCATCGCGTCACATGGCACGCAGGGCAAGGAGATCAGCCAGGACGAACTGGCCAGT
CTGGTGCAGGTTTCCGATGACGAAAAAACATCAATCGAAGATCTGAGTCAGGATGACTCCCCGGTCAGTCGTTTCATCAA
CCAGATCCTGCTGGATGCGGTGCGTAAAGGCGCTTCTGATATCCACTTTGAGCCCTATGAATCCTTTTATCGCATTCGCC
TGCGCTGCGACGGTATTTTGGTGGAAATCCAGCAACCAGCCAGCCATTTAAGCCGACGACTGGCTGCGCGGATTAAGATC
TTGTCGAAACTCGATATCGCCGAACGACGTTTACCCCAGGACGGCCGCATCAAATTACGCCTCAATGCGGACACTGCCAT
TGATATGCGGGTCTCCACTCTGCCAACCTTGTGGGGGGAGAAAATCGTCCTGCGTCTGCTCGACAGCAGTGCGTCCAGCC
TCGATATCGACAAGTTGGGTTATAACGATGCGCAAAAGCAGCTTTATCTGGCGGCCCTCACCAAGCCGCAAGGCATGATT
CTGATGACTGGCCCGACGGGCAGCGGTAAGACAGTCTCACTCTATACCGGCCTTCGTATTCTCAATACCGACGAAGTGAA
TATTTCTACCGCCGAAGATCCGGTGGAAATTAACCTGCCCGGCATTAATCAGGTTCAGGTGCAGCCCAAAATTGGTTTTG
GATTTGCGCAGGCTCTGCGCTCCTTTCTGCGTCAGGATCCCGATGTGGTGATGGTGGGCGAAATCCGCGATTTGGAAACG
GCAGAGATTGCCGTCAAAGCCGCCCAAACCGGCCACTTGGTGCTATCCACACTGCACACCAACTCCGCCGCCGAAACCGT
GATTCGGCTGGCGAACATGGGCATTGAACCGTTCAACCTCGCTTCATCGCTAAGCCTCATCATCGCTCAACGACTTGCCC
GGCGCCTTTGCCCTCATTGCAAAGTCGCCACATCGCCCTCTCCGACACTGCAGCAACAATTTCACTTGAGTTCTCAGCAG
CCTATCTATGCCGCCAACCCATCAGGCTGCAACGAGTGCACCAACGGTTACAGCGGGCGGGTGGGGATTTATGAAGTGAT
GGCATTTAACTCCGCGCTGGCTGAAGCGATTATGCAACGAGCCAGCATCCATCAGATCGAATCCATTGCCCAGCAACATG
GCATGCAAACGCTGTTGCAATCGGGGATCGAAAAACTTCGCGCCGGGATCACCAGTTTCAGTGAGTTGCAGCGTGTTCTC
TATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

82.206

100

0.822
  pilB Vibrio campbellii strain DS40M4

72.42

100

0.724
  pilB Vibrio parahaemolyticus RIMD 2210633

71.708

100

0.717
  pilB Legionella pneumophila strain ERS1305867

52.611

91.993

0.484
  pilB Acinetobacter baumannii D1279779

50.482

92.349

0.466
  pilB Acinetobacter baylyi ADP1

49.146

93.772

0.461
  pilF Neisseria gonorrhoeae MS11

44.681

100

0.448
  pilF Thermus thermophilus HB27

37.544

100

0.375
  pilB/pilB1 Synechocystis sp. PCC 6803

37.276

99.288

0.37