Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   PUW74_RS01140 Genome accession   NZ_CP118080
Coordinates   197798..198769 (+) Length   323 a.a.
NCBI ID   WP_000250805.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain VSI14     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 192798..203769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW74_RS01130 (PUW74_01130) rpoC 193496..197146 (+) 3651 WP_000228729.1 DNA-directed RNA polymerase subunit beta' -
  PUW74_RS01135 (PUW74_01135) - 197260..197625 (+) 366 WP_000285373.1 DUF1033 family protein -
  PUW74_RS01140 (PUW74_01140) comYA 197798..198769 (+) 972 WP_000250805.1 competence type IV pilus ATPase ComGA Machinery gene
  PUW74_RS01145 (PUW74_01145) comYB 198663..199706 (+) 1044 WP_223295889.1 competence type IV pilus assembly protein ComGB Machinery gene
  PUW74_RS01150 (PUW74_01150) comGC/cglC 199703..200032 (+) 330 WP_000793380.1 competence type IV pilus major pilin ComGC Machinery gene
  PUW74_RS01155 (PUW74_01155) comYD 200007..200420 (+) 414 WP_000244261.1 competence type IV pilus minor pilin ComGD Machinery gene
  PUW74_RS01160 (PUW74_01160) comGE 200392..200691 (+) 300 WP_000234804.1 competence type IV pilus minor pilin ComGE -
  PUW74_RS01165 (PUW74_01165) comGF 200645..201106 (+) 462 WP_001874812.1 competence type IV pilus minor pilin ComGF -
  PUW74_RS01170 (PUW74_01170) comGG 201084..201455 (+) 372 WP_000601104.1 competence type IV pilus minor pilin ComGG -
  PUW74_RS01175 (PUW74_01175) comYH 201570..202544 (+) 975 WP_001008574.1 N-6 DNA methylase Machinery gene
  PUW74_RS01180 (PUW74_01180) - 202576..203769 (+) 1194 WP_000047534.1 acetate kinase -

Sequence

Protein


Download         Length: 323 a.a.        Molecular weight: 36801.32 Da        Isoelectric Point: 6.8469

>NTDB_id=789932 PUW74_RS01140 WP_000250805.1 197798..198769(+) (comYA) [Streptococcus agalactiae strain VSI14]
MVQSLAKQVIHQAVEVNAQDIYIIPKGDCYELYMRIDDERRFIDVFEFNRMASLISHFKFVAGMNVGEKRRSQLGSCDYE
LSEGRLVSLRLSSVGDYRGQESLVIRILYSGHQDLKYWFDNIKQMKEVLGIRGLYLFSGPVGSGKTTLMYQLASEVFKNK
QIITIEDPVEIKNDKMLQLQLNEDIGMTYDALIKLSLRHRPDILIIGEIRDQATARAVIRASLTGVMVFSTIHAKSIPGV
YDRLIELGVNYQELENSLKLIAYQRLIGGGSLIDFETGNFKKHSSDKWNRQVDILAEEGHISKKQAQVEKIIPQETTESS
PTF

Nucleotide


Download         Length: 972 bp        

>NTDB_id=789932 PUW74_RS01140 WP_000250805.1 197798..198769(+) (comYA) [Streptococcus agalactiae strain VSI14]
ATGGTTCAATCATTAGCAAAGCAAGTCATTCATCAGGCAGTAGAAGTAAATGCTCAAGATATTTATATCATTCCCAAAGG
TGATTGTTATGAACTCTATATGCGTATTGATGATGAAAGGCGGTTTATTGATGTTTTTGAGTTTAATAGGATGGCTAGTC
TTATTAGTCACTTTAAATTTGTGGCAGGCATGAACGTTGGAGAAAAAAGACGAAGTCAATTAGGTTCTTGTGACTATGAA
CTGTCAGAGGGAAGACTGGTTTCATTACGACTATCGAGTGTGGGAGATTATCGTGGTCAAGAATCTTTAGTTATTCGTAT
TTTGTATTCAGGTCATCAGGACTTAAAATATTGGTTTGATAATATAAAGCAAATGAAGGAAGTACTGGGTATAAGAGGGC
TATATCTTTTTTCCGGCCCTGTGGGGAGTGGTAAAACAACTCTCATGTATCAATTAGCTTCAGAAGTATTTAAAAATAAG
CAAATTATCACGATTGAAGATCCGGTAGAAATCAAGAATGACAAGATGTTACAACTCCAATTGAATGAGGATATTGGAAT
GACTTATGATGCTTTAATCAAACTGTCTTTACGGCATCGTCCAGATATTTTAATTATCGGAGAGATTAGAGATCAAGCGA
CGGCCCGTGCTGTTATTCGTGCAAGTTTAACGGGAGTGATGGTTTTTTCTACTATTCATGCTAAAAGTATTCCCGGAGTC
TATGATAGGCTTATAGAATTAGGGGTTAACTATCAAGAGTTAGAAAATAGTCTAAAATTAATAGCATATCAACGTTTAAT
TGGAGGAGGAAGCCTAATTGACTTTGAGACAGGTAATTTTAAAAAACACTCATCAGACAAGTGGAATAGACAAGTGGATA
TCTTGGCTGAAGAAGGACATATCAGTAAGAAACAGGCACAAGTCGAAAAAATTATCCCTCAAGAAACAACGGAAAGTAGT
CCAACTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

68.81

96.285

0.663
  comYA Streptococcus mutans UA140

68.81

96.285

0.663
  comYA Streptococcus gordonii str. Challis substr. CH1

63.636

98.762

0.628
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

62.701

96.285

0.604
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

61.736

96.285

0.594
  comGA/cglA/cilD Streptococcus pneumoniae D39

61.736

96.285

0.594
  comGA/cglA/cilD Streptococcus pneumoniae R6

61.736

96.285

0.594
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

61.736

96.285

0.594
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.935

95.975

0.594
  comGA Lactococcus lactis subsp. cremoris KW2

50.621

99.69

0.505